Posts that relate to the Keyword: pollen

Dandelion pollen in dark-field

The pollen grains from a dandelion (Taraxacum sp.) were collected and air-mounted (no liquid mounting medium used). Eleven separate images were stacked together to increase the depth of field and to produce the final image. The color contrast was then adjusted. Dark-field patch stop was used.

Safe sources of microorganisms for microscopy

A simple check of the refrigerator (or the super market) provides many safe sources for microorganisms to view under the microscope.

The effect of the mounting medium on specimen and image quality

The mounting medium can have a significant effect both on the image quality and on the specimen itself. I tried a little experiment by observing pollen from a plant (in this case the buttercup, Ranunculus), mounted in five different ways: Air-mounted, with no cover glass Air-mounted, with a cover glass Mounted in water (temporary mount) [...]

Ranunculus (Buttercup) pollen

Spring time is pollen time! Here are two images of Ranunculus repens (the Creeping Buttercup or Creeping Crowfoot) pollen, the top one in dark field, the bottom one in bright field. This plant is poisonous and can cause skin irritation. The name “Crowfoot” comes from the shape of the leaves, which resemble the claws of [...]

Making mounts of pollen grains

Permanent slides of pollen grains can be used as a reference for identifying unknown pollen samples. It is therefore important, that the pollen grains remain in an authentic, natural shape. The preparation and mounting of the pollen can introduce artifacts: the pollen may lose some of its pigment, start to shrink and shrivel or absorb [...]