Fragilaria

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Wes
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Fragilaria

#1 Post by Wes » Wed May 13, 2020 5:03 pm

I found these diatoms in the brown scum thats covering submerged stones in a river. The scum is absolutely loaded with diatoms, strongly recommend checking it out for all the diatom enthusiasts out there.

Best regards
Wes


Image
CZJ Apo 40/0,95

Image
Zeiss Neofluar 40/0,75 Ph2

Image
Zeiss Neofluar 40/0,75 Ph2
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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75RR
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Re: Fragilaria

#2 Post by 75RR » Wed May 13, 2020 5:25 pm

Super images! Do like your CZJ Apo 40/0,95
Last edited by 75RR on Wed May 13, 2020 5:26 pm, edited 1 time in total.
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DrPhoxinus
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Re: Fragilaria

#3 Post by DrPhoxinus » Wed May 13, 2020 5:26 pm

Great photos.

Where is the stream and what do you think was the water temp?

Gerard

Sabatini
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Re: Fragilaria

#4 Post by Sabatini » Wed May 13, 2020 5:29 pm

Very good work.

Thank you

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Wes
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Re: Fragilaria

#5 Post by Wes » Wed May 13, 2020 5:49 pm

Thank you all for the kind words.
DrPhoxinus wrote:
Wed May 13, 2020 5:26 pm
Where is the stream and what do you think was the water temp?
That would be the Danube river in Central Europe, the temperature was probably around 15-20˚ C. I think in general any river will do, just look for the brown slime thats covering submerged objects. Diatom diversity serves as a bioindicator for how polluted the source is, the more pollution the less diversity but not necessarily less diatom density.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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Hobbyst46
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Re: Fragilaria

#6 Post by Hobbyst46 » Wed May 13, 2020 6:21 pm

Wes wrote:
Wed May 13, 2020 5:49 pm
...
Excellent DIC and phase contrast images.
Are the phase contrast images post-processed ?

DrPhoxinus
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Re: Fragilaria

#7 Post by DrPhoxinus » Wed May 13, 2020 6:34 pm

Thanks for the information Wes,

Years ago I worked on the karyotype of the European Mud Minnow, Umbra krameri, endemic to the Danube and Dniester river drainages. I think it is threatened now. It has 44 chromosomes and the North American Umbra limi has 22 metacentrics, probably Robertsonian fusions.

Banarescu P, Taisescu E, Joswiak GR (1983) The karyotype of Umbra krameri (Pisces: Umbridae) in relation to other members of the suborder Esocoidei.

Now microscopes are a hobby.

Gerard

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Wes
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Re: Fragilaria

#8 Post by Wes » Wed May 13, 2020 6:42 pm

Hobbyst46 wrote:
Wed May 13, 2020 6:21 pm
Wes wrote:
Wed May 13, 2020 5:49 pm
...
Excellent DIC and phase contrast images.
Are the phase contrast images post-processed ?
Thanks Hobbyst! The first phase contrast image was post-processed, I fixed the levels and gave it a tiny amount of unsharpening, tweaked the color curves a bit but the most significant modification was the clear background (its a separate solid color layer that reflects the averaged color of the natural background). The second phase contrast image was virtually untouched, only the levels were pushed by a tiny bit but nothing more (post-processing didn't make it look better).
DrPhoxinus wrote:
Wed May 13, 2020 6:34 pm
Thanks for the information Wes,

Years ago I worked on the karyotype of the European Mud Minnow, Umbra krameri, endemic to the Danube and Dniester river drainages. I think it is threatened now. It has 44 chromosomes and the North American Umbra limi has 22 metacentrics, probably Robertsonian fusions.

Banarescu P, Taisescu E, Joswiak GR (1983) The karyotype of Umbra krameri (Pisces: Umbridae) in relation to other members of the suborder Esocoidei.

Now microscopes are a hobby.

Gerard
Fascinating, does the European Mud Minnow have only telocentric chromosomes that fused into the metacentrics of Umbra limi? What is the evolutionary relationship of the two species?

Best regards
Wes
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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ImperatorRex
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Re: Fragilaria

#9 Post by ImperatorRex » Wed May 13, 2020 6:50 pm

75RR wrote:
Wed May 13, 2020 5:25 pm
Super images! Do like your CZJ Apo 40/0,95
I agree but the Zeiss Neofluar 40/0,75 Ph2 is also not too bad ;-)
Great Photos Wes!

Hobbyst46
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Re: Fragilaria

#10 Post by Hobbyst46 » Wed May 13, 2020 6:52 pm

Thanks.
Indeed, this morning I scraped (with a toothbrush) a stone from a tiny temporary stream and found diatom treasures - hopefully will not disintegrate upon cleaning !
The 40X/0.75 Neofluar Ph2 is my bread and butter tool.

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Wes
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Re: Fragilaria

#11 Post by Wes » Wed May 13, 2020 7:24 pm

ImperatorRex wrote:
Wed May 13, 2020 6:50 pm
75RR wrote:
Wed May 13, 2020 5:25 pm
Super images! Do like your CZJ Apo 40/0,95
I agree but the Zeiss Neofluar 40/0,75 Ph2 is also not too bad ;-)
Great Photos Wes!
Thanks Jochen. Its the only phase contrast objective I have and it gives nice results. Would be interesting to compare to higher end 40x phase contrasts objectives one day.
Hobbyst46 wrote:
Wed May 13, 2020 6:52 pm
Thanks.
Indeed, this morning I scraped (with a toothbrush) a stone from a tiny temporary stream and found diatom treasures - hopefully will not disintegrate upon cleaning !
The 40X/0.75 Neofluar Ph2 is my bread and butter tool.
I was thinking of planting a flat piece of smooth glass and going back to it some weeks later to scrape off the slime layer with a credit card. If it doesn't capture too many sand particles it could be a great source for permanent slides.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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KD Arvidsson
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Re: Fragilaria

#12 Post by KD Arvidsson » Wed May 13, 2020 8:44 pm

Woow i must use superimages me too :D Congratulation for beautiful photo :D //KD
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RobBerdan
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Re: Fragilaria

#13 Post by RobBerdan » Thu May 14, 2020 2:21 am

Very high quality images - nice job!
RB

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Wes
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Re: Fragilaria

#14 Post by Wes » Thu May 14, 2020 4:52 pm

Thank you KD and RobBerdan!
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
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