Ranunculus (Buttercup) pollen

Ranunculus repens pollen in bright field
Ranunculus repens pollen in dark field
Ranunculus repens pollen in dark field
Ranunculus repens pollen in bright field
Ranunculus repens pollen in bright field
Ranunculus repens

The top image was taken using dark field, the bottom one using bright field. This plant is poisonous and can cause skin irritation. The name “Crowfoot” comes from the shape of the leaves, which resemble the claws of a crow.

Now a few words concerning sample preparation. The pollen was collected by dusting the flower over a microscopic glass slide. The pollen was briefly dried in open air (about 1 hour) and then permanently mounted in Euparal mounting medium. The standard mounting medium for pollen is Glycerin gelatin, which is water based. I assume that the drying and the Euparal caused the pollen to shrink somewhat, but I yet have to make a comparison with fresh pollen.

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