euglenozoa
euglenozoa
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: euglenozoa
Very nice videos 75RR ! Both are detailed and interesting. Thanks.
Re: euglenozoa
Very fine details, really nice! You can clearly see the structure of the rod organ in Peranema. What other protozoans did you see in that sample?
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: euglenozoa
Thanks mintakax and Wes
I suppose since it is the end of summer it is concentrated.
Here are a couple more from the sample:
Quite a varied lot. Surprised me, was not expecting much.What other protozoans did you see in that sample?
I suppose since it is the end of summer it is concentrated.
Here are a couple more from the sample:
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: euglenozoa
Fantastic - thanks.
- ImperatorRex
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Re: euglenozoa
Very nice catches 75RR. How did you manage the oil immersion? There seems to be quite a thick liquid layer under the coverglas and this usually causes issues for such high n.a. objectives (low working distance) and oil immersion (movement or "pumping" of the coverglas due to re-focusing)? .
Re: euglenozoa
Many thanks PeteM and ImperatorRex
In this case that was not a problem as there was some large detritus that kept the cover slip from moving down, the downside of course was that the image quality suffered as several of the smaller subjects were not pressed up against the glass - the equivalent of having a thicker cover slip than recommended, which with the 63x/1.4 is very noticeable.How did you manage the oil immersion? There seems to be quite a thick liquid layer under the coverglas and this usually causes issues for such high n.a. objectives (low working distance) and oil immersion (movement or "pumping" of the coverglas due to re-focusing)? .
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: euglenozoa
Did you oil the top lens of the condenser?
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: euglenozoa
Yes I did. In fact I always do. In for a penny in for a pound.Did you oil the top lens of the condenser?
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
- ImperatorRex
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- Joined: Fri Mar 30, 2018 4:12 pm
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Re: euglenozoa
Thanks for the info 75RR.
I often use water instead of oil for the condensor front lense, just because of ease of cleaning. Works quite well till something like n.A. 1.2 or bit more.
I often use water instead of oil for the condensor front lense, just because of ease of cleaning. Works quite well till something like n.A. 1.2 or bit more.
Re: euglenozoa
Looking through the bertrand lens on the optovar unit I see an obvious difference upon oiling the condenser lens. Without oil I see a fairly limited field of illumination and following oiling the illuminated area increases dramatically. Now I wonder what difference there would be between using water, oil and other liquids with intermediate refractive index.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel
Re: euglenozoa
I would not recommend all of them but some might do at a pinch if you run out of immersion oil.Wes wrote: ↑Sat Sep 28, 2019 9:37 pmLooking through the bertrand lens on the optovar unit I see an obvious difference upon oiling the condenser lens. Without oil I see a fairly limited field of illumination and following oiling the illuminated area increases dramatically. Now I wonder what difference there would be between using water, oil and other liquids with intermediate refractive index.
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)
Olympus E-P2 (Micro Four Thirds Camera)
Re: euglenozoa
Nice list! I was thinking about glycerol, its very easy to clean as its water soluble and its refractive index is pretty close to the desired value.75RR wrote: ↑Sat Sep 28, 2019 10:15 pmI would not recommend all of them but some might do at a pinch if you run out of immersion oil.Wes wrote: ↑Sat Sep 28, 2019 9:37 pmLooking through the bertrand lens on the optovar unit I see an obvious difference upon oiling the condenser lens. Without oil I see a fairly limited field of illumination and following oiling the illuminated area increases dramatically. Now I wonder what difference there would be between using water, oil and other liquids with intermediate refractive index.
Zeiss Photomicroscope III BF/DF/Pol/Ph/DIC/FL/Jamin-Lebedeff
Youtube channel
Youtube channel