Drosophila melanogaster,fruit fly.
Drosophila melanogaster,fruit fly.
Drosophila melanogaster cleared in KOH,whole mount in glicerine.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Drosophila melanogaster,fruit fly.
Great, outstanding images, would be very interesting to know the technic you`re using
Regards
Manfred
Regards
Manfred
Re: Drosophila melanogaster,fruit fly.
Outstanding! Fluorescence?
Flickr: https://www.flickr.com/photos/micromundus
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Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Instagram: https://www.instagram.com/micromundusphotography
Web: https://hakankvarnstrom.com
Olympus BX51 | Olympus CX23 | Olympus SZ40 | Carl ZEISS EVO LS 10 Lab6 | Carl Zeiss Jena Sedival
Re: Drosophila melanogaster,fruit fly.
Wow, great work!
How did you mount such a thick insect, in glycerine, without crashing it, and prevent any subject movement after mounting? Sorry for the newbie question.
How did you mount such a thick insect, in glycerine, without crashing it, and prevent any subject movement after mounting? Sorry for the newbie question.
Re: Drosophila melanogaster,fruit fly.
I'll just repeat the others.
Fine work, both in terms of photography and as, zzffnn mentioned, in terms of mounting.
I have tried some insect/arthropod mounting and find it hit and miss.
Fine work, both in terms of photography and as, zzffnn mentioned, in terms of mounting.
I have tried some insect/arthropod mounting and find it hit and miss.
Re: Drosophila melanogaster,fruit fly.
Excellent images. Glad you did not just go with the easyzoom - much prefer the others.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Drosophila melanogaster,fruit fly.
Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.
Re: Drosophila melanogaster,fruit fly.
Thank you all for your kind comments.
I begin by placing the specimen in KOH/NaOH solution, heat up to ∼90–100 ◦C,5-10min, preferably in a water bath,then washing in pure water.
The most difficult procedure which is a placement and positioning the insect on the slide.
In the center of a microscope slide I put a drop of pure glycerine,transfer the insect by DIY spatula (bend syringe needle) in to glicerine drop. To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick. After properly positioning I put very gently the cover slip and sealed with nail varnish
.
I begin by placing the specimen in KOH/NaOH solution, heat up to ∼90–100 ◦C,5-10min, preferably in a water bath,then washing in pure water.
The most difficult procedure which is a placement and positioning the insect on the slide.
In the center of a microscope slide I put a drop of pure glycerine,transfer the insect by DIY spatula (bend syringe needle) in to glicerine drop. To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick. After properly positioning I put very gently the cover slip and sealed with nail varnish
.
102 images stack,Zeiss CP-achromat 10x/0.25,ring lamp,Canon 50Deinman wrote:Incredible images! I love the first one. How many stacks was the first image? What were the conditions? Lens etc.Significantly better than any I have produced to date.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Drosophila melanogaster,fruit fly.
This is not fluorescence,I invert bright field image in Photoshop,and play with the colors.hkv wrote:Outstanding! Fluorescence?
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Drosophila melanogaster,fruit fly.
Pretty neat tools!To positioning the insect under dissecting microscope I use DIY tools ,tattoo needle or cat / dog hair glued to toothpick.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Drosophila melanogaster,fruit fly.
Seb 28, these are all great images and your techniques are very good.
Re: Drosophila melanogaster,fruit fly.
No doubt those images places you into the world of a fruit fly. The time it takes to do all of this is worth the journey for most of us to view.
Rodney
Rodney
Re: Drosophila melanogaster,fruit fly.
These are exquisite... Very sharp and vibrant colors and clarity...
BillT
BillT
Re: Drosophila melanogaster,fruit fly.
Yes- Exquisite!
Re: Drosophila melanogaster,fruit fly.
Thank you all.
-Reichert Polyvar
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
-Olympus IX70
-Zeiss Photomicroscope
-Canon 600D
Re: Drosophila melanogaster,fruit fly.
Hi!
Thank you for such an inspiring subject!
I am wondering if you could help us (the newbies) in step-by-step preparation.
I find it hard to keep the sample in place. Or sometimes, the coverslips just crush the fly.
Thank you for such an inspiring subject!
I am wondering if you could help us (the newbies) in step-by-step preparation.
I find it hard to keep the sample in place. Or sometimes, the coverslips just crush the fly.