Stauroneis phoenicenteron (COL-DIC)

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75RR
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Stauroneis phoenicenteron (COL-DIC)

#1 Post by 75RR » Wed Dec 04, 2019 7:05 am

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Planapo 63x/1.4, COL and DIC, 100µm length stacked in Photoshop

This is from a Klaus Kemp 8 Form slide

Not the fairest of comparisons as I used the position III phase annulus in the condenser to achieve COL and it is a little small for best results with the 63x
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Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

tgss
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Re: Stauroneis phoenicenteron (COL-DIC)

#2 Post by tgss » Wed Dec 04, 2019 3:09 pm

Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.

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75RR
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Re: Stauroneis phoenicenteron (COL-DIC)

#3 Post by 75RR » Wed Dec 04, 2019 8:12 pm

Thanks tgss

Even though the COL would be better with a larger annulus I think it still shows it to be a good step up on brightfield.
Zeiss Standard WL (somewhat fashion challenged) & Wild M8
Olympus E-P2 (Micro Four Thirds Camera)

Hobbyst46
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Re: Stauroneis phoenicenteron (COL-DIC)

#4 Post by Hobbyst46 » Wed Dec 04, 2019 10:39 pm

Both images are very nice !
On the one hand, the DIC appears to be 3D, on the other hand, I remember that it is not necessarily true.
The COL on the other hand is quite similar to phase contrast. I guess that it is nearer the "true" image (where true=SEM, but only my guess).
Zeiss Standard GFL+Canon EOS-M10, Olympus VMZ stereo

tgss
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Re: Stauroneis phoenicenteron (COL-DIC)

#5 Post by tgss » Thu Dec 05, 2019 12:23 pm

Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.

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