Arcella sp., Two different views from 1 stack

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c-krebs
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Arcella sp., Two different views from 1 stack

#1 Post by c-krebs » Tue Jun 02, 2020 12:34 am

Here is an Arcella sp. (Perhaps Arcella megastoma). I had a sample that contained quite a few of these so I spent time looking and collecting ones that had the most transparent shell. I photographed a "full stack" of images.
For the first image I performed stacking operations that emphasized and registered the outer surface. For the second image I made use of the very shallow DOF and ran only a part of the stack that allowed me to see inside the test ("optical sectioning").

Image

Image

We had a discussion going on in this post:
viewtopic.php?f=6&t=9534
So I thought I would add a single image from the middle of this Arcella image stack to emphasize (to me anyway) the value and versatility of image stacking when appropriate and possible.

Image

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75RR
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Re: Arcella sp., Two different views from 1 stack

#2 Post by 75RR » Wed Jun 03, 2020 3:56 am

Nice demonstration! The ability to peer into things is definitely the silver lining to a shallow depth of field.
Last edited by 75RR on Wed Jun 03, 2020 4:14 am, edited 1 time in total.
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MichaelG.
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Re: Arcella sp., Two different views from 1 stack

#3 Post by MichaelG. » Wed Jun 03, 2020 4:12 am

Beautifully done

MichaelG.
Too many 'projects'

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75RR
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Re: Arcella sp., Two different views from 1 stack

#4 Post by 75RR » Wed Jun 03, 2020 5:46 am

Funny how one can look and not see. Just noticed that it has 12 evenly spaced and evenly sized nuclei.
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viktor j nilsson
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Re: Arcella sp., Two different views from 1 stack

#5 Post by viktor j nilsson » Wed Jun 03, 2020 6:25 am

Indeed, one could make a clock out of it!

charlie g
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Re: Arcella sp., Two different views from 1 stack

#6 Post by charlie g » Wed Jun 03, 2020 3:37 pm

Thank you Charles K , for sharing master microscopy imaging classes with us. Your images have been fascinating and enjoyable to me for years. Happy summer'20 to all

charlie guevara

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c-krebs
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Re: Arcella sp., Two different views from 1 stack

#7 Post by c-krebs » Wed Jun 03, 2020 9:59 pm

75RR wrote:
Wed Jun 03, 2020 5:46 am
Funny how one can look and not see. Just noticed that it has 12 evenly spaced and evenly sized nuclei.
I did notice that and found it interesting, According to Ferry's terrific site: https://www.arcella.nl/arcella-megastoma/ if it is Arcella megastoma it could have between 2-200 nuclei!

viktor j nilsson wrote:
Wed Jun 03, 2020 6:25 am
Indeed, one could make a clock out of it!
Now that's a great idea. I might have to make a real one like below. I suppose we could even call it a "Nuclear Clock"!

Image

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KurtM
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Re: Arcella sp., Two different views from 1 stack

#8 Post by KurtM » Thu Jun 04, 2020 12:52 am

Charlie, you have outdone yourself! :lol:
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Re: Arcella sp., Two different views from 1 stack

#9 Post by viktor j nilsson » Thu Jun 04, 2020 5:49 am

Fabulous!

Hobbyst46
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Re: Arcella sp., Two different views from 1 stack

#10 Post by Hobbyst46 » Thu Jun 04, 2020 8:00 am

Charles, the original is so beautiful, with its 12 natural hour-marks; the addition of hands and center degrades its beauty. Just my opinion.

microbob3
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Re: Arcella sp., Two different views from 1 stack

#11 Post by microbob3 » Thu Jun 04, 2020 12:22 pm

That is a thing of beauty. What type of magnification is used. Thanks

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c-krebs
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Re: Arcella sp., Two different views from 1 stack

#12 Post by c-krebs » Fri Jun 05, 2020 11:49 pm

Appreciate the comments. Thanks.
microbob3 wrote:
Thu Jun 04, 2020 12:22 pm
That is a thing of beauty. What type of magnification is used. Thanks
This was shot with a 20/0.70 on my Olympus BHS. I used a 2.5X NFK photoeyepiece, and the DIC component adds another 1.25X. So the total magnification on the camera sensor was 62.5X. That puts the diameter of the subject at about 250 microns.
BTW... there were 43 images in the full stack.

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KD Arvidsson
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Re: Arcella sp., Two different views from 1 stack

#13 Post by KD Arvidsson » Wed Jun 17, 2020 8:11 pm

Wooow it is so beatifully photos and very fynny :D //KD
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