More diatoms
More diatoms
Polarizer and analyzer removed really brightens up the phase contrast.
Green Interference filter on field lens improves contrast and color.
Saturation and contrast enhanced in photoshop elements
7 image stack
20x Ph1
Olympus BX40
Brighter image. (Wow!)
8 image stack
background removed in PSE
My new avatar.
Radazz
Green Interference filter on field lens improves contrast and color.
Saturation and contrast enhanced in photoshop elements
7 image stack
20x Ph1
Olympus BX40
Brighter image. (Wow!)
8 image stack
background removed in PSE
My new avatar.
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: More diatoms
Those colors are gorgeous, well done!
What type of green interference filter are you using? I'm not very familiar with other than standard filters and I've never used an interference type.
What type of green interference filter are you using? I'm not very familiar with other than standard filters and I've never used an interference type.
Re: More diatoms
In agreement with both compliment and first question of GaryB, may I elaborate on the question.GaryB wrote:Those colors are gorgeous, well done!
What type of green interference filter are you using? I'm not very familiar with other than standard filters and I've never used an interference type.
For convenience, I will call 546nm "green", although it might be slightly different figure.
An ideal bandpass "green" interference filter can only transmit green, say wavelength of 545-546nm, and block all other wavelengths.
A non ideal interference filter will transmit a broader portion, say only those within 540-550nm, and cutoff all the rest.
That is a distinct advantage of interference filters over the cheaper absorption filters, those transmit a much broader portion of the spectrum, say within 500-600nm, and cutoff all the rest - below and above. Peak transmittance will be at 546nm, but significant transmittance will cover from 500 to 600nm (all numbers are suggested, according to my experience).
Your photo displays a very wide spectrum. Red and blue can be there only if they were included in the filtered light that hit the specimen.
Was it not a bandpass filter?
Or, were the colors added in software?
Re: More diatoms
I’m guessing it’s a GIF at all. Looking at it obliquely, it looks a bit like the coating on a dvd, while looking through into the light is a sort of milky green. I got it on the field lens of one of the Nikon labophot2 scopes I got from the medical school. Fit the Olympus perfectly, so I appropriated it.
As you see, it really enhances Phase contrast.
As I say, I’m guessing
Radazz
As you see, it really enhances Phase contrast.
As I say, I’m guessing
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: More diatoms
Wow, I just did a search on ebay and what you describe appears to be a GIF 550nm phase contrast. They seem to cost around $90 or so.
Olympus 45mm 550nm green phase contrast interference filter, Part # 43IF550W45
I'll need to see if there is a generic version for less.
https://m.ebay.com/itm/Olympus-45mm-Gre ... %7Ciid%3A1
Olympus 45mm 550nm green phase contrast interference filter, Part # 43IF550W45
I'll need to see if there is a generic version for less.
https://m.ebay.com/itm/Olympus-45mm-Gre ... %7Ciid%3A1
Re: More diatoms
That looks like the one. Mine is not in a frame, and looks like two pieces of glass with the coating between them.
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: More diatoms
Here’s one for a little less
https://www.ebay.com/itm/Olympus-Micros ... 3270665235
https://www.ebay.com/itm/Olympus-Micros ... 3270665235
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: More diatoms
I searched the web to find the transmission spectrum of the Olympus 43IF550-W45 filter but could not find it.
However, Olympus brochures specify it as a filter to produce monochromatic light, of wavelength 550nm, which is exactly the way to improve contrast in phase contrast microscopy. It almost certainly transmits narrow band, say 550+/- 10nm, that is, green light, without blue or red.
However, Olympus brochures specify it as a filter to produce monochromatic light, of wavelength 550nm, which is exactly the way to improve contrast in phase contrast microscopy. It almost certainly transmits narrow band, say 550+/- 10nm, that is, green light, without blue or red.
Re: More diatoms
Bonjour
Très belles images
Cordialement seb
Très belles images
Cordialement seb
Microscope Leitz Laborlux k
Boitier EOS 1200D + EOS 1100D
Boitier EOS 1200D + EOS 1100D