The refractive indes (RI) of the mounting medium can have a significant impact on the image quality.
Here is an instruction on how to calculate the size of cells using a microscope.
There are several methods how bubbles can be reduced when making slides.
Cutting a carrot with a hand microtome.
Here I’m unpacking my new hand microtome and explaining the different parts. A hand microtome (or cylinder microtome) is a device used to make thin cuts of a specimen for microscopic observations. The video briefly illustrates the different parts of a hand microtome.
This post explains how to make a wet mount. Video included!
Before specimens can be processed for making permanent slides, they may need to be fixed.
A wet mount (or temporary mount) is one of the most common ways of observing specimens under the microscope.
Permanent slides of pollen grains can be used as a reference for identifying unknown pollen samples. It is therefore important, that the pollen grains remain in an authentic, natural shape.
Here I will give an overview of the different factors that may be used to decide on which mounting medium to choose.