Flagellated Bacteria

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sinabro
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Flagellated Bacteria

#1 Post by sinabro » Mon Apr 13, 2020 12:00 am

100x objective +dic


apochronaut
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Re: Flagellated Bacteria

#2 Post by apochronaut » Mon Apr 13, 2020 12:54 am

Do you have any idea what they were? What was the source of the water? In some of the water sources I collect from yearly, I see numbers of what would seem similar to me but I have difficulty imaging the flagella. The DIC and I presume an apo objective certainly brings out some wanting details . I have the apo but not DIC, as of yet.
Up until now I have simply blocked together the rod and thread bacteria as " gliding bacteria".

great video. It certainly points out that bacteria are not as bland and pointless to gaze at as some might suggest.

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micro
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Re: Flagellated Bacteria

#3 Post by micro » Mon Apr 13, 2020 4:11 am

I did not know such a thing existed

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75RR
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Re: Flagellated Bacteria

#4 Post by 75RR » Mon Apr 13, 2020 5:38 am

Great imaging!

https://microbeonline.com/bacterial-fla ... -bacteria/
It certainly points out that bacteria are not as bland and pointless to gaze at as some might suggest.
I think many times beginners equate 'bacteria' with protozoa, much the same way that many of us use 'insect' and 'bug' as a catch all term.

As such, bacteria is not what they think it is and is therefore hardly an ideal starting point for an amateur microscopist.
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Wes
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Re: Flagellated Bacteria

#5 Post by Wes » Mon Apr 13, 2020 7:36 pm

Very nice documentation. I observed the same kind of bacteria (visually at least) the other day and could make out the flagella using a dry 40/.95 objective. Interestingly I could just about see it with a 40/.75 phase contrast objective.
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daruosha
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Re: Flagellated Bacteria

#6 Post by daruosha » Mon Apr 13, 2020 8:10 pm

The footage is certainly top notch. Never seen anything like that.

Is there any chance that I can find some flagellated bacteria too? I really want to observe it myself.
Daruosh.

DonSchaeffer
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Re: Flagellated Bacteria

#7 Post by DonSchaeffer » Mon Apr 13, 2020 8:12 pm

Fantastic (and nightmarish) video.

apochronaut
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Re: Flagellated Bacteria

#8 Post by apochronaut » Mon Apr 13, 2020 10:50 pm

daruosha wrote:
Mon Apr 13, 2020 8:10 pm
The footage is certainly top notch. Never seen anything like that.

Is there any chance that I can find some flagellated bacteria too? I really want to observe it myself.
They seem to abound in oxygen depleted waters but are quite common in general. Many pathogenic bacteria sport flagella but in some cases they are not used for locomotion but for adherence.

Slow moving waters or those stalled due to dry weather should provide some. Dirty, scummy ponds. Anywhere close to any form of sewage outlet will have lots and gliding bacteria too. Should be lots at the bottom of any composting vegetable material, especially if undisturbed for several months or better still years.

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daruosha
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Re: Flagellated Bacteria

#9 Post by daruosha » Tue Apr 14, 2020 12:12 am

apochronaut wrote:
Mon Apr 13, 2020 10:50 pm
daruosha wrote:
Mon Apr 13, 2020 8:10 pm
The footage is certainly top notch. Never seen anything like that.

Is there any chance that I can find some flagellated bacteria too? I really want to observe it myself.
They seem to abound in oxygen depleted waters but are quite common in general. Many pathogenic bacteria sport flagella but in some cases they are not used for locomotion but for adherence.

Slow moving waters or those stalled due to dry weather should provide some. Dirty, scummy ponds. Anywhere close to any form of sewage outlet will have lots and gliding bacteria too. Should be lots at the bottom of any composting vegetable material, especially if undisturbed for several months or better still years.
Thanks for the tip. I'll try my luck.
Daruosh.

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KD Arvidsson
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Re: Flagellated Bacteria

#10 Post by KD Arvidsson » Tue Apr 14, 2020 12:20 pm

Very interesting and nice! //KD
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biptunia
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Re: Flagellated Bacteria

#11 Post by biptunia » Thu Apr 16, 2020 12:52 pm

Gorgeous shot of some wild and woolly critters.

What camera / adapter did you use?

Thanks!

sinabro
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Re: Flagellated Bacteria

#12 Post by sinabro » Thu Apr 16, 2020 4:50 pm

Thanks all...

apochronaut wrote:
Mon Apr 13, 2020 12:54 am
Do you have any idea what they were? What was the source of the water? In some of the water sources I collect from yearly, I see numbers of what would seem similar to me but I have difficulty imaging the flagella. The DIC and I presume an apo objective certainly brings out some wanting details . I have the apo but not DIC, as of yet.
Up until now I have simply blocked together the rod and thread bacteria as " gliding bacteria".

great video. It certainly points out that bacteria are not as bland and pointless to gaze at as some might suggest.
Hi apochronaut!

First up i'm bad at english
sorry.

If you are interested, try the following

Put a few slices of cabbage in the pond water and place it in a warm sunny place
By the time cabbage slices has changed color and sank into the water
Then Rub a piece of cabbage on a slide glass

Maybe you can identify two types of flagellated bacteria.



of course, i'm not 100% sure
Microscopy is just my hobby. Thanks...
biptunia wrote:
Thu Apr 16, 2020 12:52 pm
Gorgeous shot of some wild and woolly critters.

What camera / adapter did you use?

Thanks!
Hi biptunia!
My equipment is
Nikon d5300 dslr + Olympus bx50 microscope.
Thanks.

biptunia
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Re: Flagellated Bacteria

#13 Post by biptunia » Thu Apr 16, 2020 7:48 pm

Thank you.

How do you connect / adapt the DSLR to the microscope ?

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