Arcella sp., Two different views from 1 stack
Arcella sp., Two different views from 1 stack
Here is an Arcella sp. (Perhaps Arcella megastoma). I had a sample that contained quite a few of these so I spent time looking and collecting ones that had the most transparent shell. I photographed a "full stack" of images.
For the first image I performed stacking operations that emphasized and registered the outer surface. For the second image I made use of the very shallow DOF and ran only a part of the stack that allowed me to see inside the test ("optical sectioning").
We had a discussion going on in this post:
viewtopic.php?f=6&t=9534
So I thought I would add a single image from the middle of this Arcella image stack to emphasize (to me anyway) the value and versatility of image stacking when appropriate and possible.
For the first image I performed stacking operations that emphasized and registered the outer surface. For the second image I made use of the very shallow DOF and ran only a part of the stack that allowed me to see inside the test ("optical sectioning").
We had a discussion going on in this post:
viewtopic.php?f=6&t=9534
So I thought I would add a single image from the middle of this Arcella image stack to emphasize (to me anyway) the value and versatility of image stacking when appropriate and possible.
Re: Arcella sp., Two different views from 1 stack
Nice demonstration! The ability to peer into things is definitely the silver lining to a shallow depth of field.
Last edited by 75RR on Wed Jun 03, 2020 4:14 am, edited 1 time in total.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Arcella sp., Two different views from 1 stack
Funny how one can look and not see. Just noticed that it has 12 evenly spaced and evenly sized nuclei.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
-
- Posts: 760
- Joined: Thu Aug 23, 2018 10:12 pm
- Location: Lund, Sweden
Re: Arcella sp., Two different views from 1 stack
Indeed, one could make a clock out of it!
Re: Arcella sp., Two different views from 1 stack
Thank you Charles K , for sharing master microscopy imaging classes with us. Your images have been fascinating and enjoyable to me for years. Happy summer'20 to all
charlie guevara
charlie guevara
Re: Arcella sp., Two different views from 1 stack
I did notice that and found it interesting, According to Ferry's terrific site: https://www.arcella.nl/arcella-megastoma/ if it is Arcella megastoma it could have between 2-200 nuclei!
Now that's a great idea. I might have to make a real one like below. I suppose we could even call it a "Nuclear Clock"!
Re: Arcella sp., Two different views from 1 stack
Charlie, you have outdone yourself!
Cheers,
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
Kurt Maurer
League City, Texas
email: ngc704(at)gmail(dot)com
https://www.flickr.com/photos/67904872@ ... 912223623/
-
- Posts: 760
- Joined: Thu Aug 23, 2018 10:12 pm
- Location: Lund, Sweden
Re: Arcella sp., Two different views from 1 stack
Charles, the original is so beautiful, with its 12 natural hour-marks; the addition of hands and center degrades its beauty. Just my opinion.
Re: Arcella sp., Two different views from 1 stack
That is a thing of beauty. What type of magnification is used. Thanks
Re: Arcella sp., Two different views from 1 stack
Appreciate the comments. Thanks.
BTW... there were 43 images in the full stack.
This was shot with a 20/0.70 on my Olympus BHS. I used a 2.5X NFK photoeyepiece, and the DIC component adds another 1.25X. So the total magnification on the camera sensor was 62.5X. That puts the diameter of the subject at about 250 microns.
BTW... there were 43 images in the full stack.
- KD Arvidsson
- Posts: 368
- Joined: Wed Feb 12, 2020 6:47 pm
- Location: Sweden
Re: Arcella sp., Two different views from 1 stack
Wooow it is so beatifully photos and very fynny //KD
Microscope Nikon Labophot 2
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw
Panasonic GH4 and HY-2307 Camera+Euromex adapter.
Westcoast of Sweden.
https://www.youtube.com/channel/UCjsgbq ... dyl2x0Atpw