Pater noster tractor ruts meiofauna and protists

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charlie g
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Pater noster tractor ruts meiofauna and protists

#1 Post by charlie g » Thu Oct 17, 2019 5:13 pm

Hi all, it's been cool 40's-50's F, and rains too often for most here in finger-lakes/US. Finally on a clear day, doggie and I went for a microscopy collection hike. Our target the wetland drainage band between two deep sloped crop fields. We never made it..too distracted by 'pater noster trio of tractor ruts' in an ephemeral stream drain path headed from higher woodlands down the crop fields slope. And then there was that lone deer which ambled down the slope of the far cropfield to see what we were up to.

thanks for looking, Charlie guevara
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Re: Pater noster tractor ruts meiofauna and protists

#2 Post by charlie g » Thu Oct 17, 2019 5:30 pm

Err, that second image in my above post...an example of geographically proper: pater noster lakes. My microscopy world views have infused and enriched my world views. I used to see three tractor ruts with a gentle water flow..now I think: pater noster lakes, go figure. Yes that lone deer kept ambling down his sloped field to closer fancy our collection behaviors!

I elected to sample each ruts waters with separate collection jars..that deer kept approaching us.
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Re: Pater noster tractor ruts meiofauna and protists

#3 Post by charlie g » Thu Oct 17, 2019 5:48 pm

Back at my bench, the matt of algae from one tractor rut was tranfered to a shallow pyrex dish and left to settle a few hours. Please purchase low cost pyrex dishes..these hold shallow depth collection samples from which an oblique illumination from a LED torch permits scan of your sample for targets organisms to slup onto a microscope slide.
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Re: Pater noster tractor ruts meiofauna and protists

#4 Post by charlie g » Thu Oct 17, 2019 6:31 pm

Prior to this years chance tractor rutting, this specific area of vernal surface water flow down slope to the valley bottom drainage area was always a seep of less than one inch (? 2.53 cm?) depth...a near 'film of surface waters' which never hosted the field markers of rich meiofauna and protists. This is what distracted doggie and I to enjoy and collect from this err, pater noster trio of tractor ruts. This is a productive sloped crop field, upon next growth seasons plowing/ surface prep and planting...this aquatic community will dig back into the earth awaiting the next cycle of high water flows. Good doggie and I have encountered a fantastic example of the hardy and fantastic communities thriving in ephemeral yet long existing freshwaters, yes, yes!


Collection in second week of October, '19 contained the water flea cyclops with new egg baskets, and an interesting surface brown pigmented scale-encrustacion...no other epibionts noted.
Ostracod adult 'seed shrimp'/ water fleas...no epibionts noted on the dozen observed. And wonderful brown hydras...many with sexual organs developed on their body stalks...no epibionts noted on the six hydras I enjoyed observing. And for me the real treat of an active 'finger-nail clam'...this unique resident of ephemeral freshwaters, and permanent waters was an encounter which will have good doggie and I back to those gentle water flows to literally clam dig in a finger lakes crop field.

It was the finger-nail clam, aquatic insects, and a small snail which prompted me to use my rarely used: Nikon Plan 1X, 0.03, 160/ - objective, I DIY utilized this objective without removing my Nikon turrent 1.25 na phase/df/bf condenser...white tissue on the condenser upper lens-face, and a powerful hand held LED torch pointed down onto this white tissue...it works with no tedium of removing my properly centered phase turrent condenser.
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Re: Pater noster tractor ruts meiofauna and protists

#5 Post by 75RR » Thu Oct 17, 2019 6:44 pm

Missed your and your doggies adventures ...

will the Paternoster inundated ruts dry out or remain and freeze?
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Re: Pater noster tractor ruts meiofauna and protists

#6 Post by charlie g » Thu Oct 17, 2019 6:55 pm

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Sadly, numerous copepod water fleas were evident in scanning the sample in shallow pyrex dish, but these water fleas were quite agile in avoiding my fast-action collection eye-dropper. Technique is everything...I simply did not have the patience to disrupt the water sample by plodding after the water flea copepods like a diving king fisher bird. Once I captured this single cyclops with new egg-bags, I used methylcellulose viscosity tool to slow it down for my crude image-captures.

Please, pretty please look into use of methylcellulose for your microscopy observations of meiofauna and protists.

