Amphora sp. (probably Amphora arcus)

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75RR
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Amphora sp. (probably Amphora arcus)

#1 Post by 75RR » Fri Sep 20, 2019 10:33 am

Planapo 63x/1.4, DIC, 90 µm, stacked in Photoshop, Marine sample, Alboran Sea.

top = images 600-642 , bottom = images 635-672 and all = images 600-672

probably Amphora arcus (to be confirmed)
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Last edited by 75RR on Fri Sep 20, 2019 2:27 pm, edited 1 time in total.
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mintakax
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Re: Amphora sp. (probably Amphora securicula)

#2 Post by mintakax » Fri Sep 20, 2019 1:03 pm

Wow ! These images are amazing. I'm, curious how much time do images like this require ? Assuming one is proficient with the appropriate PS techniques ?
Dan

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75RR
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Re: Amphora sp. (probably Amphora securicula)

#3 Post by 75RR » Fri Sep 20, 2019 2:45 pm

.
Note: I have amended the first post to reflect my new probable ID from Amphora securicula to Amphora arcus

mintakax wrote:
Fri Sep 20, 2019 1:03 pm
I'm, curious how much time do images like this require ? Assuming one is proficient with the appropriate PS techniques ?
Thanks mintakax,

I did not do the whole process in one go but if I had it would have taken a couple of hours.

The photographic session took about an hour from when I spotted the diatom and took the first images to when I went back to it later to see if it had slowed down enough to try again.

I could have done it in less if I had leached the water out rather than let it evaporate, but I was not sure what else was on the slide.

Post processing in Camara raw + photoshop took around an hour or so for the 3 images.
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Re: Amphora sp. (probably Amphora arcus)

#4 Post by Wes » Sun Sep 22, 2019 1:18 pm

Great images 75RR!

Do you know how big are the steps in your z-stack? I find that 1 µm (or smaller) vertical steps are ok for diatoms, otherwise I get artifacts in the striae stitching.

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75RR
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Re: Amphora sp. (probably Amphora arcus)

#5 Post by 75RR » Sun Sep 22, 2019 2:01 pm

Thanks Wes

A little less than one is probably about right.

Had a look just now at the amount of movement I get within the 2µm marks (microscope side on and reproducing the rotation feel) and reckon I get about 3 images per 2µm


On a slightly related issue, I have been disappointed with how little my new batteries seemed to last, until that is I read somewhere that they are supposed to give around 300 shots per charge!

So I stopped moaning, as it's not the battery - it's the fine focus ;)
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Re: Amphora sp. (probably Amphora arcus)

#6 Post by Wes » Sun Sep 22, 2019 4:00 pm

How many miliampere hours is your new battery and how many is the old one? I hate it when there is some cool microbe or something exciting going on, then the battery dies and I have no replacement.

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Re: Amphora sp. (probably Amphora arcus)

#7 Post by 75RR » Sun Sep 22, 2019 4:16 pm

How many miliampere hours is your new battery and how many is the old one?
Note to self: Have to stop buying cheapest available!


I hate it when there is some cool microbe or something exciting going on, then the battery dies and I have no replacement.
Murphy's Law. He visits me as well
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Re: Amphora sp. (probably Amphora arcus)

#8 Post by Hobbyst46 » Sun Sep 22, 2019 6:45 pm

Wes wrote:...
75RR wrote:...
Folks, are there no AC adapters for Olympus cameras ? for my Canon I bought a (~20-30$ or even less, cannot remember) a 220V AC adapter. A Chinese thing. And I never touched the battery anymore, now for nearly 2 years.
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Re: Amphora sp. (probably Amphora arcus)

#9 Post by 75RR » Mon Sep 23, 2019 5:43 am

Folks, are there no AC adapters for Olympus cameras ? for my Canon I bought a (~20-30$ or even less, cannot remember) a 220V AC adapter. A Chinese thing.
And I never touched the battery anymore, now for nearly 2 years.
Had a look at one point but did not find anything for the Olympus Ep-2
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Re: Amphora sp. (probably Amphora arcus)

#10 Post by Wes » Tue Sep 24, 2019 6:18 pm

Hobbyst46 wrote:
Sun Sep 22, 2019 6:45 pm
Wes wrote:...
75RR wrote:...
Folks, are there no AC adapters for Olympus cameras ? for my Canon I bought a (~20-30$ or even less, cannot remember) a 220V AC adapter. A Chinese thing. And I never touched the battery anymore, now for nearly 2 years.
I meant to make a thread asking exactly about this but now I know what I'm looking for. Thanks!

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