Some better targets for HMC
Some better targets for HMC
OK, here are some better images.
Alanine 10x HMC
Safranine 10x HMC
OrangeG
These look way more textured than Oblique and POL to me, but my peepers are early 1950s vintage.
Still learning.
Radazz
Alanine 10x HMC
Safranine 10x HMC
OrangeG
These look way more textured than Oblique and POL to me, but my peepers are early 1950s vintage.
Still learning.
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
- ImperatorRex
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Re: Some better targets for HMC
Indeed great images Radazz!
Would be nice to have the comparison Oblique + Pol versus HMC.
Would be nice to have the comparison Oblique + Pol versus HMC.
Re: Some better targets for HMC
So jealous. Maybe I will take the time to make my own HMC slit plates.
mnmyco
mnmyco
Re: Some better targets for HMC
Interesting, something for the next session.ImperatorRex wrote:Indeed great images Radazz!
Would be nice to have the comparison Oblique + Pol versus HMC.
Thanks, Dominus,
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: Some better targets for HMC
Gorgeous images.
If you get a chance to compare alternative methods -- would be very interested to see them.
If you get a chance to compare alternative methods -- would be very interested to see them.
Re: Some better targets for HMC
Hi Radazz,
thank you for showing these nice images! I too would like to see a comparison of this rare contrast system with pol + oblique combined with pol.
I would also like to see an image of a typical phase contrast-object in phase and Hoffman contrast if possible.
The Hoffman system works with assymetrical stops - is there preferred way to orient them to the object and it's structures?
This might be a system that is uncommon but might as well have had more success.
Bob
thank you for showing these nice images! I too would like to see a comparison of this rare contrast system with pol + oblique combined with pol.
I would also like to see an image of a typical phase contrast-object in phase and Hoffman contrast if possible.
The Hoffman system works with assymetrical stops - is there preferred way to orient them to the object and it's structures?
This might be a system that is uncommon but might as well have had more success.
Bob
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Re: Some better targets for HMC
Nice to see this thread! I just picked up a Nikon 40x 0.55 HMC LWD objective with correction collar pretty cheap on eBay. Got three Nikon CF objectives for a grand sum of £75: the 40x HMC objective, a plan achromat 20x 0.40 ph2 phase objective with correction collar, and a simple E achromat 10x 0.25 ph1 objective. Worth a shot, I thought. The only phase objective I had previously was a 40x CF N plan objective (and a Heine condenser) plus some WI Lomo's, so I got two more phase objectives that will be useful to me, plus a completely new illumination technique!
I don't have a HMC condenser and I don't plan to get one as I'm using my Nikon objectives on a Wild M20 stand, but based on what I've read it seems like it should be possible to make a DIY version by making a slit anullus and placing it in an empty slot in my phase condenser. Getting the slit to line up with the modular plate in the objective might be tricky without being able to rotate it easily, but should be doable.
I'll post some results when I have some (but it might take a while before I have time to do that).
I don't have a HMC condenser and I don't plan to get one as I'm using my Nikon objectives on a Wild M20 stand, but based on what I've read it seems like it should be possible to make a DIY version by making a slit anullus and placing it in an empty slot in my phase condenser. Getting the slit to line up with the modular plate in the objective might be tricky without being able to rotate it easily, but should be doable.
I'll post some results when I have some (but it might take a while before I have time to do that).
Re: Some better targets for HMC
Hi viktor,
you might print a black disc with multiple slits with a laser printer on transparency film and get a better idea where the slit has to be.
I'm looking forward for your project updates.
Bob
you might print a black disc with multiple slits with a laser printer on transparency film and get a better idea where the slit has to be.
I'm looking forward for your project updates.
Bob
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Re: Some better targets for HMC
Hi Bob,
My idea was similar: to print a fine grid system on transparent film to see where the slit should be.
Looking at: https://micro.magnet.fsu.edu/primer/tec ... intro.html
The simplest design that could work is simply a single slit, as in figure 4. But I think it would be simple to add some polarizing film that covers half the slit and place a rotating polarizer below the condenser so as to manipulate the width of the slit, as in Figure 2.
Fun project, this.
My idea was similar: to print a fine grid system on transparent film to see where the slit should be.
Looking at: https://micro.magnet.fsu.edu/primer/tec ... intro.html
The simplest design that could work is simply a single slit, as in figure 4. But I think it would be simple to add some polarizing film that covers half the slit and place a rotating polarizer below the condenser so as to manipulate the width of the slit, as in Figure 2.
