Here you can post pictures and videos to show others.
-
hkv
- Posts: 971
- Joined: Sun Nov 02, 2014 8:57 pm
- Location: Sweden
-
Contact:
#1
Post
by hkv » Thu Oct 25, 2018 8:04 pm
Long time since I posted in this forum so I thought I would share a few images. I was out boating in august and noticed some small "algae" floating on the surface of the water. It was like small hairy balls and I took a sample in a beer can to check them out. To my surprise, they were not algae at all, but cyanobacterias. In fact, Gloeotrichia in colonial form. A very nice looking species, but hard to image due to all the hairs. Stacking is a pain due to artifacts in the final image which takes some manual work to remove. Some of these are stacked in several steps with the center in one go and the outer parts in a separate go. In the fluorescent images I have stained them with Acridine Orange for a few minutes. The inner part got less stain and remained green, whereas the outer hairs sucked up more of the staining fluid. Then rinsed in fresh water before imaging.
Click on the images to get more information about the technique and objectives used.
Darkfield:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
DIC:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
Brightfield:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
Fluo:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
Fluo:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
Fluo:
Cyanobacteria - Gloeotrichia by
Håkan Kvarnström, on Flickr
-
MichaelG.
- Posts: 2590
- Joined: Mon Apr 10, 2017 8:24 am
- Location: NorthWest England
#2
Post
by MichaelG. » Thu Oct 25, 2018 8:35 pm
Spectacular images
MichaelG.
Too many 'projects'
-
75RR
- Posts: 7593
- Joined: Sun Oct 12, 2014 2:34 am
- Location: Estepona, Spain
#3
Post
by 75RR » Thu Oct 25, 2018 9:08 pm
Agree! An excellent series.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
-
Hobbyst46
- Posts: 3120
- Joined: Mon Aug 21, 2017 9:02 pm
#4
Post
by Hobbyst46 » Thu Oct 25, 2018 9:21 pm
Great photos. To my taste the DF is nicest...
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo
-
KurtM
- Posts: 1618
- Joined: Tue Jun 09, 2015 12:08 am
- Location: League City, Texas
-
Contact:
#5
Post
by KurtM » Fri Oct 26, 2018 12:24 am
A pain to image, maybe, but the results are spectacular! Don't be a stranger, your images are always so very nice.
-
rnabholz
- Posts: 3081
- Joined: Thu Jan 08, 2015 10:11 pm
- Location: Iowa USA
-
Contact:
#6
Post
by rnabholz » Fri Oct 26, 2018 1:57 am
Your commitment to go the extra mile shows through in the spectacular results. Just beautiful, well done
Rod
-
hkv
- Posts: 971
- Joined: Sun Nov 02, 2014 8:57 pm
- Location: Sweden
-
Contact:
#7
Post
by hkv » Fri Oct 26, 2018 7:20 am
Thank you all for your comments! The darkfield image is also one of my favourite.
-
Radazz
- Posts: 982
- Joined: Sat Jun 18, 2016 11:55 am
- Location: Arnold, Missouri USA
#8
Post
by Radazz » Fri Oct 26, 2018 12:55 pm
Very beautiful images!
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
-
zzffnn
- Posts: 2714
- Joined: Sat Jun 20, 2015 3:57 am
- Location: Houston, Texas
-
Contact:
#9
Post
by zzffnn » Fri Oct 26, 2018 1:03 pm
Great images!
Advantage of fluorescence imaging can be clearly seen with this subject.
-
Grahame
- Posts: 99
- Joined: Fri Jun 29, 2018 7:42 am
- Location: New Zealand
#10
Post
by Grahame » Tue Oct 30, 2018 6:48 am
Neat images.
Have you tried a cross-eye stereo of any of them ?
-
hkv
- Posts: 971
- Joined: Sun Nov 02, 2014 8:57 pm
- Location: Sweden
-
Contact:
#11
Post
by hkv » Tue Oct 30, 2018 7:10 am
Grahame wrote:Neat images.
Have you tried a cross-eye stereo of any of them ?
No, I have not tried that. I have to struggle to see cross-eye stereo at all and mostly fail in doing so... Therefore, I never enable that feature when stacking. Would be neat to see these in 3D for sure.
-
MicroBob
- Posts: 2393
- Joined: Sun Dec 25, 2016 9:11 am
- Location: Northern Germany
#12
Post
by MicroBob » Tue Oct 30, 2018 7:40 am
Hi Håkan,
really great images! Every time I see how beautiful and detailed nature is it becomes more and more difficult to understand how badly we treat it.
It's nice of you to decribe the amount of work and capability that was neede to make these images. So we are not disappointed when we can't get anywhere near!
Bob
-
hkv
- Posts: 971
- Joined: Sun Nov 02, 2014 8:57 pm
- Location: Sweden
-
Contact:
#13
Post
by hkv » Tue Oct 30, 2018 9:04 am
MicroBob wrote:Hi Håkan,
really great images! Every time I see how beautiful and detailed nature is it becomes more and more difficult to understand how badly we treat it.
It's nice of you to decribe the amount of work and capability that was neede to make these images. So we are not disappointed when we can't get anywhere near!
Bob
Bob, thanks! I agree. It i amazing to see how beautiful nature is. Especially when looking close. Also fascinating to understand that protists together make up 2 times as much biomass as all animals in the world. If we include bacteria, the biomass is 40 times more than of animals. And these are hidden to the naked eye unless we make them visible using our instruments!
-
Aenima
- Posts: 214
- Joined: Sun May 07, 2017 12:01 am
- Location: sunny UK
#14
Post
by Aenima » Tue Oct 30, 2018 2:54 pm
wow - awesome shots - really like them
-
Tarloth
- Posts: 45
- Joined: Mon Sep 17, 2018 5:15 am
#16
Post
by Tarloth » Fri Nov 02, 2018 11:46 pm
Fantastic photographs!
-
RobBerdan
- Posts: 102
- Joined: Tue Nov 20, 2018 3:26 pm
- Location: Calgary
-
Contact:
#17
Post
by RobBerdan » Fri Nov 23, 2018 1:32 am
Beautiful photomicrographs well done.
-
hkv
- Posts: 971
- Joined: Sun Nov 02, 2014 8:57 pm
- Location: Sweden
-
Contact:
#18
Post
by hkv » Fri Nov 23, 2018 8:37 pm
Thanks! I appreciate your feedback!