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Re: Pater noster tractor ruts meiofauna and protists

#7 Post by charlie g » Thu Oct 17, 2019 7:16 pm

Hi 75RR, this specific area of the deep sloped cropfield always seeps surface waters down to the drainage channel at bottom of this land...the bottom I guess is termed the 'valley floor'. In dead of winter with weeks/month or so of below freezing temps...there is liquid water under thick ice cover on the 'bottom land' drainage channel. I sense no flow down slope at these times, doggie and I hike these fields for years. This year totally wet/ rains to point of flood hazards in my area of Us...worse in other areas of US. Like with easy notice of things different for our Monarch butterflies, I really sense a change in weather patterns here, as elsewhere...go figure.

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Re: Pater noster tractor ruts meiofauna and protists

#8 Post by charlie g » Thu Oct 17, 2019 7:37 pm

Both these finger-nail clams and copepods...active in October'19 suggest these hardy meiofauna flourish/thrive year round if liquid waters are in their habitat. I have collected these same organisms under thick ice in dead of winter at this crop fields valley floor.

For it's wonderful to think that freshwater finger nail clams are now thought to have first evolved in con-US Missisippie river basin and then spread globally! I recall dear Anton L. long ago speculated in writings on the mechanism of dispersal of Duck Weed. Fresh water hydras also have now been designated as first appearing in one area of our dear globe...and then dispersing...mieofauna and protist dispersals, their niche habitats, all come to mind as doggie and I encountered this trio of pater noster tractor ruts.
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Re: Pater noster tractor ruts meiofauna and protists

#9 Post by charlie g » Thu Oct 17, 2019 9:06 pm

We are charmed by these brown hydras: Hydra oligactis species...Anton L. described this hydra species in Netherlands.
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Re: Pater noster tractor ruts meiofauna and protists

#10 Post by charlie g » Thu Oct 17, 2019 9:24 pm

Notable to me from circa 1950 textbooks, desmids indeed flourish on open moist soils...I enjoyed these brilliant crop field desmids, please enjoy these with me.

Ah yes, a large ciliate, 'new to me' encountered in these tractor ruts. Plenty of time, now that I imaged captured these ciliates, to 'look them up' in Kudos text..or online.

All in all it's such a treat to have literally stumbled upon these 'pater noster tractor ruts'...please keep your home areas spaces in mind for microscopy collection hikes. Thanks for looking at doggie and my most recent collection hike. Charlie Guevara finger lakes/US
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Re: Pater noster tractor ruts meiofauna and protists

#11 Post by 75RR » Fri Oct 18, 2019 4:30 am

...please keep your home areas spaces in mind for microscopy collection hikes.
If anyone required proof of the variety of life in seasonally wet areas - they have it now.

Must say the Hydra was a pleasant surprise and desmids are always a bonus.
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Re: Pater noster tractor ruts meiofauna and protists

#12 Post by charlie g » Thu Oct 31, 2019 9:59 pm

Hi all, the encounter with a finger-nail clam in the open field tractor ruts has good doggie and I back to collect mainly the mud sediments in those flooded field ruts.

My intent is to try and collect a few more of these dainty freshwater clams, to stock my artificial stream with these hardy molluscs.Image
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Re: Pater noster tractor ruts meiofauna and protists

#13 Post by charlie g » Thu Oct 31, 2019 10:11 pm

The hard part for me is to stop enjoying the microscopy views of these field samples, enchanting to see the high numbers of euglena protists, and those elegant desmids, the near monoculture of a Spirogyra species, and a specific type of amoeba...the tractor ruts are such a microscopy treat.

Like a film editor or book editor..I have to ruthlessly screen through these samples of sediment for the clams alone. The excluded water slurries I plop in a backwater area of my artificial stream.

Please enjoy images I intend to update my quest for finger nail-clams in this finger lakes/US thread. Charlie guevara
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Re: Pater noster tractor ruts meiofauna and protists

#14 Post by billbillt » Fri Nov 01, 2019 4:14 pm

A nice follow up on this interesting thread.. Please continue..

