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	<title>MicrobeHunter.com &#187; iodine</title>
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	<link>http://www.microbehunter.com</link>
	<description>of Microscopy, Microbes and More</description>
	<lastBuildDate>Wed, 08 Sep 2010 10:00:43 +0000</lastBuildDate>
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		<title>Stains and reagents for microscopy</title>
		<link>http://www.microbehunter.com/2010/01/12/stains-and-reagents-for-microscopy/</link>
		<comments>http://www.microbehunter.com/2010/01/12/stains-and-reagents-for-microscopy/#comments</comments>
		<pubDate>Tue, 12 Jan 2010 13:30:32 +0000</pubDate>
		<dc:creator>Oliver Kim</dc:creator>
				<category><![CDATA[Labwork]]></category>
		<category><![CDATA[Recommended reading]]></category>
		<category><![CDATA[eosine]]></category>
		<category><![CDATA[haematoxylin]]></category>
		<category><![CDATA[iodine]]></category>
		<category><![CDATA[methylene blue]]></category>
		<category><![CDATA[reagents]]></category>
		<category><![CDATA[staining]]></category>
		<category><![CDATA[stains]]></category>

		<guid isPermaLink="false">http://www.microbehunter.com/?p=1461</guid>
		<description><![CDATA[I found an article in Popular Science Magazine (see link below) which gives a general overview of different stains that can be used in microscopy. The article divides the stains into three categories: Common household chemicals: this includes Iodine, for example. They are very readily available. Substances used mostly for microscopy: Methylene blue, Hematoxyline, and [...]]]></description>
			<content:encoded><![CDATA[<p>I found an article in Popular Science Magazine (see link below) which gives a general overview of different stains that can be used in microscopy. The article divides the stains into three categories:</p>
<ul>
<li><strong>Common household chemicals:</strong> this includes Iodine, for example. They are very readily available.</li>
<li><strong>Substances used mostly for microscopy:</strong> Methylene blue, Hematoxyline, and Eosine belong to this group.</li>
<li><strong>Commercial substances:</strong> they are sold by companies specializing in microscopic chemicals.</li>
</ul>
<p>The article also provides a step-by-step guide on how to stain a blood sample (don&#8217;t do this in schools due to danger of infection).</p>
<p>Link to the article: <a href="http://books.google.com/books?id=gCgDAAAAMBAJ&#038;pg=PA70&#038;lr=&#038;as_drrb_is=q&#038;as_minm_is=0&#038;as_miny_is=&#038;as_maxm_is=0&#038;as_maxy_is=&#038;num=30&#038;as_brr=1&#038;rview=1&#038;cd=1#v=onepage&#038;f=false" onclick="pageTracker._trackPageview('/outgoing/books.google.com/books?id=gCgDAAAAMBAJ_038_pg=PA70_038_lr=_038_as_drrb_is=q_038_as_minm_is=0_038_as_miny_is=_038_as_maxm_is=0_038_as_maxy_is=_038_num=30_038_as_brr=1_038_rview=1_038_cd=1_v=onepage_038_f=false&amp;referer=');">Help Your Microscope with Stains and Reagents (Popular Science, March 1937)</a></p>
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		<item>
		<title>Observing Potato Starch Grains</title>
		<link>http://www.microbehunter.com/2009/01/18/observing-potato-starch-grains/</link>
		<comments>http://www.microbehunter.com/2009/01/18/observing-potato-starch-grains/#comments</comments>
		<pubDate>Sun, 18 Jan 2009 19:20:30 +0000</pubDate>
		<dc:creator>Oliver Kim</dc:creator>
				<category><![CDATA[Labwork]]></category>
		<category><![CDATA[iodine]]></category>
		<category><![CDATA[potato]]></category>
		<category><![CDATA[starch]]></category>

		<guid isPermaLink="false">http://microscopy.okim.info/?p=966</guid>
		<description><![CDATA[Potato starch grains are an ideal for observation in polarized light and in dark-field. Sample preparation is simple and straight-forward.]]></description>
			<content:encoded><![CDATA[<p><div class='summary'>Potato starch grains are an ideal for observation in polarized light and in dark-field. Sample preparation is simple and straight-forward.</div><br />
<strong>Materials:</strong> a potato, kitchen knife, slides, cover slips, water, iodine.</p>
<p><strong>Method:</strong></p>
<ol>
<li>Cut the potato in half and scrape a little of the potato onto the microscope glass slide. This can be done either with a knife or with the fingernails. There should not be any large potato pieces on the glass.</li>
<li>Place a small drop of water on the &#8220;potato juice&#8221; and then place the glass cover slip on top.</li>
<li>Observe using the microscope. The starch grains will be visible as oval structures.</li>
<li>Now dilute a small amount of iodine in some water. The water should only turn slightly yellow. Place a drop of the dilute iodine next to the glass cover glass, so that some of the solution is able to flow between the cover glass and the slide.</li>
<li>You should be able to see how the starch grains change color. The iodine will react with the starch and turn it blue-black.</li>
<li>Alternatively, you can observe the starch grains in dark field or in polarized light (without adding iodine): <a href="http://microscopy.okim.info/2008/12/darkfield-microscopy/ " onclick="pageTracker._trackPageview('/outgoing/microscopy.okim.info/2008/12/darkfield-microscopy/?referer=');">Darkfield Microscopy</a> | <a href="http://microscopy.okim.info/2008/12/simple-polarization-microscopy/" onclick="pageTracker._trackPageview('/outgoing/microscopy.okim.info/2008/12/simple-polarization-microscopy/?referer=');">Simple Polarization Microscopy</a> | <a href="http://microscopy.okim.info/2009/01/potato-stach-grains/" onclick="pageTracker._trackPageview('/outgoing/microscopy.okim.info/2009/01/potato-stach-grains/?referer=');">Potato Starch Grains</a></li>
</ol>
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