Nitzschia sigmoidea

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Nitzschia sigmoidea

#1 Post by 75RR » Sun Feb 09, 2020 9:13 am

Planapo 63x/1.4, DIC, 250µm length, stacked and stitched in Photoshop, Marine sample, Alboran Sea.
Attachments
Nitzschia sigmoidea(250µm)-.jpg
Nitzschia sigmoidea(250µm)-.jpg (50.9 KiB) Viewed 3336 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

MichaelG.
Posts: 1904
Joined: Mon Apr 10, 2017 8:24 am
Location: NorthWest England

Re: Nitzschia sigmoidea

#2 Post by MichaelG. » Sun Feb 09, 2020 10:21 am

Fabulous image !

At this resolution I can’t see any evidence of the stitching :shock:
... is it really that good ?

MichaelG.
Too many 'projects'

User avatar
mrsonchus
Posts: 3682
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Nitzschia sigmoidea

#3 Post by mrsonchus » Sun Feb 09, 2020 10:51 am

MichaelG. wrote:
Sun Feb 09, 2020 10:21 am
Fabulous image !

At this resolution I can’t see any evidence of the stitching :shock:
... is it really that good ?

MichaelG.
Ditto Michael!
It really is - when combined with 75's consummate skill with this work - fantastic inspiration and goal for others.
John B

Hobbyst46
Posts: 2574
Joined: Mon Aug 21, 2017 9:02 pm

Re: Nitzschia sigmoidea

#4 Post by Hobbyst46 » Sun Feb 09, 2020 1:00 pm

Great image (as usual) !!

The diatoms seem to feed well on the western side of the Mediterranean, and grow to such size. :|
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

WhyMe
Posts: 147
Joined: Fri Dec 27, 2019 10:04 pm
Location: Eastern USA

Re: Nitzschia sigmoidea

#5 Post by WhyMe » Sun Feb 09, 2020 2:36 pm

Very nice!

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Nitzschia sigmoidea

#6 Post by 75RR » Sun Feb 09, 2020 3:59 pm

Many thanks MichaelG, mrsonchus, Hobbyst46 and WhyMe
At this resolution I can’t see any evidence of the stitching
Photoshop stitching (photomerge) is quite seamless and easy to use. Choose images - press button.

If there are problems it is usually either a lack of sufficient image overlap or non matching overlaps.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Wes
Posts: 479
Joined: Sat Mar 09, 2019 12:58 pm

Re: Nitzschia sigmoidea

#7 Post by Wes » Sun Feb 09, 2020 6:18 pm

Beautiful work 75'

How do you do the stitching? I tried to load the files to be stacked via "File>Script>Load Files Into Stack" and then "Edit>Auto-Align Layers".

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Nitzschia sigmoidea

#8 Post by 75RR » Sun Feb 09, 2020 6:45 pm

Thanks Wes

I normally use Adobe Bridge, I find it easier.

If you are going to go direct then there are 3 steps:

"File>Script>Load Files Into Stack"
"Edit>Auto-Align Layers"
"Edit>Auto-Blend Layers> Panorama"

Note: You need to highlight all files that are to be processed.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
mrsonchus
Posts: 3682
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Nitzschia sigmoidea

#9 Post by mrsonchus » Sun Feb 09, 2020 7:52 pm

Hi 75',
I've just started using Bridge/Photoshop (and Lightroom-Classic for fast batch edits) and have found the stacking to be pretty good in PS also. Is there a way to know what type of stacking PS is performing, or even maybe to alter parameters do you know? Perhaps the absence of parameters points to 'Pyramid' stacking?
John B

User avatar
75RR
Posts: 6793
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Nitzschia sigmoidea

#10 Post by 75RR » Sun Feb 09, 2020 8:58 pm

Hi mrsonchus, can't say I have found anyway to fine tune Photoshop stacking/stitching (seems to me to be mostly automated) nor do I know how it works. Shame on me!

I started using Photoshop for this as I already had it. I find it useful, but suspect it is a little basic as it is just another addition to what is a massive photo editing software program.

I believe dedicated programs are necessarily better, but I always seem to find another use for the money needed for one.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
mrsonchus
Posts: 3682
Joined: Tue Feb 03, 2015 9:42 pm
Location: Cumbria, UK

Re: Nitzschia sigmoidea

#11 Post by mrsonchus » Sun Feb 09, 2020 9:09 pm

I think you're right 75' - I definitely see different effects with the various (Weighted-Average, Depth-Map, Pyramid) options in Helicon Focus - especially I suspect with the very often 'soft' images of obscure detail within a mounted-section rather than optimally at the coverslip's underside.
John B

Post Reply