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Re: Pater noster tractor ruts meiofauna and protists

#15 Post by 75RR » Fri Nov 01, 2019 5:31 pm

My intent is to try and collect a few more of these dainty freshwater clams, to stock my artificial stream with these hardy molluscs
Can you give more details on your artificial stream and the role the freshwater clams will play in it?
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Re: Pater noster tractor ruts meiofauna and protists

#16 Post by charlie g » Sat Nov 02, 2019 9:58 pm

Hi 75RR, we moved to fingerlakes/NY from down state, had an unfenced (!!!!???) 38'x18'x10' deep outdoor inground pool located on a hill next to our mature/unthined/unmanaged wood lot on our new home. I converted this pool to a pond stocked with native fish/native plantings, I channeled a stream adjacent to this ersatz pond, and of course I fenced this aquatic habitat.

My goal for the 'artificial stream' ( now quite an area mesocosm native habitat..this over four years a thriving aquatic refugia)..my goal is...gulp...establish native freshwater mussels in sections of the stream...a project often having me hum: 'Man from Lamancha/ to dream the impossible dream'.
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Re: Pater noster tractor ruts meiofauna and protists

#17 Post by charlie g » Sat Nov 02, 2019 10:15 pm

Ahhh, clearly with hind sight/ 20-20 vision...doggie and I should have hiked to the tractor ruts with a metal or plastic mesh-screen..and sifted bottom muds right there at the collection site...ahhh. I have mud splashes on my outdoor picnic table, I collected only eight ( 8) finger-nail clams for my efforts with good doggie. And it was very messy. trying to be neat.
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Re: Pater noster tractor ruts meiofauna and protists

#18 Post by charlie g » Sat Nov 02, 2019 10:27 pm

With bow hunting season now well underway , with gun season starting...I sense these eight (8) finger nail clams are all going into my artificial stream. I will return to the protists in our tractor-rut collection samples. Thanks for looking, Charlie Guevara, finger lakes/US
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Re: Pater noster tractor ruts meiofauna and protists

#19 Post by charlie g » Wed Nov 27, 2019 4:25 pm

These tractor rut collected Euglena species are loaded with food store paramylum bodies, it's 11/19..no show of flagelae, extreme writhing/ 'euglenoid motions' hamper in focus total organism images. The writhing, spiral motions, the waves of bulges progressing along the Euglena's body lengths a pleasing observation. Doggie and I grateful to have done this collection hike.

happy thanks giving to all, Charlie guevara
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Re: Pater noster tractor ruts meiofauna and protists

#20 Post by charlie g » Wed Nov 27, 2019 4:36 pm

The near mono-culture ( certainly fair to call it an algal bloom) of one species of Spirogyra, the notable sprinkle of one species of large desmid within the filamentous algal bloom such charming hues of deep green and then the slow writhing/undulating Euglena species with their brick-red eye spots...a wonderful habitat within which the other meiofauna and protists all offer encounters for microscopy at 1X up to my dry 100X objectives. I will note to image these pater-noster tractor ruts in dead of winter after hunting season wanes.

Thanks for looking at this microscopy collection hike. Charlie guevara
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Re: Pater noster tractor ruts meiofauna and protists

#21 Post by charlie g » Wed Nov 27, 2019 5:04 pm

This 1880 London, UK text is in a series of natural history books for children. A microscopy treat for all ages! Aspects of Oliver's world wide microscopy forum carry on the pursuits advocated by this 1880 gem of a text. Charlie Guevara, finger lakes/US
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Re: Pater noster tractor ruts meiofauna and protists

#22 Post by charlie g » Wed Nov 27, 2019 5:16 pm

Happy thanks giving day to all, Charlie guevara
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Re: Pater noster tractor ruts meiofauna and protists

#23 Post by 75RR » Wed Nov 27, 2019 9:14 pm

-
Your Pater noster tractor ruts just keep on giving - those still waters run deep!

Image
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Re: Pater noster tractor ruts meiofauna and protists

#24 Post by Malpi » Sat Nov 30, 2019 3:16 am

Gosh, a most fascinating read with lots of stuff for a beginner (me) to follow up on.
Thank you.
Where should I start ?
Perhaps towards the end, with this :- "This 1880 London, UK text is in a series of natural history books for children."
I love dipping into 'old' books, the language and style is a reflection of more leisurely times.
So I googled a bit and found a copy to download at archive.org
(Infact I found two, one has some missing pages and is not OCRed to text.)
(is it permitted to post links ?)

your ref to it being a children's book reminded me of one of my favourite childhood books of a similar vintage, (-the book, not us!- ) on my grandmother's bookshelf :-
"Through Magic Glasses, and other lectures" by Arabella B. Buckley
(also available on archive.org as well as on gutenberg.org)
which set me off on a lifelong journey through amateur astronomy and other physics things and professionally to electronics and spacecraft telemetry !
Image
as you can see it is still with me !


