Stauroneis phoenicenteron

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Wes
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Stauroneis phoenicenteron

#1 Post by Wes » Sun Sep 22, 2019 9:58 pm

A stack of 6 images with total stack depth of 6 µm. 40/0,95 objective DIC.

The diatom is from an 8 form test plate by Klaus Kemp (whom I've not been able to contact recently, I hope he's ok).

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BR
Wes

Timemaster1212
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Re: Stauroneis phoenicenteron

#2 Post by Timemaster1212 » Sun Sep 22, 2019 11:28 pm

Very beautiful photo Wes! Love the depth. And I do hope Klaus Kemp is doing well.

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75RR
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Location: Estepona

Re: Stauroneis phoenicenteron

#3 Post by 75RR » Mon Sep 23, 2019 4:49 am

Very nicely done!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Wes
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Re: Stauroneis phoenicenteron

#4 Post by Wes » Mon Sep 23, 2019 5:18 am

Thank you Timemaster1212 and 75RR. Rotating the stage such that the longitudinal axis of the diatom is at 90 degrees to the DIC axis of shearing improved resolution of the pores quite a bit.

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75RR
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Re: Stauroneis phoenicenteron

#5 Post by 75RR » Mon Sep 23, 2019 5:47 am

Rotating the stage such that the longitudinal axis of the diatom is at 90 degrees to the DIC axis of shearing improved resolution of the pores quite a bit.
Indeed! Very useful for Pol, DIC and Oblique

Here are some instructions for centering the stage:
Attachments
Stage Centering.jpg
Stage Centering.jpg (106.01 KiB) Viewed 778 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

mintakax
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Re: Stauroneis phoenicenteron

#6 Post by mintakax » Tue Sep 24, 2019 12:39 am

I love the phenomenal detail Wes and the rotation discussion. Thanks !


Wes
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Re: Stauroneis phoenicenteron

#8 Post by Wes » Tue Sep 24, 2019 6:15 pm

75RR wrote:
Mon Sep 23, 2019 5:47 am
Rotating the stage such that the longitudinal axis of the diatom is at 90 degrees to the DIC axis of shearing improved resolution of the pores quite a bit.
Indeed! Very useful for Pol, DIC and Oblique

Here are some instructions for centering the stage:
On my microscope I need to have two special keys that have square holes with a 2 mm side. I found a substitute that randomly fitted ok and used it to center the stage but it wasn't super straightforward, I had to do a bunch of corrections until it find a workable center.
mintakax wrote:
Tue Sep 24, 2019 12:39 am
I love the phenomenal detail Wes and the rotation discussion. Thanks !
ImperatorRex wrote:
Tue Sep 24, 2019 4:42 am
Phantastic photo Wes.
Thank you Dan and Jochen, I am currently looking into making permanent diatom slides and make more photos like this.

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