MicrobeHunter.com Microscopy Forum

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Planapo 63x/1.4, DIC, 125µm length (each diatom), stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Love the symmetry

Simply Stunning - almost looks like a Japanese fan. A
beautiful image, superbly executed - your standards always leave me astonished 75'.

Wow very nice!

Amazing shot!

How did you fix the background noise in the image?

Many thanks mrsonchus, WhyMe and Wes

How did you fix the background noise in the image?

Pretty much as is, as due to the number of stacks, this photo is only about 10% of the stitched image.

So effectively, a lot of very mini pixels.

Impressive as usual !

Simply amazing Glen ! Do you use a micro manipulator ?

Many thanks Hobbyst46 and mintakax

Do you use a micro manipulator ?

No I don't, though I suppose that would be the next step.

I do however barely tap the alga on the cover slip (which transfers just a drop or two of water), so that when I find something worthwhile it is usually on its own and there is very little detritus to stop the cover slip laying nice and flat.

Hi Glen,
great image!
It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?

Bob

Thanks MicroBob

It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?

Will be happy to make the comparison test, however the advantage of stitching is that it allows one to photograph a subject with both higher magnification and higher NA

Even when reduced to the same size as that taken by a lower power there will still be more detail available.

Will try with either the 63x/1.4 and the 40x/1.0 or the 40x/0.65 and the 25x/0.60

Fabulous image, Glen!

Thanks Kurt!