Two ciliates from my plancton aquarium in phase contrast
Two ciliates from my plancton aquarium in phase contrast
Hi together,
I just bought a nice Neofluar 40 Ph2 phase contrast objective and gave it a try with some plancton mix from my plancton aquaria.
Here two ciliates that could have been captured better with a 100:1 objective but I was too lazy and the dwindling oil resources and all...
Bob
I just bought a nice Neofluar 40 Ph2 phase contrast objective and gave it a try with some plancton mix from my plancton aquaria.
Here two ciliates that could have been captured better with a 100:1 objective but I was too lazy and the dwindling oil resources and all...
Bob
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- Wimperntier Planktonaquarium Neofluar 40 Ph2 DSC_9707 1024.JPG (187.55 KiB) Viewed 2685 times
Last edited by MicroBob on Wed Apr 01, 2020 7:24 am, edited 1 time in total.
Re: Twi ciliates from my plancton aquarium in phase contrast
Great, Bob.
The 40X/0.75 Ph2 Neofluoar is great when free of delamination.
The 40X/0.75 Ph2 Neofluoar is great when free of delamination.
Re: Twi ciliates from my plancton aquarium in phase contrast
Hi Doron,
while both my 16:1 Neofluar and 40:1 achromat phase objectives are delaminated this one is fine!
Bob
while both my 16:1 Neofluar and 40:1 achromat phase objectives are delaminated this one is fine!
Bob
Re: Twi ciliates from my plancton aquarium in phase contrast
Nice to have a Neofluar PH2 40x/0.75
I have yet to add one to my Phase nosepiece
I have been remiss with my Phase of late. Must (metaphorically ) dust them off!
I have yet to add one to my Phase nosepiece
I have been remiss with my Phase of late. Must (metaphorically ) dust them off!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Two ciliates from my plancton aquarium in phase contrast
Hi Glen,
so far my phase contrast objective set was very limited, just a good 100:1 Plan and a delaminated Neofluar 16:1 which I haven't tried to repair yet. My 40:1 Zeiss Winkel is heavyly delaminated and it shows. So I was quite happy to get this nice objective in good condition for a good price.
I haven't compared DIC and phase a lot due to the lack of promising phase objectives. But I'm sure that each will have it's application where it excells.
Bob
so far my phase contrast objective set was very limited, just a good 100:1 Plan and a delaminated Neofluar 16:1 which I haven't tried to repair yet. My 40:1 Zeiss Winkel is heavyly delaminated and it shows. So I was quite happy to get this nice objective in good condition for a good price.
I haven't compared DIC and phase a lot due to the lack of promising phase objectives. But I'm sure that each will have it's application where it excells.
Bob
Re: Two ciliates from my plancton aquarium in phase contrast
Hi Bob,
40/0,75 Neofluar Ph2 is the only phase contrast objective I have and I too like it a lot. Its great for things like bacteria and algae but also protists especially amoeba.
40/0,75 Neofluar Ph2 is the only phase contrast objective I have and I too like it a lot. Its great for things like bacteria and algae but also protists especially amoeba.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Two ciliates from my plancton aquarium in phase contrast
I have been slowly building up my Phase Objective nosepiece.
I have strict price guidelines so it is a very slow process.
On there now is an achromat 10x/0.22, a Neofluar 16x/0.40, a Neofluar 25x/0.60 and an achromat 40x/0.65
Next on my list is a Neofluar 40x/0.75 and one day perhaps a Plan-Neofluar 63x/0.90
There is so little rush that I forget to look for them sometimes! Mind you I blame the DIC for that!
No delamination on any of them - which is how it should be.
I have strict price guidelines so it is a very slow process.
On there now is an achromat 10x/0.22, a Neofluar 16x/0.40, a Neofluar 25x/0.60 and an achromat 40x/0.65
Next on my list is a Neofluar 40x/0.75 and one day perhaps a Plan-Neofluar 63x/0.90
There is so little rush that I forget to look for them sometimes! Mind you I blame the DIC for that!
No delamination on any of them - which is how it should be.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Two ciliates from my plancton aquarium in phase contrast
I'd really like to get a 40/0.9 multi-immersion Plan-Neofluar Ph3. These are some of the finest lenses ever made. The 63/0.9 is also on my list.
Unrelated I'd also love to get a Jamin-Lebedeff kit but I guess I'll have to wait a very long time for that
Unrelated I'd also love to get a Jamin-Lebedeff kit but I guess I'll have to wait a very long time for that
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
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Re: Two ciliates from my plancton aquarium in phase contrast
Those are impressive. I can never see the cilia.
Re: Two ciliates from my plancton aquarium in phase contrast
Hi Don,
the cilia are accentuated by the phase contrast. Also I edited the image which increased their visibility.
You might try olbique illumination to enhance the visibility.
Bob
the cilia are accentuated by the phase contrast. Also I edited the image which increased their visibility.
You might try olbique illumination to enhance the visibility.
Bob