Ok, i got it, so the higher magnification of objectives i go then it is better to go with lower magnification of the eyepieces, so i keep the total magnification within limits, so 800X-1000X aren't really good unless i have good optics and using oil immersion?apochronaut wrote:Resolution of details in your subject. Yes you can see things moving and using eyepieces of a higher power increases the magnification but the fine details of what you are looking at get blurred. If you use an objective with higher magnification and a higher N.A., staying with a lower power eyepiece, you get more magnification and the finer details will be more defined and sharper.TareqPhoto wrote:My 40X objective is 0.65, and believe it or not, it allowed me to see something very tiny to move, it was so so difficult to see that with 4X or 10X objectives, and i did use 20X eyepieces.apochronaut wrote:You can only use the 20X eyepieces with the 10x objective. If your 40X is a .65 N.A. you enter the zone of what is called empty magnification, so even though everything gets bigger, it doesn't gain any resolution.....kind of like walking right up to a movie screen , hoping to see more detail.
You cannot go any more than 1000 x the N.A. of the objective for the magnification limit with any objective. You can use a 15X on most 40 X objectives , or if you happen to have a 40X with an N.A. of .85, you could get away with a 20X but I can't think that I have ever seen a high N.A. 40X on a 3 watt led microscope.
With higher resolution, you would be able to see some detail of bacteria at 400X but it is the objective creating that resolution and you just have to have really good ones.
What do you mean higher resolution? resolution of what?
Are there something like 40X eyepieces? so i can use that with 4X or 10x objectives if possible.
High power eyepieces also usually have poorer eye relief( the distance between your eye and the lens) and are often uncomfortable to use for long periods of time.
Based on the formula above a 40X .65 objective has a useful total magnification capability of about 650X, maybe 700X at the extreme, so using a 15X eyepiece with an average 40X objective is o.k. Anything above that will yield very little if any extra information.
A 4X objective, which usually have an N.A. of about .10 could accept a 25X eyepiece, although since you have a 10X objective ???? A 10X .25 objective could be used with a 25X eyepiece and that might have some value at 250X.
Most of the higher magnification eyepieces are special purpose and more often than not designed to be used with very high N.A. objective lenses.
So, when i see those videos of those germs or whatever you call it in details or can see the shapes at powers such as 100X or 400X so why can't i see those at same size even with 40X objectives and 20X eyepieces?
I think i saw amoeba and paramecium and something else, but all of them, all were very tiny so that i feel i don't know what they are, but in videos with lower magnifications they look really big or clear, not talking about fine quality but about the size, so i want to know things here before i really think if i buy very expensive microscope will make them suddenly bigger with same magnification, i just ordered adapters to connect cameras, so i don't know if they will be bigger or larger size with camera then by eyes.