Hypotrich Ciliate is having lunch
Hypotrich Ciliate is having lunch
Today I went to the same aquarium shop again and after showing some pictures and videos from previous samples, they enthusiastically offered me 8 more samples and asked me to see what creatures are in the water samples.
One of their samples was something they called "Algae Bloom". I centrifuged 15ml of the sample for 10 minutes at 3500 RPM and after removing 14ml of the supernatant, carefully took a drop from precipitated residue. Here is what one of the shots I captured with my DSLR.
40x NA 0.65 - Condenser aperture fully open - Oblique disc diameter 17mm - Canon EOS 50D
My DSLR pictures are crap, I'm very disappointed. I don't know why, but they are soft and suffer from serious lack of sharpness, despite making it para-focal with eyepieces and everything I've done, they are blurry and soft. (I use mirror lockup and 2sec delay timer as well).
I hope you like the pictures, more is to come
PS: To be honest, my pictures are very bad compared to what other members regularly post on the forum. I feel embarrassed. Do you have any suggestion or recommendation?
One of their samples was something they called "Algae Bloom". I centrifuged 15ml of the sample for 10 minutes at 3500 RPM and after removing 14ml of the supernatant, carefully took a drop from precipitated residue. Here is what one of the shots I captured with my DSLR.
40x NA 0.65 - Condenser aperture fully open - Oblique disc diameter 17mm - Canon EOS 50D
My DSLR pictures are crap, I'm very disappointed. I don't know why, but they are soft and suffer from serious lack of sharpness, despite making it para-focal with eyepieces and everything I've done, they are blurry and soft. (I use mirror lockup and 2sec delay timer as well).
I hope you like the pictures, more is to come
PS: To be honest, my pictures are very bad compared to what other members regularly post on the forum. I feel embarrassed. Do you have any suggestion or recommendation?
Last edited by daruosha on Tue Mar 10, 2020 11:35 am, edited 3 times in total.
Daruosh.
Re: Coleps is having lunch
Hi Daroush,
to me the image looks fairly typical, perhaps the colour of the algae is a bit subdued. The algae are probably Selenastrum, sometimes used as food for fish larvae.
Your camera is fine for this application. Do you use the right silent mode and release from live view? You also might check whether focussing over the camera screen is more precise.
Bob
to me the image looks fairly typical, perhaps the colour of the algae is a bit subdued. The algae are probably Selenastrum, sometimes used as food for fish larvae.
Your camera is fine for this application. Do you use the right silent mode and release from live view? You also might check whether focussing over the camera screen is more precise.
Bob
Re: Coleps is having lunch
I don't believe that is a Coleps. It is however a very good photo.
Here is an old video I made of Coleps eating.
Here is an old video I made of Coleps eating.
- KD Arvidsson
- Posts: 368
- Joined: Wed Feb 12, 2020 6:47 pm
- Location: Sweden
Re: Coleps (or may be a Protozoa) is having lunch
Great photo Daruosha. //KD
Microscope Nikon Labophot 2
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw
Re: Coleps is having lunch
Yes, They are indeed Selenastrum. Confirmed by aquarium shop man.MicroBob wrote: ↑Fri Mar 06, 2020 3:50 amHi Daroush,
to me the image looks fairly typical, perhaps the colour of the algae is a bit subdued. The algae are probably Selenastrum, sometimes used as food for fish larvae.
Your camera is fine for this application. Do you use the right silent mode and release from live view? You also might check whether focussing over the camera screen is more precise.
Bob
I found why my DSLR images were horrible. There was a protective plastic film inside the reduction lens. I mean literally INSIDE the lens.
I took it apart, removed the plastic film and after that pictures look MUCH MUCH better (of course).
I do photography for more than 10 years and I'm quite familiar with my cameras (I have a 5D III too and it had the same bad image quality on microscope too (D'oh), my reduction lens only covers APC-C form factor though) and I knew something was wrong outside the camera.
Daruosh.
Re: Coleps (or may be a Protozoa) is having lunch
Perhaps there is something to be said in favor of reading instructions after all. ;)Think about how many people will already have thrown away these reduction lenses as they didn't find the hidden problem.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Coleps (or may be a Protozoa) is having lunch
This is the adapter: no branding, no name, Chinesium look, no manual
I have to find a better adapter. Can I project the photo eyepiece image directly on camera sensor?
Daruosh.
Re: Coleps (or may be a Protozoa) is having lunch
Yes you can. You will need to raise the eyepiece a few mm for it to work.Can I project the photo eyepiece image directly on camera sensor?
These are the spacers I used when I did that. They are made from electrical wall pipes - they are a perfect fit.
Sizes are: 2.5mm, 5mm, 1mm and 2mm
That gives me plenty of combinations to play around with.
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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Re: Coleps is having lunch
It is a hypotrich ciliate. We only see a few of its somatic cirri, and the cell is somewhat compressed, so identification below subclass isn't possible.
Re: Coleps is having lunch
Huge thanks Bruce.Bruce Taylor wrote: ↑Tue Mar 10, 2020 1:17 amIt is a hypotrich ciliate. We only see a few of its somatic cirri, and the cell is somewhat compressed, so identification below subclass isn't possible.
Daruosh.