Onion skin

Here you can post pictures and videos to show others.
Post Reply
Message
Author
User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Onion skin

#1 Post by gekko » Sun Jan 04, 2015 1:53 am

These are a result of my attempt at getting images from onion skin. I also tried iodine tincture staining, but I was not successful. All images were taken with the 20x objective.

1. Cells (oblique illumination):
Image

2. Cells (cross-polarized light):
Image

3. Xylem (bright field):
Image

4. Xylem (cross-polarized light):
Image

User avatar
75RR
Posts: 7347
Joined: Sun Oct 12, 2014 2:34 am
Location: Estepona, Spain

Re: Onion skin

#2 Post by 75RR » Sun Jan 04, 2015 10:02 am

Like number 3. Xylem. Works as a record and as a photograph.
I am tempted to have a go.
No microtome, do have a surgical blade though.
Any tips?

Have jumped the gun. See onion. :)
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

User avatar
vasselle
Posts: 2763
Joined: Sat Oct 25, 2014 5:32 pm
Location: France

Re: Onion skin

#3 Post by vasselle » Sun Jan 04, 2015 3:39 pm

Bonjour Gekko
Un colorant qui fonctionne bien et le bleu de méthylène
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Onion skin

#4 Post by gekko » Sun Jan 04, 2015 5:18 pm

75RR wrote:Like number 3. Xylem. Works as a record and as a photograph.
I am tempted to have a go.
No microtome, do have a surgical blade though.
Any tips?
Have jumped the gun. See onion. :)
Saw it: beautiful! As to microtomes, I merely peeled by hand a thin "skin" from the onion and then cut a roughly square piece a few mm to the side. No microtomes or surgical blades needed.

User avatar
gekko
Posts: 4701
Joined: Sun Oct 12, 2014 7:38 am
Location: Durham, NC, USA.

Re: Onion skin

#5 Post by gekko » Sun Jan 04, 2015 5:19 pm

vasselle wrote:Bonjour Gekko
Un colorant qui fonctionne bien et le bleu de méthylène
Cordialement seb
Many thanks, seb, for the suggestion.

Post Reply