A delayed prolog, (prologue ? don't ask) aka other musings__________>
"Pater noster tractor(1*) ruts",, "meiofauna(2*) and protists(3*)"
1:-Ferguson tractors, later Massey Ferguson
I knew of British Ferguson tractors (1a*), in my youth, causing ruts; I never heard of Paternoster Tractors, nor their ruts, before ! :) :) :)

I did know of the religious connotations with rosary beads, so it was most interesting to learn (upon googling) of glacial Paternoster lakes., as strings of lakes connected by thin streams. It seems that there are some in Scotland, UK but not ? this far south in England, UK.
An old geography teacher did tell me that the ice-age glaciers stopped about at this hillside ridge that I now live upon, (or maybe the one just to the north of me !)

1a:-My son-in-law has acquired one, restored it and uses it regularly for haymaking and other small-holding duties, as well as showing it at 'gatherings' :)

This following started as a memo to myself (variously from google snippets or wiki), so cut and discard as needed, now musings of a beginner in biology and microscopy ,,,

2:- meiofauna,
(also Meiobenthos)
"are small benthic(4) invertebrates that live in both marine and fresh water environments. The term meiofauna loosely defines a group of organisms by their size, larger than microfauna but smaller than macrofauna, rather than a taxonomic grouping. In practice, that is organisms that can pass through a 1 mm mesh but will be retained by a 45um mesh, but the exact dimensions will vary from researcher to researcher."

{ 4:- benthic =
{ " Benthos is the community of organisms that live on, in, or near the seabed, river, lake, [ or stream bottom, also known as the benthic zone.[1] This community lives in or near [ marine or freshwater sedimentary environments, from tidal pools along the [ foreshore, out to the continental shelf, and then down to the abyssal depths. "
}
I already had a sort of an idea about benthic, as destinct from pelagic, but this was a good time to revise.

3:-
Protists " are a group of loosely connected, mostly(?3a*) unicellular eukaryotic(3b*) organisms that are not plants, animals or fungi. There is no single feature such as evolutionary history or morphology common to all these organisms and they are unofficially placed under a separate kingdom called Protista. Therefore, protists are no longer a formal classification, and different members show varying degrees of homology "
{gulp, this could take a wee while!}
" with species belonging to all five eukaryotic kingdoms. However, it is still used as a term of convenience to describe eukaryotic microscopic organisms. "
(gulp)
from https://biologydictionary.net/protist/

(3a) hmmm I wonder what the other, less than mostly, are 2? 3? multi ??
(3b)
Eeek, watch this space


"that lone deer which ambled down the slope"


Perhaps looking for a rut !? LOL

"microscopy world views have infused and enriched my world views."
And now mine too it seems ! Thank you, fascinating stuff :)

"Please purchase low cost pyrex dishes..these hold shallow depth collection samples"
and can be officially declared one's own, avoiding confusion with and the displeasure of the domestic staff :)

"pater noster trio"
a triumvirate even ?

"no epibionts noted on the dozen observed."
" An epibiont (from the Ancient Greek meaning 'living on top of') is an organism that lives on the surface of another living organism. An epibiont is, by definition(A*), harmless to its host. In this sense, the interaction between the two organisms can be considered neutralistic or commensalistic; as opposed to being, for example, parasitic, in which case one organism benefits at the expense of the other, or mutualistic, in which both organisms obtain some explicit benefit from their coexistence). Examples of common epibionts are barnacles, remoras, and algae, "
Wow! Never too old to learn -- I knew about parasites and symbionts,,, but there is a whole other world out there !!
(A) by who's definition ? More research needed! That sort of casual assumed knowledge reminds me of an old maths prof who had the very annoying habit of saying ",, it is obvious therefore that ,,, " grrrr, it wasn't always obvious !

"finger-nail clam"
Not met them before, come next spring I'll have to investigate if we have them in south-west GB !
"and then spread globally!"
moments later in a following post my prayer is answered, and no beads were involved !!

