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Planapo 63x/1.4, COL and DIC, 100µm length stacked in Photoshop
This is from a Klaus Kemp 8 Form slide
Not the fairest of comparisons as I used the position III phase annulus in the condenser to achieve COL and it is a little small for best results with the 63x
Stauroneis phoenicenteron (COL-DIC)
Stauroneis phoenicenteron (COL-DIC)
- Attachments
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- Stauroneis phoenicenteron(COL-DIC).jpg (151.64 KiB) Viewed 2138 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Stauroneis phoenicenteron (COL-DIC)
Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.
Tom W.
Re: Stauroneis phoenicenteron (COL-DIC)
Thanks tgss
Even though the COL would be better with a larger annulus I think it still shows it to be a good step up on brightfield.
Even though the COL would be better with a larger annulus I think it still shows it to be a good step up on brightfield.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Stauroneis phoenicenteron (COL-DIC)
Both images are very nice !
On the one hand, the DIC appears to be 3D, on the other hand, I remember that it is not necessarily true.
The COL on the other hand is quite similar to phase contrast. I guess that it is nearer the "true" image (where true=SEM, but only my guess).
On the one hand, the DIC appears to be 3D, on the other hand, I remember that it is not necessarily true.
The COL on the other hand is quite similar to phase contrast. I guess that it is nearer the "true" image (where true=SEM, but only my guess).
Re: Stauroneis phoenicenteron (COL-DIC)
Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.
Tom W.