Diatom ?

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75RR
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Diatom ?

#1 Post by 75RR » Tue Aug 20, 2019 9:54 am

120µm, Marine sample, Alboran Sea.
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Hobbyst46
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Re: Diatom ?

#2 Post by Hobbyst46 » Tue Aug 20, 2019 1:52 pm

If the question meant "is it?" - it looks like a diatom. They are quite large at the west side of the pool and their relatives/colleagues remain fairly small at the east side...

If the question meant "ID ?" - following the Licmophora experience, once this one sheds its green-golden robe, the view of the striae will aid identification :)

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Re: Diatom ?

#3 Post by 75RR » Tue Aug 20, 2019 3:30 pm

Thanks Hobbyst46, I suppose it was two questions in one.

Have not kept the slide so no future installment to the mystery from that source. Perhaps I will run into another at some point and get a little more detail.

I wonder if what we are seeing is the process of reproduction by binary fission.
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Francisco
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¿Re: diatomea?

#4 Post by Francisco » Tue Aug 20, 2019 6:15 pm

Possibly Amphora

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Re: Diatom ?

#5 Post by 75RR » Tue Aug 20, 2019 7:13 pm

Thanks Francisco, found this:

https://www.naturamediterraneo.com/foru ... _ID=220585

Not sure how much light it sheds on the reproduction question

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Re: Diatom ?

#6 Post by KurtM » Wed Aug 21, 2019 12:46 am

First thing I thought is Entomoneis.
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Re: Diatom ?

#7 Post by 75RR » Wed Aug 21, 2019 8:27 am

Thanks KurtM

your tip got me searching again and I think I found a closer suspect: Thalassiophysa hyalina

What do you think?

https://naturalhistory2.si.edu/smsfp/ir ... hyalin.htm

http://www.diatomloir.eu/Site%20Diatom/ ... astes.html

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Re: Diatom ?

#8 Post by KurtM » Thu Aug 22, 2019 12:46 am

Now there's one I'm totally unfamiliar with. Let's look into it and see if we can tag this guy. And you only found one? What size sample and from where - benthic, planktonic, or ? Or were you in the marina scraping mooring lines again? 8-) Do keep an eye out for others.
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Re: Diatom ?

#9 Post by 75RR » Thu Aug 22, 2019 5:48 am

According to algaebase - Thalassiophysa rhipidis Conger and Thalassiophysa hyalina (Greville) Paddock & P.A.Sims are currently accepted names. see links below

http://www.algaebase.org/search/genus/d ... s_id=44476

Conger: https://repository.si.edu/bitstream/han ... 14_1-8.pdf

Greville: https://ia802908.us.archive.org/7/items ... itlond.pdf
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Re: Diatom ?

#10 Post by 75RR » Sat Aug 24, 2019 6:41 am

Bumping this to see if I can get some confirmation on the ID
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Re: Diatom ?

#11 Post by Wes » Sat Aug 24, 2019 7:40 am

75RR wrote:Bumping this to see if I can get some confirmation on the ID
Sorry I have no clue, just wanted to say its a great quality shot as the rest of your diatom photos. Keep up the good work! This is taken with 40/0,65 plan DIC?
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Re: Diatom ?

#12 Post by 75RR » Sat Aug 24, 2019 8:54 am

Thanks Wes
This is taken with 40/0,65 plan DIC?
Yes, it was taken using the Plan 40x/0.65 DIC slider and the designated 40x/0.65 Plan Objective.

That 40x objective works surprisingly well for a 0.65

I would like at some point to try something with a higher NA but still dry.
Last edited by 75RR on Sun Aug 25, 2019 10:27 am, edited 1 time in total.
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Re: Diatom ?

#13 Post by Wes » Sat Aug 24, 2019 9:24 am

75RR wrote:Thanks Wes
This is taken with 40/0,65 plan DIC?
Yes it was. Works surprisingly well for a 0.65

I would like at some point to try something with a higher NA but still dry.
True, I remember some other photos you posted (of Licmophora I think) taken with the same lens and they look excellent in my opinion. Occasionally you can find the Zeiss Jena 40/0.95 Apo objective on the German ebay for well below 100 EUR and I personally really like this objective. It produces a surprisingly flat field and the image quality is quite satisfying. It would probably combine well with your existing DIC setup for the 40/0.65 objective.
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Re: Diatom ?

#14 Post by 75RR » Sat Aug 24, 2019 11:50 am

Zeiss Jena 40/0.95 Apo objective
Will put it on my wish list. Have you got a photo of yours?
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Re: Diatom ?

#15 Post by Hobbyst46 » Sat Aug 24, 2019 11:59 am

75RR wrote:
Zeiss Jena 40/0.95 Apo objective
Will put it on my wish list. Have you got a photo of yours?
PM sent.

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Re: Diatom ?

#16 Post by Wes » Sat Aug 24, 2019 2:21 pm

75RR wrote:
Zeiss Jena 40/0.95 Apo objective
Will put it on my wish list. Have you got a photo of yours?
Here is an image of it mounted on my Phomi.

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Re: Diatom ?

#17 Post by 75RR » Sat Aug 24, 2019 4:17 pm

Thanks Wes, just making sure what it looked like!

It is 45mm DIN standard right?
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Re: Diatom ?

#18 Post by Wes » Sun Aug 25, 2019 7:19 am

75RR wrote:It is 45mm DIN standard right?
Correct. It fits well on a turret with Zeiss West objectives. Combined adjustment of its coverslip correction collar, Optovar magnification and light color temperature/wavelength can yield remarkable detail for a dry objective.
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