Microscopy Podcast

This instructional video gives an introduction into the correct use of the microscope.

Here I show you how to make different filters for your microscope.

Darkfield microscopy is one of the simplest and cheapest contrast enhancing techniques. It works well for specimens that have a refractive index which is different from its surrounding medium, but which are difficult to see because they lack color. Dark field microscopy shows the specimen bright on a dark background.

The condenser aperture diaphragm (or iris diaphragm) is used to control the contrast and resolution of an image. This article explains the usage of the diaphragm.

Koehler illumination ensures that the specimen receives a bright uniform light. Only those areas actually seen are illuminated.

In this post, the function of the condenser aperture diaphragm is explained. The purpose of the condenser is to concentrate the light onto the specimen, its diaphragm regulates resolution, contrast and depth of field.