Licmophora flabellata

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Licmophora flabellata

#1 Post by 75RR » Wed Jan 22, 2020 9:05 pm

.
Planapo 63x/1.4, DIC, 125µm length (each diatom), stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Love the symmetry
Attachments
Licmophora flabellata.jpg
Licmophora flabellata.jpg (210.86 KiB) Viewed 2047 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
mrsonchus
Posts: 3682
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Licmophora flabellata

#2 Post by mrsonchus » Wed Jan 22, 2020 9:24 pm

Simply Stunning - almost looks like a Japanese fan. A
beautiful image, superbly executed - your standards always leave me astonished 75'.
John B

WhyMe
Posts: 147
Joined: Fri Dec 27, 2019 10:04 pm
Location: Eastern USA

Re: Licmophora flabellata

#3 Post by WhyMe » Wed Jan 22, 2020 9:59 pm

Wow very nice! :)

Wes
Posts: 479
Joined: Sat Mar 09, 2019 12:58 pm

Re: Licmophora flabellata

#4 Post by Wes » Wed Jan 22, 2020 11:27 pm

Amazing shot!

How did you fix the background noise in the image?

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Licmophora flabellata

#5 Post by 75RR » Thu Jan 23, 2020 7:00 am

Many thanks mrsonchus, WhyMe and Wes
How did you fix the background noise in the image?
Pretty much as is, as due to the number of stacks, this photo is only about 10% of the stitched image.

So effectively, a lot of very mini pixels.
Attachments
Screen Shot.jpg
Screen Shot.jpg (160.12 KiB) Viewed 1999 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Hobbyst46
Posts: 2576
Joined: Mon Aug 21, 2017 9:02 pm

Re: Licmophora flabellata

#6 Post by Hobbyst46 » Thu Jan 23, 2020 1:08 pm

Impressive as usual !
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

mintakax
Posts: 268
Joined: Mon Jul 29, 2019 12:06 am
Location: Boulder CO, USA

Re: Licmophora flabellata

#7 Post by mintakax » Fri Jan 24, 2020 4:36 am

Simply amazing Glen ! Do you use a micro manipulator ?

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Licmophora flabellata

#8 Post by 75RR » Fri Jan 24, 2020 5:32 am

Many thanks Hobbyst46 and mintakax
Do you use a micro manipulator ?
No I don't, though I suppose that would be the next step.

I do however barely tap the alga on the cover slip (which transfers just a drop or two of water), so that when I find something worthwhile it is usually on its own and there is very little detritus to stop the cover slip laying nice and flat.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

MicroBob
Posts: 1976
Joined: Sun Dec 25, 2016 9:11 am
Location: Northern Germany

Re: Licmophora flabellata

#9 Post by MicroBob » Fri Jan 24, 2020 9:18 am

Hi Glen,
great image!
It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?

Bob

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Licmophora flabellata

#10 Post by 75RR » Fri Jan 24, 2020 12:08 pm

Thanks MicroBob
It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?
Will be happy to make the comparison test, however the advantage of stitching is that it allows one to photograph a subject with both higher magnification and higher NA

Even when reduced to the same size as that taken by a lower power there will still be more detail available.

Will try with either the 63x/1.4 and the 40x/1.0 or the 40x/0.65 and the 25x/0.60
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
KurtM
Posts: 1554
Joined: Tue Jun 09, 2015 12:08 am
Location: League City, Texas
Contact:

Re: Licmophora flabellata

#11 Post by KurtM » Sat Jan 25, 2020 12:54 am

Fabulous image, Glen! :shock:
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)aol(dot)com
http://sawdustfactory.nfshost.com/microscopes/
https://www.flickr.com/photos/67904872@ ... 912223623/

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Licmophora flabellata

#12 Post by 75RR » Sat Jan 25, 2020 3:09 pm

Thanks Kurt!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Post Reply