Filamentous algae

[Seb28]

Obj.Nikon 20/0.50

autofluo

IMG_1228.jpg (89.41 KiB) Viewed 1736 times

fluo UV

IMG_2835.jpg (162.71 KiB) Viewed 1736 times

fluo B

IMG_2836.jpg (137.44 KiB) Viewed 1734 times

fluo UV+B

vbc.jpg (133.87 KiB) Viewed 1734 times

[75RR]

Love the effects of epifluorescence.
autofluo
fluo UV
fluo B
fluo UV + B

First one is the effect I am more familiar with.
How many flou's are there?

[Seb28]

Thank you 75RR

There are four fluorescent exitations: UV-ultra violet,V-fiolet,B-blue and G-green.You can also marge them together.

[gekko]

Wonderful images! By autofluo I guess you mean autofluorescence (before you add the fluo stain); is that right? What excitation wavelength do you use with autofluo?

[Seb28]

Thank you Gekko.
Yes,you're right.
Autofluorescence is the natural emission of light, you can get the autofluorescence on each set(UV,V,B,G) it all depends on the specimen you are watching,for example chlorophyll emit autoflurescence on B(blue excitation) filter set.
Mostly for autofluorescence i use:
UV:
-excitation filter 365/50nm
-dichroic mirror 395nm
-emission filter 445/50nm
B:
-excitation filter 546/10nm
-dichroic mirror 565nm
-emission filter 605/55

[Crater Eddie]

Shedding a new light on a favorite subject. Very interesting.
CE

[hkv]

Great images! I need to start using my fluorescence equipment as well. Never used it yet...

[vasselle]

Hello
Superbes séries images.
Cordialement seb