Hypotrich Ciliate is having lunch

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daruosha
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Hypotrich Ciliate is having lunch

#1 Post by daruosha » Fri Mar 06, 2020 1:11 am

Today I went to the same aquarium shop again and after showing some pictures and videos from previous samples, they enthusiastically offered me 8 more samples and asked me to see what creatures are in the water samples.

One of their samples was something they called "Algae Bloom". I centrifuged 15ml of the sample for 10 minutes at 3500 RPM and after removing 14ml of the supernatant, carefully took a drop from precipitated residue. Here is what one of the shots I captured with my DSLR.

Image
40x NA 0.65 - Condenser aperture fully open - Oblique disc diameter 17mm - Canon EOS 50D

My DSLR pictures are crap, I'm very disappointed. I don't know why, but they are soft and suffer from serious lack of sharpness, despite making it para-focal with eyepieces and everything I've done, they are blurry and soft. (I use mirror lockup and 2sec delay timer as well).

I hope you like the pictures, more is to come :)

PS: To be honest, my pictures are very bad compared to what other members regularly post on the forum. I feel embarrassed. Do you have any suggestion or recommendation?
Last edited by daruosha on Tue Mar 10, 2020 11:35 am, edited 3 times in total.
Daruosh.

MicroBob
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Re: Coleps is having lunch

#2 Post by MicroBob » Fri Mar 06, 2020 3:50 am

Hi Daroush,
to me the image looks fairly typical, perhaps the colour of the algae is a bit subdued. The algae are probably Selenastrum, sometimes used as food for fish larvae.
Your camera is fine for this application. Do you use the right silent mode and release from live view? You also might check whether focussing over the camera screen is more precise.

Bob

einman
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Re: Coleps is having lunch

#3 Post by einman » Fri Mar 06, 2020 4:15 am

I don't believe that is a Coleps. It is however a very good photo.

Here is an old video I made of Coleps eating.


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daruosha
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Re: Coleps is having lunch

#4 Post by daruosha » Fri Mar 06, 2020 4:23 am

einman wrote:
Fri Mar 06, 2020 4:15 am
I don't believe that is a Coleps. It is however a very good photo.

Here is an old video I made of Coleps eating.

Nice video. Background music suits it too.

What do think it is?
Daruosh.

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KD Arvidsson
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Re: Coleps (or may be a Protozoa) is having lunch

#5 Post by KD Arvidsson » Fri Mar 06, 2020 10:20 pm

Great photo Daruosha😊. //KD
Microscope Nikon Labophot 2
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw

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daruosha
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Re: Coleps is having lunch

#6 Post by daruosha » Fri Mar 06, 2020 11:36 pm

MicroBob wrote:
Fri Mar 06, 2020 3:50 am
Hi Daroush,
to me the image looks fairly typical, perhaps the colour of the algae is a bit subdued. The algae are probably Selenastrum, sometimes used as food for fish larvae.
Your camera is fine for this application. Do you use the right silent mode and release from live view? You also might check whether focussing over the camera screen is more precise.

Bob
Yes, They are indeed Selenastrum. Confirmed by aquarium shop man.

I found why my DSLR images were horrible. There was a protective plastic film inside the reduction lens. I mean literally INSIDE the lens.
Image
I took it apart, removed the plastic film and after that pictures look MUCH MUCH better (of course).

I do photography for more than 10 years and I'm quite familiar with my cameras (I have a 5D III too and it had the same bad image quality on microscope too (D'oh), my reduction lens only covers APC-C form factor though) and I knew something was wrong outside the camera.
Daruosh.

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daruosha
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Re: Coleps (or may be a Protozoa) is having lunch

#7 Post by daruosha » Fri Mar 06, 2020 11:37 pm

KD Arvidsson wrote:
Fri Mar 06, 2020 10:20 pm
Great photo Daruosha😊. //KD
Thank you KD.
Daruosh.

MicroBob
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Re: Coleps is having lunch

#8 Post by MicroBob » Sat Mar 07, 2020 7:17 am

daruosha wrote:
Fri Mar 06, 2020 11:36 pm
There was a protective plastic film inside the reduction lens.
Funny error! A kind of intelligence test. Think about how many people will already have thrown away these reduction lenses as they didn't find the hidden problem. :(

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75RR
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Re: Coleps (or may be a Protozoa) is having lunch

#9 Post by 75RR » Sat Mar 07, 2020 11:10 am

Think about how many people will already have thrown away these reduction lenses as they didn't find the hidden problem.
Perhaps there is something to be said in favor of reading instructions after all. ;)
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

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daruosha
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Re: Coleps (or may be a Protozoa) is having lunch

#10 Post by daruosha » Sat Mar 07, 2020 10:01 pm

75RR wrote:
Sat Mar 07, 2020 11:10 am
Think about how many people will already have thrown away these reduction lenses as they didn't find the hidden problem.
Perhaps there is something to be said in favor of reading instructions after all. ;)
This is the adapter: no branding, no name, Chinesium look, no manual :D
Image

Image

I have to find a better adapter. Can I project the photo eyepiece image directly on camera sensor?
Daruosh.

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75RR
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Re: Coleps (or may be a Protozoa) is having lunch

#11 Post by 75RR » Sat Mar 07, 2020 10:11 pm

Can I project the photo eyepiece image directly on camera sensor?
Yes you can. You will need to raise the eyepiece a few mm for it to work.

These are the spacers I used when I did that. They are made from electrical wall pipes - they are a perfect fit.

Sizes are: 2.5mm, 5mm, 1mm and 2mm

That gives me plenty of combinations to play around with.
Attachments
Spacers-.jpg
Spacers-.jpg (34.91 KiB) Viewed 8539 times
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Bruce Taylor
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Re: Coleps is having lunch

#12 Post by Bruce Taylor » Tue Mar 10, 2020 1:17 am

daruosha wrote:
Fri Mar 06, 2020 4:23 am
What do think it is?
It is a hypotrich ciliate. We only see a few of its somatic cirri, and the cell is somewhat compressed, so identification below subclass isn't possible.

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daruosha
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Re: Coleps is having lunch

#13 Post by daruosha » Tue Mar 10, 2020 11:36 am

Bruce Taylor wrote:
Tue Mar 10, 2020 1:17 am
daruosha wrote:
Fri Mar 06, 2020 4:23 am
What do think it is?
It is a hypotrich ciliate. We only see a few of its somatic cirri, and the cell is somewhat compressed, so identification below subclass isn't possible.
Huge thanks Bruce.
Daruosh.

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