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Re: Pater noster tractor ruts meiofauna and protists

#25 Post by MichaelG. » Sat Nov 30, 2019 9:35 am

Great post, Malpi ... I enjoyed reading that.

Regarding your rhetorical question
" An epibiont (from the Ancient Greek meaning 'living on top of') is an organism that lives on the surface of another living organism. An epibiont is, by definition(A*), harmless to its host. […]
(A) by who's definition ?
I think that claim probably comes from the ‘epibiont manifesto’ and may need ‘fact-checking’
... Recent polls suggest that their hosts are unconvinced.

:D MichaelG.

.
https://www.hindawi.com/journals/jmb/2014/891943/
Too many 'projects'

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Re: Pater noster tractor ruts meiofauna and protists

#26 Post by Malpi » Sat Nov 30, 2019 1:20 pm

MichaelG. wrote:
Sat Nov 30, 2019 9:35 am
I think that claim probably comes from the ‘epibiont manifesto’ and may need ‘fact-checking’
... Recent polls suggest that their hosts are unconvinced.
https://www.hindawi.com/journals/jmb/2014/891943/
👍 :) Nice find thanks

I was a bit uncertain about making such a long post,
I hope I did not bore too many people too much !

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Re: Pater noster tractor ruts meiofauna and protists

#27 Post by redflanker » Sat Nov 30, 2019 1:24 pm

Nice place! where did you find the hydra? The depths of the water?
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Re: Pater noster tractor ruts meiofauna and protists

#28 Post by billbillt » Sun Dec 01, 2019 9:28 pm

PLEASE CONTINUE WITH THIS WONDERFUL THREAD!

THE BEST,
BILLT

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Re: Pater noster tractor ruts meiofauna and protists

#29 Post by charlie g » Sun Dec 01, 2019 9:29 pm

Thanks, Michael G./ Malpi for your wonderful sharing of a 'founding text for you as a child' : " Through Magic Glasses"/ Ms. Arabella B. Buckley. I am eager to encounter and read this suggested text you treated us to images of.

Thanks, M.G. for your pleasure with doggie and my recent microscopy field collection hike. Nature enjoyment/ natural history enrichment/ local area conservation of natural systems/ birding/fishing/ botanical stewardship/ fresh water biology/ environmental stewardship...and more...all are facets of microscopy we all can enjoy...where we reside...and the treat of collection samples from areas (land/lake/ or marine) we visit on holiday...it makes sense to me that microscopy world views are as natural as our understanding of the larger aspects of our gorgeous world experiences.

Google and purchase low-cost, or read online ( I prefer to have a cluttered library of info...I'm a dinosaur)..texts on pond life, freshwater biology, foodwebs and the protists and meiofauna.

Latter the paired texts ( the publisher intended these two tomes to be a pair) : " Ecology and Classification Of North American Freshwater Invertebrates" 2nd ed., Thorp and Covitch, Academic Press...and: "Freshwater Algae of Nporth America, Ecology and Ckassification", Wehr and Sheath....well for Charlie g (me) , on this side of the pond...these texts are delicious treats..err..when there is time after work and chores.

You splinter off to aspects...diatoms, desmids, protozoa, rotifers, hydras..etc… but the core is that these are indeed our neighbors whom perform services for our world..wether or not we know about their world...our world. Image captures permit great satisfaction of your encounters ( I am to date a visual amateur astronomer with refractor telescope...I only imaged Mercury transit of our Sol with a handheld camera to the eyepiece...same for a few comets).

Thanks for your keen enthusiasm, M.G, thanks for this wonderful post you placed in this thread!

Here is a 'founding text of mine as a child under age of 12 yrs: " The Private Life of the Protozoa", Winifred Duncan, 1950...a beautiful text which partners well with " How to Know the Protozoa" byJahn! The librarian had to give 'a special permission' to take out the Duncan book...as it was in the: "adult collections"...and I was a child library card holder.
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Re: Pater noster tractor ruts meiofauna and protists

#30 Post by charlie g » Sun Dec 01, 2019 9:35 pm

Hi, redflanker..the hydras by habit...feed at or near waters surface...in less than 2.53 cm deep ditch/ rut waters. But I crudely scooped my collection samples..so I did not know where these hydras were living. Hydras are quite mobile..so they perhaps go to different areas of shallow ditch-waters..at different times of day, different temps, different sunshine levels. Charlie guevara

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