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Licmophora flabellata

Posted: Wed Jan 22, 2020 9:05 pm
by 75RR
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Planapo 63x/1.4, DIC, 125┬Ám length (each diatom), stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Love the symmetry

Re: Licmophora flabellata

Posted: Wed Jan 22, 2020 9:24 pm
by mrsonchus
Simply Stunning - almost looks like a Japanese fan. A
beautiful image, superbly executed - your standards always leave me astonished 75'.

Re: Licmophora flabellata

Posted: Wed Jan 22, 2020 9:59 pm
by WhyMe
Wow very nice! :)

Re: Licmophora flabellata

Posted: Wed Jan 22, 2020 11:27 pm
by Wes
Amazing shot!

How did you fix the background noise in the image?

Re: Licmophora flabellata

Posted: Thu Jan 23, 2020 7:00 am
by 75RR
Many thanks mrsonchus, WhyMe and Wes
How did you fix the background noise in the image?
Pretty much as is, as due to the number of stacks, this photo is only about 10% of the stitched image.

So effectively, a lot of very mini pixels.

Re: Licmophora flabellata

Posted: Thu Jan 23, 2020 1:08 pm
by Hobbyst46
Impressive as usual !

Re: Licmophora flabellata

Posted: Fri Jan 24, 2020 4:36 am
by mintakax
Simply amazing Glen ! Do you use a micro manipulator ?

Re: Licmophora flabellata

Posted: Fri Jan 24, 2020 5:32 am
by 75RR
Many thanks Hobbyst46 and mintakax
Do you use a micro manipulator ?
No I don't, though I suppose that would be the next step.

I do however barely tap the alga on the cover slip (which transfers just a drop or two of water), so that when I find something worthwhile it is usually on its own and there is very little detritus to stop the cover slip laying nice and flat.

Re: Licmophora flabellata

Posted: Fri Jan 24, 2020 9:18 am
by MicroBob
Hi Glen,
great image!
It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?

Bob

Re: Licmophora flabellata

Posted: Fri Jan 24, 2020 12:08 pm
by 75RR
Thanks MicroBob
It seems that stitching increases image quality even when it is reduced to 1024 pixel web image size afterwards. In theory it should be fine to use whatever objective covers the whole object in one frame. There must be a physical reason for this. When you find your next object that you want to photograph with stitching, can you take a single image with a lower power objective too for comparison?
Will be happy to make the comparison test, however the advantage of stitching is that it allows one to photograph a subject with both higher magnification and higher NA

Even when reduced to the same size as that taken by a lower power there will still be more detail available.

Will try with either the 63x/1.4 and the 40x/1.0 or the 40x/0.65 and the 25x/0.60

Re: Licmophora flabellata

Posted: Sat Jan 25, 2020 12:54 am
by KurtM
Fabulous image, Glen! :shock:

Re: Licmophora flabellata

Posted: Sat Jan 25, 2020 3:09 pm
by 75RR
Thanks Kurt!