I've been playing with a stereo microscope (20-60x) and have been amazed at what it is possible to see even at these magnifications.
I know stains are usually used for fixing slides for compound microscopes, but is there any reason they wouldn't work also with a stereo microscope?
I've been able to observe cells in fruit and veg, and organelles inside paramecia. It seems to me that it might be possible to see a lot more without having to resort to a compound scope just yet or go through the whole paraffin slide fixing process.
I'm thinking of trying a H&E stain, but I'm not wanting to spend ages prepping specimens, if that makes sense - I'm just a curious hobbyist.
Cheers
Markus
Any point getting stains with a stereo microscope?
Any point getting stains with a stereo microscope?
His wife thinks he does protist too much.
____________________________
Zeiss Universal
BMPCC6K
AM Stereo scope
____________________________
Zeiss Universal
BMPCC6K
AM Stereo scope
Re: Any point getting stains with a stereo microscope?
They certainly do add to hand sections, see here
See here too....
Suggest, safranin (powder to mix yourself), alcian-blue - both aqueous, both cheap and available as powders!
See here too....
Suggest, safranin (powder to mix yourself), alcian-blue - both aqueous, both cheap and available as powders!
John B
Re: Any point getting stains with a stereo microscope?
Thanks John, those threads were great! Fantastic detail! It's nice to see the kind of results you can get with hand-sectioning.
Would you say those stains are a better bet to start off with than Eyosin and Hematoxalin?
Cheers
Markus
Would you say those stains are a better bet to start off with than Eyosin and Hematoxalin?
Cheers
Markus
His wife thinks he does protist too much.
____________________________
Zeiss Universal
BMPCC6K
AM Stereo scope
____________________________
Zeiss Universal
BMPCC6K
AM Stereo scope
Re: Any point getting stains with a stereo microscope?
Without a shadow of doubt yes - Haematoxylin (I use Harris for nuclear staining as it also acts very well as a general, and single stain, for my mounted sections) is very good for Botanicals but it's alcohol/water carrier/solvent as used (rather than the purely aqueous of safranin and alcian-blue) is an extra hassle with live/fresh hand sections which don't really shine in alcohol. Personally I find eosin to be of little use for Botanicals - just never seems to 'take' strongly enough even though it's a plasma-stain at heart.
Safranin and alcian-blue are superb together (applied serially or even as a combination or 'bi-stain') as nuclear/lignin and plasma/cellulose stain and counter-stains respectively. The alcian-blue really has no interest in those tissue/cell-components that are most attractive to safranin and the two differentiate quite nicely - very nicely in fact. I think you'll find eosin/haematoxylin a bit of a chore, speaking in the context of botanical subjects that is....
Get cutting, you'll find it's far easier than you imagine!
John B
Re: Any point getting stains with a stereo microscope?
Hi Markus,
your microscope will give you an nice overview of botanical sections and even show a bit smaller details. Differentiating staining makes it easier to identify different functional structures and also offer a more attractive view. I like Etzold FCA stain and Wacker W3A stain. You will find examples when searching for these stains.
Bob
your microscope will give you an nice overview of botanical sections and even show a bit smaller details. Differentiating staining makes it easier to identify different functional structures and also offer a more attractive view. I like Etzold FCA stain and Wacker W3A stain. You will find examples when searching for these stains.
Bob
-
- Posts: 6325
- Joined: Fri May 15, 2015 12:15 am
Re: Any point getting stains with a stereo microscope?
The last one I bought was covered in stains but I eventually got most of them off!