Nitzschia sigma
Nitzschia sigma
Planapo 63x/1.4, DIC, 450µm length - 10µm width, stacked and stitched in Photoshop, Marine sample, Alboran Sea.
Crazy long diatom! Those of you with big screens will be able to see a little more detail
Left background as found - basically just cleaned up dust spots
Crazy long diatom! Those of you with big screens will be able to see a little more detail
Left background as found - basically just cleaned up dust spots
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Nitzschia sigma
If you right click on it you can open in a new screen and zoom.
- Crater Eddie
- Posts: 1858
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- Location: Illinois USA
Re: Nitzschia sigma
Stunning!
CE
CE
Olympus BH-2 / BHTU
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB
LOMO BIOLAM L-2-2
LOMO POLAM L-213 / BIOLAM L-211 hybrid
LOMO Multiscope (Biolam)
Cameras: Canon T3i, Olympus E-P1 MFT, Amscope 3mp USB
Re: Nitzschia sigma
That is very good!....
BillT
BillT
Re: Nitzschia sigma
Many thanks Crater Eddie, good tip Roldorf
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Nitzschia sigma
Beautiful color inside. Nice job!
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Nitzschia sigma
Hi Glen,
great image! The presentation with small picture here and a big one accessible via mouse click is nice. This was probably a great opportunity to use the 63/1.4 for its field of view and resolution.
This is probably one of the diatoms one usually finds only in fragments as it is so long and vulnerable. I always regret not having found the diatom in good shape.
Bob
great image! The presentation with small picture here and a big one accessible via mouse click is nice. This was probably a great opportunity to use the 63/1.4 for its field of view and resolution.
This is probably one of the diatoms one usually finds only in fragments as it is so long and vulnerable. I always regret not having found the diatom in good shape.
Bob
Re: Nitzschia sigma
Many thanks Wes and MicroBob
Perhaps there is a milder method that sacrifices speed but protects more delicate diatoms?
I can see how it would be difficult for such long diatoms to survive the cleaning process.This is probably one of the diatoms one usually finds only in fragments as it is so long and vulnerable. I always regret not having found the diatom in good shape.
Perhaps there is a milder method that sacrifices speed but protects more delicate diatoms?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
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Re: Nitzschia sigma
Another exquisite image!
According to that recently posted microbe paper on climate change, diatoms perform 25-40% of total primary production in the oceans. Impressive.
According to that recently posted microbe paper on climate change, diatoms perform 25-40% of total primary production in the oceans. Impressive.
Re: Nitzschia sigma
Thany thanks Sauerkraut
Light microscopy can provide more detail than we are used to getting, given that most microscopists tend to chose a magnification based simply on whether the diatom fits in the field of view.
I do not think that apart from the convenience of digital imaging (over film), we are exploiting it as much as we could. Stacking and stitching have the potential to transform microscope photography.
I am wondering, given that the fine detail in many diatoms is simply non-visible at medium magnifications - if the use of higher power and higher NA objectives via stitching should be the norm.MicroBob wrote:This was probably a great opportunity to use the 63/1.4 for its field of view and resolution.
Light microscopy can provide more detail than we are used to getting, given that most microscopists tend to chose a magnification based simply on whether the diatom fits in the field of view.
I do not think that apart from the convenience of digital imaging (over film), we are exploiting it as much as we could. Stacking and stitching have the potential to transform microscope photography.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Nitzschia sigma
That is true for sure! And this is not future technology but could be widely done with todays means. A 3D-Printer offers guides and movements in 3 axes and is available for about 250€. I think some people already have built up running systems and can imagine that they are available commercialy, e.g. for pathology, but in amateur microscopy the don't play a big role so far. One would only need one or two high power objectives, no expensive trinocular tube and manual interface.75RR wrote:Stacking and stitching have the potential to transform microscope photography.
On the other hand side it is the question what to do with these high resolution images: Hanging them on the wall? On all walls? Slideshow on the 4K TV? This may have been on factor that has limited the speed of development here. The other factor will have been that for the development you have to know about microscopy, mechanics, electronics and software and there are not so many people who can offer these skills in one person.
A StackStich machine based on a Steindorf Mikrobenjäger would be nice!
Bob
Re: Nitzschia sigma
Indeed! For those of you unfamiliar with this website's namesake (or is it the other way around) here is Charles' microbehunter:MicroBob wrote:A StackStich machine based on a Steindorf Mikrobenjäger would be nice!
http://www.microscopy-uk.org.uk/mag/art ... dorff.html
Last edited by 75RR on Wed Aug 28, 2019 10:55 am, edited 1 time in total.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Nitzschia sigma
By the way the left side to me appears somewhat less healthy compared to its right counterpart, namely referring to the structure and distribution of the brown pigment. Any idea on what dying diatoms look like?
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Nitzschia sigma
It does not take long to see a difference on the chloroplasts (a shrinking) when under the bright lights of a microscope. It may be an adjustment to the intensity of the light.By the way the left side to me appears somewhat less healthy compared to its right counterpart, namely referring to the structure and distribution of the brown pigment.
Don't think there is anything as dramatic to see as one does with ciliates which disintegrate.Any idea on what dying diatoms look like?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Nitzschia sigma
Wow so cool. I've seen a long diatom before but not that long.
I'm using a celestron microscope with 4, 10, 40, 100 times magnification. Eye pieces are 10 and 20.
My blog : https://www.facebook.com/MicroscopyHunter/
My blog : https://www.facebook.com/MicroscopyHunter/
Re: Nitzschia sigma
Thanks Chadack
I don't think I have either. Personal record I think!Wow so cool. I've seen a long diatom before but not that long.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
- ImperatorRex
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Re: Nitzschia sigma
Congrats 75RR - very well done
Re: Nitzschia sigma
Many thanks ImperatorRex, much appreciated
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)