Amphora sp. (probably Amphora arcus)
Amphora sp. (probably Amphora arcus)
Planapo 63x/1.4, DIC, 90 µm, stacked in Photoshop, Marine sample, Alboran Sea.
top = images 600-642 , bottom = images 635-672 and all = images 600-672
probably Amphora arcus (to be confirmed)
top = images 600-642 , bottom = images 635-672 and all = images 600-672
probably Amphora arcus (to be confirmed)
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- bottom.jpg (137.77 KiB) Viewed 4200 times
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- all.jpg (165.42 KiB) Viewed 4200 times
Last edited by 75RR on Fri Sep 20, 2019 2:27 pm, edited 1 time in total.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Amphora sp. (probably Amphora securicula)
Wow ! These images are amazing. I'm, curious how much time do images like this require ? Assuming one is proficient with the appropriate PS techniques ?
Dan
Dan
Re: Amphora sp. (probably Amphora securicula)
.
Note: I have amended the first post to reflect my new probable ID from Amphora securicula to Amphora arcus
I did not do the whole process in one go but if I had it would have taken a couple of hours.
The photographic session took about an hour from when I spotted the diatom and took the first images to when I went back to it later to see if it had slowed down enough to try again.
I could have done it in less if I had leached the water out rather than let it evaporate, but I was not sure what else was on the slide.
Post processing in Camara raw + photoshop took around an hour or so for the 3 images.
Note: I have amended the first post to reflect my new probable ID from Amphora securicula to Amphora arcus
Thanks mintakax,
I did not do the whole process in one go but if I had it would have taken a couple of hours.
The photographic session took about an hour from when I spotted the diatom and took the first images to when I went back to it later to see if it had slowed down enough to try again.
I could have done it in less if I had leached the water out rather than let it evaporate, but I was not sure what else was on the slide.
Post processing in Camara raw + photoshop took around an hour or so for the 3 images.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Amphora sp. (probably Amphora arcus)
Great images 75RR!
Do you know how big are the steps in your z-stack? I find that 1 µm (or smaller) vertical steps are ok for diatoms, otherwise I get artifacts in the striae stitching.
Do you know how big are the steps in your z-stack? I find that 1 µm (or smaller) vertical steps are ok for diatoms, otherwise I get artifacts in the striae stitching.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Amphora sp. (probably Amphora arcus)
Thanks Wes
A little less than one is probably about right.
Had a look just now at the amount of movement I get within the 2µm marks (microscope side on and reproducing the rotation feel) and reckon I get about 3 images per 2µm
On a slightly related issue, I have been disappointed with how little my new batteries seemed to last, until that is I read somewhere that they are supposed to give around 300 shots per charge!
So I stopped moaning, as it's not the battery - it's the fine focus ;)
A little less than one is probably about right.
Had a look just now at the amount of movement I get within the 2µm marks (microscope side on and reproducing the rotation feel) and reckon I get about 3 images per 2µm
On a slightly related issue, I have been disappointed with how little my new batteries seemed to last, until that is I read somewhere that they are supposed to give around 300 shots per charge!
So I stopped moaning, as it's not the battery - it's the fine focus ;)
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Amphora sp. (probably Amphora arcus)
How many miliampere hours is your new battery and how many is the old one? I hate it when there is some cool microbe or something exciting going on, then the battery dies and I have no replacement.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: Amphora sp. (probably Amphora arcus)
Note to self: Have to stop buying cheapest available!How many miliampere hours is your new battery and how many is the old one?
Murphy's Law. He visits me as wellI hate it when there is some cool microbe or something exciting going on, then the battery dies and I have no replacement.
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Amphora sp. (probably Amphora arcus)
Wes wrote:...
Folks, are there no AC adapters for Olympus cameras ? for my Canon I bought a (~20-30$ or even less, cannot remember) a 220V AC adapter. A Chinese thing. And I never touched the battery anymore, now for nearly 2 years.75RR wrote:...
Re: Amphora sp. (probably Amphora arcus)
Had a look at one point but did not find anything for the Olympus Ep-2Folks, are there no AC adapters for Olympus cameras ? for my Canon I bought a (~20-30$ or even less, cannot remember) a 220V AC adapter. A Chinese thing.
And I never touched the battery anymore, now for nearly 2 years.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: Amphora sp. (probably Amphora arcus)
I meant to make a thread asking exactly about this but now I know what I'm looking for. Thanks!
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel