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PostPosted: Mon Jun 11, 2018 7:50 am 
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Joined: Wed Mar 11, 2015 4:35 am
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Diatom Sample at 640X (40X Objective x 16X Eyepieces)
Single shot, Crop and Adjust Contrast / Brightness

Diatom was wet mount, but the surrounding water was dry up, some small drop can be seen in the photo.

The 40X Objective work well for Oblique Illumination.
The 16X Eyepieces magnify a little more than 10X Eyepieces, so small details can be seen easier.



Suphot


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640X Sample.jpg
640X Sample.jpg [ 100.45 KiB | Viewed 248 times ]
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PostPosted: Mon Jun 11, 2018 12:31 pm 
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Nice detail in that image!
Did you get a chance to measure it?
One of the nice things about oblique is that it lets you use most if not all of the NA of the objective.

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PostPosted: Mon Jun 11, 2018 3:57 pm 
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Great image!... Oblique attempts to mimic DIC, and this one approaches that.. It does not have the perfectly crisp outline that makes DIC shine, but great pic... Thanks for sharing...


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PostPosted: Thu Jun 14, 2018 6:48 am 
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Thank you very much for your comment 75RR and billbillt,


75RR, I have not a chance to measure it, the Oblique illumination look 3 dimensions to me especially the small water drop near diatom.


billbillt, Yes, I like the effect and it look like DIC in some degree, for 40X Objective.



Suphot


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PostPosted: Thu Jun 14, 2018 7:44 am 
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billbillt wrote:
Great image!... Oblique attempts to mimic DIC, and this one approaches that.. It does not have the perfectly crisp outline that makes DIC shine, but great pic... Thanks for sharing...


Shouldn't that be DIC mimics oblique? It must predate it by about a hundred years.


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PostPosted: Thu Jun 14, 2018 12:46 pm 
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photomicro wrote:
billbillt wrote:
Great image!... Oblique attempts to mimic DIC, and this one approaches that.. It does not have the perfectly crisp outline that makes DIC shine, but great pic... Thanks for sharing...


Shouldn't that be DIC mimics oblique? It must predate it by about a hundred years.



QUITE POSSIBLE.. BUT THE IMAGE QUALITY DOES NOT REACH THE LEVEL OF DIC.. IF A PERSON HAS DIC THERE IS NO REASON TO USE OBLIQUE..


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PostPosted: Thu Jun 14, 2018 1:30 pm 
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Joined: Sat Oct 25, 2014 5:32 pm
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Location: France
Bonjour
Jolie images en éclairage oblique.
Pour réaliser un beaux éclairage oblique il faut plus ou moins jouer avec le cache en carton ou plastique pour avoir plus ou moins effet rechercher.
Car suivant la nature de objet on régleras le cache en plastique pas de la même façon.
Celui que j'utilise le plus et la flèche de Mathias.
http://www.microscopies.com/DOSSIERS/Ma ... ATHIAS.htm
Elle permet avoir différent type éclairages et ça réalisation et facile à réaliser.
Cordialement seb

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