Wait for it !

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mintakax
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Wait for it !

#1 Post by mintakax » Tue Oct 29, 2019 4:07 am

This has to be one of the coolest, weirdest and most unexpected things I've seen since I bought my microscope. I was watching what I thought was a nuclearia (branched, not stiff pseudopods with no extrusions) when the pseudopods started to pulse... then the organism just disappeared (first clip in video) . I found it again, way on the other side of the slide and then ......
Second half of video is a repeat at reduced speed. Not sure of ID beyond testate filose.

Apologies for the back and forth, there was quite a bit of action to follow for a 40x objective.

https://vimeo.com/369482992

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75RR
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Re: Wait for it !

#2 Post by 75RR » Tue Oct 29, 2019 4:40 am

That was surprising! Great catch and great video. Any idea of its size?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

mintakax
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Re: Wait for it !

#3 Post by mintakax » Tue Oct 29, 2019 4:50 am

75RR wrote:
Tue Oct 29, 2019 4:40 am
That was surprising! Great catch and great video. Any idea of its size?
Thanks 75RR-- There is a few seconds of a size metric at the beginning, but I would say the top view of the spherical shell is about 20um, the total size including pseudopods is ~60um. When walking, the length of the test is ~28um and max width is ~20um.

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75RR
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Re: Wait for it !

#4 Post by 75RR » Tue Oct 29, 2019 4:57 am

mintakax wrote:
Tue Oct 29, 2019 4:50 am
75RR wrote:
Tue Oct 29, 2019 4:40 am
That was surprising! Great catch and great video. Any idea of its size?
Thanks 75RR-- There is a few seconds of a size metric at the beginning, but I would say the top view of the spherical shell is about 20um, the total size including pseudopods is ~60um. When walking, the length of the test is ~28um and max width is ~20um.
Sorry! Just watched again and so there is. Bad habit of mine to hover the cursor over the stop start button.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Wes
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Re: Wait for it !

#5 Post by Wes » Tue Oct 29, 2019 8:23 am

Now that is an unusual sighting! Thanks for sharing.

Bruce Taylor
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Re: Wait for it !

#6 Post by Bruce Taylor » Tue Oct 29, 2019 11:55 am

Fun! This is the ciliate Mesodinium. What you were seeing as pseudopods are the long cirri around its "waist," and a set of specialized stiff cirri called "jumping bristles." As you can see, they work pretty well. :D I think the cirri are a bit frayed, which is why they look like they are "branching".

mintakax
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Re: Wait for it !

#7 Post by mintakax » Tue Oct 29, 2019 2:05 pm

Bruce Taylor wrote:
Tue Oct 29, 2019 11:55 am
Fun! This is the ciliate Mesodinium. What you were seeing as pseudopods are the long cirri around its "waist," and a set of specialized stiff cirri called "jumping bristles." As you can see, they work pretty well. :D I think the cirri are a bit frayed, which is why they look like they are "branching".
Thanks Wes and Bruce !

Well, I never would have guessed this was a ciliate. Amazing little creature even if it does remind me of a tick :)

Bruce Taylor
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Re: Wait for it !

#8 Post by Bruce Taylor » Tue Oct 29, 2019 2:24 pm

Some ciliates can jump so quickly that it looks like they're teleporting! :D

Chris Dee
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Re: Wait for it !

#9 Post by Chris Dee » Tue Oct 29, 2019 3:38 pm

And always when your finger is on the shutter release. Great vid, thanks for sharing.

MicroBob
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Re: Wait for it !

#10 Post by MicroBob » Tue Oct 29, 2019 6:23 pm

Great Find!
I have never seen this before. It looks completely different from the side.
Also very quick are these vorticella: https://vimeo.com/263421396

Bob

PeteM
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Re: Wait for it !

#11 Post by PeteM » Tue Oct 29, 2019 8:40 pm

Makes me think the "transformers" kids play with aren't entirely science fiction . . .

mintakax
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Re: Wait for it !

#12 Post by mintakax » Tue Oct 29, 2019 9:24 pm

MicroBob wrote:
Tue Oct 29, 2019 6:23 pm
Great Find!
I have never seen this before. It looks completely different from the side.
Also very quick are these vorticella: https://vimeo.com/263421396

Bob
Thanks Bob-- Wow 1200fps ! Thats serious high speed. Sometimes I try and record at 60 fps, but then cant do 4K.

mintakax
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Re: Wait for it !

#13 Post by mintakax » Tue Oct 29, 2019 9:27 pm

Bruce Taylor wrote:
Tue Oct 29, 2019 2:24 pm
Some ciliates can jump so quickly that it looks like they're teleporting! :D
I cant believe how far away this creature jumped, it was seriously 2/3 the length of the slide. Makes me wonder how much "relative" vertical space there is under that slip cover. There was a fair bit of sand in that sample drop so maybe there was ample space.

mintakax
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Re: Wait for it !

#14 Post by mintakax » Tue Oct 29, 2019 9:31 pm

PeteM wrote:
Tue Oct 29, 2019 8:40 pm
Makes me think the "transformers" kids play with aren't entirely science fiction . . .
I love the transformer analogy !

mintakax
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Re: Wait for it !

#15 Post by mintakax » Tue Oct 29, 2019 9:32 pm

Chris Dee wrote:
Tue Oct 29, 2019 3:38 pm
And always when your finger is on the shutter release. Great vid, thanks for sharing.
Thanks Chris !

MicroBob
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Re: Wait for it !

#16 Post by MicroBob » Tue Oct 29, 2019 9:36 pm

At 1200 fps this is really low resolution, far from 4k or even HD. But it makes the movement visible. Some smartphones do 120 fps at high resolution.

charlie g
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Re: Wait for it !

#17 Post by charlie g » Sun Nov 03, 2019 7:11 pm

Thanks, mintakax, for a great encounter..is the illumination method a type of phase-contrast? Please speak a bit on this illumination method you used. thanls, Charlie guevara

mintakax
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Re: Wait for it !

#18 Post by mintakax » Sun Nov 03, 2019 9:39 pm

charlie g wrote:
Sun Nov 03, 2019 7:11 pm
Thanks, mintakax, for a great encounter..is the illumination method a type of phase-contrast? Please speak a bit on this illumination method you used. thanls, Charlie guevara
Thank you Charlie-- This is straight DIC. I adjust the prism to get the best contrast between subject and background.

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