Paramecium

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Nanonaut
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Paramecium

#1 Post by Nanonaut » Mon Aug 13, 2018 1:40 pm

Hi all,

As my first post into the imaging section of this forum, I would like to share with you some of my recent shots of paramecium.

Images were captured with Canon 750D on an Olympus BX51 in DIC with a x20 objective.

Apologies for the low quality but the forum will not allow me to upload a file size exceeding 500kb.
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Paramecium formed small clusters in the vicinity of algal strands. This is one such cluster.
Paramecium formed small clusters in the vicinity of algal strands. This is one such cluster.
IMG_0049_1.jpg (474.93 KiB) Viewed 5965 times
Paramecium feeding on the biofilm that encapsulates a strand of alga. The cell nuclei and cytoskeletal network is visible in the algal cells that form the filament. It is mind blowing to watch the motor proteins streaming along the dynamic filaments that make up the network.
Paramecium feeding on the biofilm that encapsulates a strand of alga. The cell nuclei and cytoskeletal network is visible in the algal cells that form the filament. It is mind blowing to watch the motor proteins streaming along the dynamic filaments that make up the network.
IMG_0070.jpg (431.93 KiB) Viewed 5965 times
A rootlet of an aquatic plant provides the framework for a dense ecosystem of microscopic life. We can see it covered with all kinds of algal, cyanobacteria and diatomic species. A Paramecium bursaria feeds on bacteria living in this dense forest.
A rootlet of an aquatic plant provides the framework for a dense ecosystem of microscopic life. We can see it covered with all kinds of algal, cyanobacteria and diatomic species. A Paramecium bursaria feeds on bacteria living in this dense forest.
IMG_0236.jpg (382.6 KiB) Viewed 5966 times
Here we can see Paramecium bursaria photographed as it skimmed up and down along an algal strand feeding on bacteria. It's mouth part and cilia are clearly visible aswell as the mutualistic endosymbiotic Zoochlorella (green alga), birefringent crystals and various cellular compartments. The cellulose cell walls of the alga strand exhibiting optical activity.
Here we can see Paramecium bursaria photographed as it skimmed up and down along an algal strand feeding on bacteria. It's mouth part and cilia are clearly visible aswell as the mutualistic endosymbiotic Zoochlorella (green alga), birefringent crystals and various cellular compartments. The cellulose cell walls of the alga strand exhibiting optical activity.
DPP_0018.jpg (490.16 KiB) Viewed 5966 times
Last edited by Nanonaut on Mon Aug 13, 2018 8:47 pm, edited 2 times in total.
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billbillt
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Re: Paramecium

#2 Post by billbillt » Mon Aug 13, 2018 3:06 pm

Thanks for sharing.. Those are very good..

BillT
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Hobbyst46
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Re: Paramecium

#3 Post by Hobbyst46 » Mon Aug 13, 2018 3:24 pm

Great photos. If possible, please share the technical details - which optics, illumination etc. Thanks!
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ImperatorRex
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Re: Paramecium

#4 Post by ImperatorRex » Mon Aug 13, 2018 4:29 pm

Really great shots. Somehow reminds me to the old Zeiss DIC system - respectivley the color contrast image.
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Nanonaut
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Re: Paramecium

#5 Post by Nanonaut » Mon Aug 13, 2018 6:39 pm

Hobbyst46 wrote:Great photos. If possible, please share the technical details - which optics, illumination etc. Thanks!
Thanks, I have added in those details now.
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75RR
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Re: Paramecium

#6 Post by 75RR » Mon Aug 13, 2018 7:09 pm

Very nice. Especially like the second one.
Images were captured with Canon 750D on an Olympus BX51 in DIC at ~ x200 using flash.
I take it that means you used a 20x objective?
Could you tell/show us how the 'flash' is incorporated?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
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Radazz
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Re: Paramecium

#7 Post by Radazz » Mon Aug 13, 2018 10:36 pm

Very nice!
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Aenima
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Re: Paramecium

#8 Post by Aenima » Tue Aug 14, 2018 12:43 am

Stunning captures. :) Huge wow factor on that 2nd image.
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Captain_Howdy1
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Re: Paramecium

#9 Post by Captain_Howdy1 » Tue Aug 14, 2018 5:24 am

awesome pics dude
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charlie g
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Re: Paramecium

#10 Post by charlie g » Tue Aug 14, 2018 5:10 pm

Beautiful subjects, fantastic image captures, a sense of how these protozoa swarm and prod substrate, and tumble from you images...thank you for this wonderful posting. Charlie Guevara, I'm fond of protozoa.
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coominya
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Re: Paramecium

#11 Post by coominya » Thu Aug 16, 2018 12:17 am

"Bugger me (aussie expression) Those are exquisite photos nanonaut. Your composition is beautiful.
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vasselle
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Re: Paramecium

#12 Post by vasselle » Sun Aug 19, 2018 4:20 pm

Bonjour
Very nice images
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Nanonaut
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Re: Paramecium

#13 Post by Nanonaut » Sat Sep 28, 2019 11:22 am

Thanks a lot for all the nice comments everybody :D
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