Hi all, a few images of some slides I made yesterday from tissue taken from a nice Carex.pendula AKA 'Pendulous sedge' that is growing in my garden. This is a monocotyledonous plant and has some very nice anatomy that I thought some may enjoy.
These sections were cut at 10µ and 5µ. Generally the thicker sections will show overall morphology and stain more heavily for the same staining protocol, whilst the 5µ (in this case) ones will show more fine detail and stain more lightly - a little counter-intuitive maybe, but more tissue basically means more stain!
Here are a few images, all stained with Safranin and Fast-green.
The whole cross-section from several stitched images through the 'scope...
Some interesting detail of the area around the main-vein, this image is actually from a 5µ section.
This is a region up to the edge of the leaf, with parts labelled...
I particularly like the tiny faces of the vascular bundles.... 10µ really stains brightly,
Same region at 5µ, slightly lighter stain and finer detail...
Close-up of main-vein. The torn-looking hole above the two large 'eyes' (xylem vessels) of the vascular-bundle is where a large xylem vessel has ruptured as the tissue has expanded during rapid growth - commonly seen in mature xylem.
Nice view of a vascular-bundle showing also the fiber bundles above and below the vascular-bundle, thus completing the 'strut' that includes the vascular-bundle and supports the leaf surfaces. Between these 'struts' (see 1st image and others above) mature leaves have air-spaces, slightly less mature leaves may still have parenchyma in this position.
Forgot this image,
That's all I have for now, I only sectioned the leaves and also some of a large 'Day Lily' (Hemerocallis.fluva) in my garden. At this time I'm building a modest collection of slides showing different types of leaf, these and the Lily being monocotyledonous, together with the anatomical/morphological features that adapt them to their particular natural environments.
I should have a few images from the Lily sections soon also.
Hope you like them!