Colurella sp

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Colurella sp

#1 Post by 75RR » Sun Sep 29, 2019 4:36 am

Planapo 63x/1.4, DIC, stacked in Photoshop, seasonal river waterhole, Southern Spain
Attachments
Colurella sp.jpg
Colurella sp.jpg (183.8 KiB) Viewed 601 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

tgss
Posts: 86
Joined: Wed Jan 25, 2017 3:48 am
Location: Ontario, Canada

Re: Colurella sp

#2 Post by tgss » Sun Sep 29, 2019 2:51 pm

I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a bit :?
Congrats on yet another excellent image.
Tom W.

User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Re: Colurella sp

#3 Post by 75RR » Sun Sep 29, 2019 4:43 pm

Thanks tgss, it helps that it is an unusually rich sample.

Here is another rotifer from it. This time a Brachionus

It was surrounded by moving euglenas, not sure if they were attracted by the light, the prospect of a meal or both.

+ one of the suspects. This is a good example of why I should finish the flash project.
Attachments
Brachionus.jpg
Brachionus.jpg (187.63 KiB) Viewed 551 times
430ab.jpg
430ab.jpg (101.92 KiB) Viewed 551 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
ImperatorRex
Posts: 223
Joined: Fri Mar 30, 2018 4:12 pm
Location: Germany, Karlsruhe
Contact:

Re: Colurella sp

#4 Post by ImperatorRex » Sun Sep 29, 2019 6:21 pm

Hi 75RR,
very nice photos.
I have filtered to remove some noise ... hope you like it.

Image

Image

User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Re: Colurella sp

#5 Post by 75RR » Sun Sep 29, 2019 6:41 pm

Does look better, thanks. Gaussian blur?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
ImperatorRex
Posts: 223
Joined: Fri Mar 30, 2018 4:12 pm
Location: Germany, Karlsruhe
Contact:

Re: Colurella sp

#6 Post by ImperatorRex » Sun Sep 29, 2019 7:04 pm

It is the software DENOISE PROJECTS. It is reducing the noise but also many other kind of automized corrections.

User avatar
KurtM
Posts: 1514
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Colurella sp

#7 Post by KurtM » Mon Sep 30, 2019 12:48 am

75RR is the wizard of the Zeiss 63x/1.4 Planapo for sure. If I ever get back in my lab I may be hitting him up for advice - mine gives me fits!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
http://sawdustfactory.nfshost.com/microscopes/
https://www.flickr.com/photos/67904872@ ... 912223623/

Sauerkraut
Posts: 196
Joined: Tue Apr 16, 2019 2:07 am
Location: Oregon, USA

Re: Colurella sp

#8 Post by Sauerkraut » Mon Sep 30, 2019 4:01 pm

That's a really nice side view of Colurella. Excellent detail.

I bet you could get nice and differing images from that one waterhole location indefinitely.

User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Re: Colurella sp

#9 Post by 75RR » Mon Sep 30, 2019 5:53 pm

Thanks KurtM and Sauerkraut

Must admit I have become rather fond of the Zeiss 63x/1.4 Planapo

Will definitely revisit the waterhole!
It is the software DENOISE PROJECTS. It is reducing the noise but also many other kind of automized corrections.
Thanks for the name, did not realize there was such specialist software.

I shall be putting a little more effort into reducing noise both during the shoot and after.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
ImperatorRex
Posts: 223
Joined: Fri Mar 30, 2018 4:12 pm
Location: Germany, Karlsruhe
Contact:

Re: Colurella sp

#10 Post by ImperatorRex » Mon Sep 30, 2019 7:07 pm

Hi 75RR,
do not get me wrong - I like your photos very much and I do not really care about some noise in the photos.
But if you want to remove I thought it may be a good tip. Actually I do not use Photoshop anymore since I tried the DENOISE software. It allows a kind of automatisation, so several steps like constrast, noise reduction or sharpening in a sequence. So its quite convenient. It offers a 4 week trial period to have a first try.

User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Re: Colurella sp

#11 Post by 75RR » Mon Sep 30, 2019 7:23 pm

No problem, I appreciate the new look!

I won't be getting the software however but rather looking to camera settings and lighting to reduce noise and additional Photoshop buttons to smooth the background a some more.

A little trial and error should help things along. Thanks again
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Wes
Posts: 307
Joined: Sat Mar 09, 2019 12:58 pm

Re: Colurella sp

#12 Post by Wes » Mon Sep 30, 2019 7:42 pm

Hi 75', I find that using the 100W halogen lamp is my best bet at reducing noise. It provides about enough light to shoot well lit photos at ISO 100 and 1/80 shutter speed (around this speed vibrations don't cause much issues). If I remember correctly you were missing a piece of the 100W lamp house and you didn't want to burn your 60W (I also try to be economical with the voltage when using the 60W bulb).

mintakax
Posts: 167
Joined: Mon Jul 29, 2019 12:06 am
Location: Boulder CO, USA

Re: Colurella sp

#13 Post by mintakax » Fri Oct 04, 2019 1:47 pm

Just saw this post. Amazing detail, wow !

mintakax
Posts: 167
Joined: Mon Jul 29, 2019 12:06 am
Location: Boulder CO, USA

Re: Colurella sp

#14 Post by mintakax » Fri Oct 04, 2019 1:47 pm

Is that 63x oil or dry ?

User avatar
75RR
Posts: 6241
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona

Re: Colurella sp

#15 Post by 75RR » Fri Oct 04, 2019 2:48 pm

Thanks Wes and mintakax
If I remember correctly you were missing a piece of the 100W lamp house and you didn't want to burn your 60W
Indeed. I think by now I should admit to myself that I am unlikely to source a focusing lens at a fair price for the 100W halogen. Seems a shame, as the rest of it is fine.
Is that 63x oil or dry ?
It is a rather fat and heavy Planapochromat 63x/1.4 Oel

Weighed it on the kitchen scales = 130gr
Attachments
Planapo.jpg
Planapo.jpg (98.07 KiB) Viewed 424 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Post Reply