Pine needle-fluorescence,

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Seb28 · #1 Post by **Seb28** » Tue Apr 07, 2020 9:26 am

Two cross sections slices of pine needle staining in calcofluor white and acridine orange.

: b1.jpg (156.52 KiB) Viewed 4469 times

: b2.jpg (244.47 KiB) Viewed 4469 times

: b6.jpg (103.18 KiB) Viewed 4469 times

: b5 copy.jpg (215.84 KiB) Viewed 4469 times

: b3.jpg (77.17 KiB) Viewed 4469 times

daruosha · #2 Post by **daruosha** » Tue Apr 07, 2020 9:52 am

Wow. It's really stunning. Well done.

MicroBob · #3 Post by **MicroBob** » Tue Apr 07, 2020 11:16 am

Hi Seb,
great images! Are these permanent mounted or fresh?

Bob

tgss · #4 Post by **tgss** » Tue Apr 07, 2020 11:18 am

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.

Seb28 · #5 Post by **Seb28** » Tue Apr 07, 2020 2:23 pm

Thank you.

great images! Are these permanent mounted or fresh?

They were fixed in FAA,PEG1500 embbeded and hand cut.

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.

Yes ,acridine staining make differentiation of tissues.

MicroBob · #6 Post by **MicroBob** » Tue Apr 07, 2020 4:24 pm

Many mountants have quite a bit of autofluorescence which makes it a bit difficult to get contrasty slides for fluorescence application. Is PEG1500 usable a a mountant or just for embedding for sectioning? I compared a couple of mountants for blue excitation fluorescence here:
viewtopic.php?f=10&t=8211&p=71982&hilit ... nts#p71982

Also it is a question of compatibility between mountant and intermedium.
On the first image of this thread you can see acrivlavine that has moved into the mountant:
viewtopic.php?f=6&t=8206&p=76166&hilit= ... nce#p76166

Bob

KD Arvidsson · #7 Post by **KD Arvidsson** » Tue Apr 07, 2020 7:47 pm

Very impressive and wonderfully beautiful! //KD

tgss · #8 Post by **tgss** » Tue Apr 07, 2020 7:53 pm

Seb28 wrote: ↑
Tue Apr 07, 2020 2:23 pm
Thank you.

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.
Yes ,acridine staining make differentiation of tissues.

Thanks for the info Seb, you've given me something to think about and some "research" to do!
Tom W

DonSchaeffer · #9 Post by **DonSchaeffer** » Wed Apr 08, 2020 12:23 am

I honor these shots! How did you do this?

Wes · #10 Post by **Wes** » Wed Apr 08, 2020 6:09 am

These are really good! Thanks for posting Seb

What microscope/objectives did you use for these shots?

75RR · #11 Post by **75RR** » Wed Apr 08, 2020 6:34 am

Always nice to the more exotic lighting techniques being used. You have added colour to my day!

RobBerdan · #12 Post by **RobBerdan** » Fri Apr 10, 2020 11:41 pm

Very nice images of the plant sections.
Cheers
RB

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