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Thanks 75RR . I dropped a boiled corn kernel into the small Petri dish with the sample
Thanks ! It was a flat slide. I examine slide with a stereo microscope and if no large creatures are present I put on a slide cover.Hobbyst46 wrote: ↑Wed Sep 25, 2019 7:01 amA very attractive video!
- was the specimen mounted on a well slide or a regular flat slide ?
- there are elongated, cylindical creatures with "mouths" that remind of rotifers. are those flagellates ?
- (same ignorance in critters) I fail to notice the amoebas, would you direct to a location in the image if I pause the video at a given moment ?
Thanks for the video and thanks in advance for answers.
Thank you ! I can say that the sample originated from the smelly ooze at the shore of a local pond. The original sample went into a covered plastic milk jug that I keep alongside my "pond aquarium", then I put some detritus from that jug into a petri dish with some of the water and dropped a kernel of boiled corn into that. That culture is 10 days old. The water in it is thick and "milky". As for the stability, I have no idea. I really don't know what I'm doingTimemaster1212 wrote: ↑Wed Sep 25, 2019 1:12 pmLovely video! You keep mentioning you dropped the kernel into a sample, however where was the sample from? I am assuming a local pond no? If so how stable is such a culture?
mintakax wrote: ↑Wed Sep 25, 2019 2:37 pmThank you ! I can say that the sample originated from the smelly ooze at the shore of a local pond. The original sample went into a covered plastic milk jug that I keep alongside my "pond aquarium", then I put some detritus from that jug into a petri dish with some of the water and dropped a kernel of boiled corn into that. That culture is 10 days old. The water in it is thick and "milky". As for the stability, I have no idea. I really don't know what I'm doing
Yes, lovely (and lively!) video.Sauerkraut wrote: ↑Wed Sep 25, 2019 4:10 pmNice video and DIC.
Could that long, thin protist be Spirostomum?
Thanks Bruce-- I was pursuing Fresh-water Biology (Ward and Whipple) and thought that Urochaemia might be a possibility ?Bruce Taylor wrote: ↑Wed Sep 25, 2019 11:46 pmYes, lovely (and lively!) video.Sauerkraut wrote: ↑Wed Sep 25, 2019 4:10 pmNice video and DIC.
Could that long, thin protist be Spirostomum?
No, the long ciliates are not Spirostomum, but a species of vermiform hypotrich. Mobile cirri and a spirotrich-type adoral zone of membranelles are visible at times, especially around the 1:13 mark. I am doubtful that we can identify them below subclass level. There are quite a few long, skinny hypotrichs, from a variety of genera...e.g. Hemisincirra, Vermioxytricha, Circinella, Engelmanniella mobilis.
Thank you Wes ! Interesting that you seen flagellate swarms as well. The size of the individuals in my video were around 15 um average. Are you seeing a lot of small amoebas and cysts and flagellates that look like they are all related ? The thought that I am breeding a culture of Naegleria Fowleri (the brain eating amoeba) is creeping me out. But much of what I am seeing supports this. I guess this is a common amoeba ?Wes wrote: ↑Wed Sep 25, 2019 6:22 pmLovely cinematographic work, I really enjoy these videos! I find it very peculiar that you see swarms of tiny flagellates because thats exactly what I saw after doing a boiled corn culture simultaneously with you back a week or two ago. Probably a just a coincidence, towards the later days of the culture I could already see very large ciliates with the naked eye but assumed they're paramecia and didn't investigate further. Overall it made me wonder whether bacteria come first, then small eukaryotes because of their small genomes and large surface to volume ratio giving them a replicative speed advantage and eventually the bigger microbes get to the apex of the food chain.
Here is what the tiny flagellates I found look like. They're about 10 µm in size (sort of averaged), have 2 flagella that I could see, happy to eat bacteria (can see some wrapped up in food vacuoles) and possess a orange eyespot near the flagella base.
Thanks Heather !Sauerkraut wrote: ↑Wed Sep 25, 2019 4:10 pmNice video and DIC.
Could that long, thin protist be Spirostomum?
I am really glad that there are people like you around BruceBruce Taylor wrote: ↑Thu Sep 26, 2019 1:22 amNope.These are certainly hypotrichs, and Urochaenia is an enchelyid (in a whole different subclass).
. I am failing so miserably at identifying these microorganisms. Hypotrich is close enough for me and its great to have something more detailed than protozoa for a change !I never saw any amoeba in this particular sample. I did however see tiny worms covered in cilia that had stolen intact stinging cells from what I presume to be Hydra (apparently much more common occurrence that I imagined).mintakax wrote: ↑Thu Sep 26, 2019 12:17 amThank you Wes ! Interesting that you seen flagellate swarms as well. The size of the individuals in my video were around 15 um average. Are you seeing a lot of small amoebas and cysts and flagellates that look like they are all related ? The thought that I am breeding a culture of Naegleria Fowleri (the brain eating amoeba) is creeping me out. But much of what I am seeing supports this. I guess this is a common amoeba ?Wes wrote: ↑Wed Sep 25, 2019 6:22 pmLovely cinematographic work, I really enjoy these videos! I find it very peculiar that you see swarms of tiny flagellates because thats exactly what I saw after doing a boiled corn culture simultaneously with you back a week or two ago. Probably a just a coincidence, towards the later days of the culture I could already see very large ciliates with the naked eye but assumed they're paramecia and didn't investigate further. Overall it made me wonder whether bacteria come first, then small eukaryotes because of their small genomes and large surface to volume ratio giving them a replicative speed advantage and eventually the bigger microbes get to the apex of the food chain.
Here is what the tiny flagellates I found look like. They're about 10 µm in size (sort of averaged), have 2 flagella that I could see, happy to eat bacteria (can see some wrapped up in food vacuoles) and possess a orange eyespot near the flagella base.