Fun project, this.
Re: Some better targets for HMC
Not enough room to set up both the HMC and Phase contrast scopes so I can't do a side by side of Ph and HMC, but I've got some PH shots to compare.MicroBob wrote:Hi Radazz,
thank you for showing these nice images! I too would like to see a comparison of this rare contrast system with pol + oblique combined with pol.
I would also like to see an image of a typical phase contrast-object in phase and Hoffman contrast if possible.
The Hoffman system works with assymetrical stops - is there preferred way to orient them to the object and it's structures?
This might be a system that is uncommon but might as well have had more success.
Bob
The HMC, POL, oblique set will be easy, its on the list.
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
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Re: Some better targets for HMC
Hi Radazz.
Can I ask you for a favour? Could you take a photo of the back focal plane of the HMC objective through your phase telescope/bertrand lens when you have set your scope up for HMC? I'd love to compare the look to my own DIY version.
best,
Viktor
Can I ask you for a favour? Could you take a photo of the back focal plane of the HMC objective through your phase telescope/bertrand lens when you have set your scope up for HMC? I'd love to compare the look to my own DIY version.
best,
Viktor
Re: Some better targets for HMC
Hi, Victor
Shot with my cell phone, so best of several tries.
The coils of the halogen illuminator filament are actually much more clear in the mark one eyeball.
Hope this is useful,
Radazz
Shot with my cell phone, so best of several tries.
The coils of the halogen illuminator filament are actually much more clear in the mark one eyeball.
Hope this is useful,
Radazz
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40
Re: Some better targets for HMC
Check out :
http://micro.magnet.fsu.edu/primer/tech ... intro.html
You can safely ignore the central modulator. You're not likely to find one.
Scroll down and take the Interactive Tutorial. You'll need Java installed. The tutorial is
not necessary but I've found it helpful.
Edmund Optics sells polarizing material for too much of course ($20 ?), but it's
probably the thinnest material you'll find and that's good.
Have fun.
Brad
http://micro.magnet.fsu.edu/primer/tech ... intro.html
You can safely ignore the central modulator. You're not likely to find one.
Scroll down and take the Interactive Tutorial. You'll need Java installed. The tutorial is
not necessary but I've found it helpful.
Edmund Optics sells polarizing material for too much of course ($20 ?), but it's
probably the thinnest material you'll find and that's good.
Have fun.
Brad
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- Joined: Thu Aug 23, 2018 10:12 pm
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Re: Some better targets for HMC
Many thanks, Radazz and Brad.Brad_ wrote:Check out :
http://micro.magnet.fsu.edu/primer/tech ... intro.html
You can safely ignore the central modulator. You're not likely to find one.
Scroll down and take the Interactive Tutorial. You'll need Java installed. The tutorial is
not necessary but I've found it helpful.
Edmund Optics sells polarizing material for too much of course ($20 ?), but it's
probably the thinnest material you'll find and that's good.
Have fun.
Brad
I've read that link many times. However, because it says that "Drawings of the modulator plates shown in Figure 2 are exaggerated and increased in size for the purposes of this discussion", I wanted to see how it looks like in a real-life example.
It's not perfectly clear from Radazz picture, but it helps.
This is what my DIY solution looks like:
Wild achr.apl. condenser with a slit cut from an old library card slid into the filter holder beneath the condenser.
I think the back focal plane looks similar to Radazz's. However, I think that your slit is a little longer? I'm going to try a couple of different lengths and widths to see how it impacts image quality.
I already have some polarizing film and tried to mask half of the slit as described in the link above, but I didn't really feel that it changed the effect as much as I'd hoped when I rotated another polarizer over the field lens. Will keep experimenting, though. Now that I have it working it's easy to make slight variations.
Radazz, have you tried the slit condenser with some other non-phase objectives? Mine gives really nice 3-dimensional oblique illumination with my other 20x and 40x objectives, and something similar to oblique dark field with my 10x. Very nice, actually! Much easier to switch between brightfield and HMC for me
Re: Some better targets for HMC
“Radazz, have you tried the slit condenser with some other non-phase objectives? Mine gives really nice 3-dimensional oblique illumination with my other 20x and 40x objectives, and something similar to oblique dark field with my 10x. Very nice, actually! Much easier to switch between brightfield and HMC for me”
I have, and I agree.
I have, and I agree.
Arnold, Missouri
Olympus IX70
Olympus BX40
Olympus SZ40
Olympus IX70
Olympus BX40
Olympus SZ40