Beautiful - STEM OF COTTON PLANT 40X

Write test posts here. This is the place to experiment with the forum. I may delete the contents from time to time.
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Jonnyvine
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Joined: Tue Feb 06, 2018 3:55 pm
Location: London, UK

Beautiful - STEM OF COTTON PLANT 40X

#1 Post by Jonnyvine » Fri Mar 23, 2018 11:07 am

This cotton stem was taken from a prepared slide.

Looks very nice though. :mrgreen:

Specimen taken at 40X magnification.

Image

Please leave any comments on what you think... :)

MicroBob
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Location: Northern Germany

Re: Beautiful - STEM OF COTTON PLANT 40X

#2 Post by MicroBob » Fri Mar 23, 2018 5:10 pm

Thank you for showing this image!
Did you slice, stain and cover it yourself?
The image is perfectl sharp at the border, but somewhat unsharp in the middle. This is probably caused by your objectives not beeing planar types". You might improve this by stacking severals shots. I think you are using a smartphone? For some smartphones "open camera" app offers fixed focus photography. You would just change the fine focus a couple of µ for every shot.

The sharpness at the border is about perfect, a photoshop expert would probably clean the background and adjust the white point. So congratulation, nothing serious to complain! :D

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coominya
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Re: Beautiful - STEM OF COTTON PLANT 40X

#3 Post by coominya » Fri Mar 23, 2018 6:49 pm

Those dark holes are interesting. I assume they are used to transport nutrients in solution up the stem. They are surrounded by clusters of elongated cells that I suppose are there to strengthen the passageway and stop it collapsing?

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Jonnyvine
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Re: Beautiful - STEM OF COTTON PLANT 40X

#4 Post by Jonnyvine » Tue Mar 27, 2018 10:20 am

Thank you for your comment MicroBob and coominya, someone did recommend to stack them. :P

I'm amazing you guessed I took the picture from a smartphone MicroBob! :o

MicroBob
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Re: Beautiful - STEM OF COTTON PLANT 40X

#5 Post by MicroBob » Wed Mar 28, 2018 9:23 am

If you are good at guessing you don't have to know much! :lol:
I think you mentioned it somewhere that you use a phone.

Stacking requires a fixed camera focus and a fixed camera. If you can acheive this with your phone you could of cause use stacking.

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