Planapo 63x/1.4, DIC, 320µm length, 5 stacks stitched in Photoshop, Marine sample, Alboran Sea.
Image is linked from imgur as it is 2200 x 580 pixels. Those of you with a large screen will be able to see a little more detail.
Licmophora sp.
Licmophora sp.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Licmophora sp.
'Pinch Zoom' on the iPad works fine
... astonishing level of detail visible.
MichaelG.
... astonishing level of detail visible.
MichaelG.
Too many 'projects'
Re: Licmophora sp.
Thanks MichaelG.
The virtues of stitching!... astonishing level of detail visible.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Licmophora sp.
The amount of detail is amazing. I absolutely enjoy your diatom survey of the Alboran Sea. You are really good at stacking and stitching! How do you ensure the object stays static while you collect the multiple sets of images? Do you seal the edges of the coverslip?
Out of curiosity how do you collect all the awesome diatoms you post? Do you scrape off underwater stones or use a plankton net?
Out of curiosity how do you collect all the awesome diatoms you post? Do you scrape off underwater stones or use a plankton net?
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Licmophora sp.
Thanks Wes
In this case I also pressed down on the cover slip a little too firmly, as can be seen by the results.
I use a 6.3x to scan the slide and up to 40x dry to take images until the cover slip is low enough for the WD of the 63x/1.4, assuming there is something worth getting everything oily for!
*Alga washed ashore also contains plenty of diatoms but the alga gets rather smelly very quickly!
I have scraped off alga from stones on a couple of occasions, it also works well.
To place the sample on the slide I grab/tear off a small clump of alga with tweezers and tap it lightly several times on the slide.
This provides plenty to see (though to the naked eye the drop/drops may appear empty) without crowding the subjects.
I don't seal the edges of the cover slip as I need the water to evaporate. I add drops with tweezers when needed.How do you ensure the object stays static while you collect the multiple sets of images? Do you seal the edges of the coverslip?
In this case I also pressed down on the cover slip a little too firmly, as can be seen by the results.
I use a 6.3x to scan the slide and up to 40x dry to take images until the cover slip is low enough for the WD of the 63x/1.4, assuming there is something worth getting everything oily for!
So far I am finding plenty of variation picking alga up from the surf (just before it is washed ashore*) or removing alga from the ropes that dangle in the water at the local marina.Out of curiosity how do you collect all the awesome diatoms you post? Do you scrape off underwater stones or use a plankton net?
*Alga washed ashore also contains plenty of diatoms but the alga gets rather smelly very quickly!
I have scraped off alga from stones on a couple of occasions, it also works well.
To place the sample on the slide I grab/tear off a small clump of alga with tweezers and tap it lightly several times on the slide.
This provides plenty to see (though to the naked eye the drop/drops may appear empty) without crowding the subjects.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)