Leukocyte crawling in blood
Leukocyte crawling in blood
Sped up 4x, 40x/0.95 DIC with 2x optovar magnification.
Maybe I can add some bacteria or yeast to the blood and see if they react to it.
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Re: Leukocyte crawling in blood
Super video!
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Re: Leukocyte crawling in blood
Creepy enough for a monster movie!
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Re: Leukocyte crawling in blood
Beautifull!!
Re: Leukocyte crawling in blood
Very, very interesting indeed to see that. Thanks for sharing.
Tom W.
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Re: Leukocyte crawling in blood
That is awesome!!
Re: Leukocyte crawling in blood
Thank you all for the interest and comments.
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Re: Leukocyte crawling in blood
That's a white blood cell right? Then it's not as creepy as I thought.
Re: Leukocyte crawling in blood
Yes, probably a neutrophil. Someone asked me in the youtube comments section what are the small fast moving things. Someone else suggested platelets, initially I thought it may be but then I checked again and platelets were not as numerous and small as the particles so any further suggestions are welcome.DonSchaeffer wrote: ↑Mon Oct 19, 2020 10:19 pmThat's a white blood cell right? Then it's not as creepy as I thought.
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Re: Leukocyte crawling in blood
Wes,
impressive video for certain. When I saw the Leukocyte I first though it looks like an amobea? And the small little things moving arround would be just bacteria?
But this would then mean that the blood sample got contaminated with an other sample. Just thoughts and I have really to admit that I have no idea how Leukocytes actually look like.
impressive video for certain. When I saw the Leukocyte I first though it looks like an amobea? And the small little things moving arround would be just bacteria?
But this would then mean that the blood sample got contaminated with an other sample. Just thoughts and I have really to admit that I have no idea how Leukocytes actually look like.
Re: Leukocyte crawling in blood
Thank you Jochen1
In phase contrast I could observe extremely fine fibers, I think made from the protein fibrin that clots blood.
This was a fresh blood sample from my finger. If these were bacteria I would've been very sick at this point and I felt better than ever I suspect its dirt from the slide/cover slip although I don't see this in pond samples but I've seen the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.ImperatorRex wrote: ↑Tue Oct 20, 2020 5:04 pmAnd the small little things moving arround would be just bacteria?
In phase contrast I could observe extremely fine fibers, I think made from the protein fibrin that clots blood.
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Re: Leukocyte crawling in blood
Wes,
how did you actually prepare the slide? Wonder if you added other ingredience, for example an isotonic saline solution? Maybe this was contaminated over the time?
the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.
This is really interesting!
how did you actually prepare the slide? Wonder if you added other ingredience, for example an isotonic saline solution? Maybe this was contaminated over the time?
the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.
This is really interesting!
Re: Leukocyte crawling in blood
I took a needle, pricked my finger and deposited a small drop of blood on a coverslip that I then added to a slide. No isotonic saline, as simple as you can picture it.ImperatorRex wrote: ↑Wed Oct 21, 2020 4:35 pmWes,
how did you actually prepare the slide? Wonder if you added other ingredience, for example an isotonic saline solution? Maybe this was contaminated over the time?
the little particles in other videos of fresh blood... so a blood specific thing, suggestions are welcome.
This is really interesting!
I just checked a bunch of DIC blood videos on youtube and I see the exact same particles. So I say blood specific thing...maybe platelet particles/chunks or cellular debri?
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Re: Leukocyte crawling in blood
In a very real sense, leukocytes are amoebae. Cells that use amoeboid (myosin-mediated) movement are quite common among animals, and the ancestor we share with our nearest single-celled relatives (choanoflagellates, filastereans, etc.) likely had an amoeboid life phase, as well: https://bmcbiol.biomedcentral.com/artic ... 020-0762-1. On the eukaryotic tree of life, we happen to share a large branch with Amoebozoa--a broad clade called Amorphea, made up of amoebozoans, animals, fungi and some others. So, it's not too surprising that we share some cellular phenotypes with our little amoeba-cousins.ImperatorRex wrote: ↑Tue Oct 20, 2020 5:04 pmWhen I saw the Leukocyte I first though it looks like an amobea?
The video is just stunning, Wes!
Re: Leukocyte crawling in blood
Thank you Bruce for the comments and interesting paper that you linked reminding us of our distant relativesBruce Taylor wrote: ↑Wed Oct 21, 2020 9:48 pmIn a very real sense, leukocytes are amoebae. Cells that use amoeboid (myosin-mediated) movement are quite common among animals, and the ancestor we share with our nearest single-celled relatives (choanoflagellates, filastereans, etc.) likely had an amoeboid life phase, as well: https://bmcbiol.biomedcentral.com/artic ... 020-0762-1. On the eukaryotic tree of life, we happen to share a large branch with Amoebozoa--a broad clade called Amorphea, made up of amoebozoans, animals, fungi and some others. So, it's not too surprising that we share some cellular phenotypes with our little amoeba-cousins.ImperatorRex wrote: ↑Tue Oct 20, 2020 5:04 pmWhen I saw the Leukocyte I first though it looks like an amobea?
The video is just stunning, Wes!
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Re: Leukocyte crawling in blood
Not so distant, in the scheme of things. Here's the eukaryote tree, from a recent classification:
Image created by Yana Eglit for: https://onlinelibrary.wiley.com/doi/ful ... /jeu.12691
We're in Holozoa at the upper right, cuddled up to Nucletmycea (Fungi, Nuclearia, etc.), just downstream from the branching of Amoebozoa.
(Of course, this only includes complex cells; most of the world's diversity consists of bacteria and archaea)
Re: Leukocyte crawling in blood
Thank you Bruce you for helping me develop a better appreciation of our evidently not-so-distant amoeboid cousins. Now I feel humbled when an amoeba crosses the field of viewBruce Taylor wrote: ↑Thu Oct 22, 2020 3:24 pmNot so distant, in the scheme of things. Here's the eukaryote tree, from a recent classification:
Euk tree.png
Image created by Yana Eglit for: https://onlinelibrary.wiley.com/doi/ful ... /jeu.12691
We're in Holozoa at the upper right, cuddled up to Nucletmycea (Fungi, Nuclearia, etc.), just downstream from the branching of Amoebozoa.
(Of course, this only includes complex cells; most of the world's diversity consists of bacteria and archaea)
Very kind of you westy, thanks!
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Re: Leukocyte crawling in blood
An old but very informative video that matches this topic and content:
https://av.tib.eu/media/18700
https://av.tib.eu/media/18700
Re: Leukocyte crawling in blood
Thanks! There are also some very interesting videos of Vampyrella eating the chloroplasts of Spirogyra on this site.ImperatorRex wrote: ↑Sat Nov 07, 2020 9:10 amAn old but very informative video that matches this topic and content:
https://av.tib.eu/media/18700
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