Pine needle-fluorescence,
Pine needle-fluorescence,
Two cross sections slices of pine needle staining in calcofluor white and acridine orange.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Pine needle-fluorescence,
Hi Seb,
great images! Are these permanent mounted or fresh?
Bob
great images! Are these permanent mounted or fresh?
Bob
Re: Pine needle-fluorescence,
Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.
Tom W.
Re: Pine needle-fluorescence,
Thank you.
They were fixed in FAA,PEG1500 embbeded and hand cut.great images! Are these permanent mounted or fresh?
Yes ,acridine staining make differentiation of tissues.Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Pine needle-fluorescence,
Many mountants have quite a bit of autofluorescence which makes it a bit difficult to get contrasty slides for fluorescence application. Is PEG1500 usable a a mountant or just for embedding for sectioning? I compared a couple of mountants for blue excitation fluorescence here:
viewtopic.php?f=10&t=8211&p=71982&hilit ... nts#p71982
Also it is a question of compatibility between mountant and intermedium.
On the first image of this thread you can see acrivlavine that has moved into the mountant:
viewtopic.php?f=6&t=8206&p=76166&hilit= ... nce#p76166
Bob
viewtopic.php?f=10&t=8211&p=71982&hilit ... nts#p71982
Also it is a question of compatibility between mountant and intermedium.
On the first image of this thread you can see acrivlavine that has moved into the mountant:
viewtopic.php?f=6&t=8206&p=76166&hilit= ... nce#p76166
Bob
- KD Arvidsson
- Posts: 368
- Joined: Wed Feb 12, 2020 6:47 pm
- Location: Sweden
Re: Pine needle-fluorescence,
Very impressive and wonderfully beautiful! //KD
Microscope Nikon Labophot 2
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw
Re: Pine needle-fluorescence,
Thanks for the info Seb, you've given me something to think about and some "research" to do!Seb28 wrote: ↑Tue Apr 07, 2020 2:23 pmThank you.
Yes ,acridine staining make differentiation of tissues.Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.
Tom W
-
- Posts: 3354
- Joined: Sun Mar 22, 2020 10:06 am
- Location: Winnipeg, Manitoba, Canada
Re: Pine needle-fluorescence,
I honor these shots! How did you do this?
Re: Pine needle-fluorescence,
These are really good! Thanks for posting Seb
What microscope/objectives did you use for these shots?
What microscope/objectives did you use for these shots?
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Pine needle-fluorescence,
Always nice to the more exotic lighting techniques being used. You have added colour to my day!
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Pine needle-fluorescence,
Very nice images of the plant sections.
Cheers
RB
Cheers
RB