Search found 744 matches

by Wes
Mon Apr 19, 2021 3:49 pm
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 128

Re: Actinocyclus octonarius

. Thanks MichaelG. and Hobbyst46 Is the 12.5X KPL raised above parfocality height ? Is it a 12.5x -W high eyepoint KPL ? Raised 10mm by the collar shown. High eyepoint (as indicated by the eyeglass pictogram) but believe field of view is 18mm . Great shot! Why do you need the collar exactly? What h...
by Wes
Tue Apr 06, 2021 1:57 pm
Forum: For forum members who want to buy and sell equipment
Topic: FS: Zeiss Pol 25/0,45
Replies: 0
Views: 312

FS: Zeiss Pol 25/0,45

I offer a classical tension-free Zeiss 25/0,45 objective for polarization work. The collar runs smoothly and optics are in excellent condition (no delamination, fungus, scratches etc.). Some minor cosmetic blemishes can be seen but they do not affect the performance. Cost is 95 eur + shipping. Best ...
by Wes
Tue Apr 06, 2021 1:51 pm
Forum: Microscopes and optics
Topic: Zeiss Photomicroscope DIC Inko questions.
Replies: 7
Views: 358

Re: Zeiss Photomicroscope DIC Inko questions.

I'd be interested to see some examples of the level of DIC that you achieve with your system.
by Wes
Mon Apr 05, 2021 7:56 pm
Forum: Specimens, samples and slides
Topic: Using aluminium tape to make spacers for water sample specimens
Replies: 11
Views: 556

Re: Using aluminium tape to make spacers for water sample specimens

I use a syringe with petroleum jelly/vaseline to make a fine border on the coverslip. Make sure to leave a gap so when you press on the coverslip with a needle ot fine tweezers the extra water has a passageway to escape your chamber. Thus you can regulate the thickness of the sample and apply just e...
by Wes
Mon Apr 05, 2021 5:11 pm
Forum: Microscopes and optics
Topic: Zeiss Photomicroscope DIC Inko questions.
Replies: 7
Views: 358

Re: Zeiss Photomicroscope DIC Inko questions.

I think this kind of DIC depends on specific objectives (e.g. Plan 40/0.65) and it only works within particular serial number brackets of those particular objectives.

Definitely try to regular nosepiece turret if you have it handy.
by Wes
Sun Apr 04, 2021 1:36 pm
Forum: Specimens, samples and slides
Topic: How to explain it?
Replies: 16
Views: 761

Re: How to explain it?

Rossf wrote:
Sun Apr 04, 2021 10:34 am
Wes-Either way they are interesting looking invaders! Is this done in DIC? Nice images and video.
Regards
Thanks. Yes, this is taken in DIC.
by Wes
Sun Apr 04, 2021 10:29 am
Forum: Specimens, samples and slides
Topic: How to explain it?
Replies: 16
Views: 761

Re: How to explain it?

Hey Wes I wonder if the amoeba is infected or they are just food doing for a ride until big enough food vacuole is formed-or did the amoeba perish from these invaders Regards Ross Yes I considered it but the host amoeba doesn't look healthy at all, its just sitting there, rounded up not moving at a...
by Wes
Sat Apr 03, 2021 4:30 pm
Forum: Microscopes and optics
Topic: Remedy for eyepiece delamination
Replies: 6
Views: 332

Re: Remedy for eyepiece delamination

I have no idea what the Zeiss standard of accuracy was. Well the Germans are known for their engineering standards and attention to details so I imagine fairly tight tolerances. I can't remember where I saw it but modern high end objectives being assembled by hand using tiny hammers to nudge the le...
by Wes
Fri Apr 02, 2021 9:13 pm
Forum: Microscopes and optics
Topic: Remedy for eyepiece delamination
Replies: 6
Views: 332

Re: Remedy for eyepiece delamination

The ultimate way to fix delaminated eyepieces would be to soak the doublets in hexane and once they separate apply fresh lens cement. . Assuming that you have the equipment/skill to centre the two elements to the original standard. MichaelG. How would you go about that? Its a topic I'm quite curiou...
by Wes
Fri Apr 02, 2021 9:03 pm
Forum: Microscopes and optics
Topic: Remedy for eyepiece delamination
Replies: 6
Views: 332

Re: Remedy for eyepiece delamination

I tried that, it works...sort of. I can definitely see residual defects in the optics that become more obvious with time but still significantly better than before applying the oil. Don't know if sealing the edges with say canada balsam would improve the ever so slightly gradual deterioration of the...
by Wes
Fri Apr 02, 2021 7:53 pm
Forum: Pictures and Videos
Topic: Protozoa Cell division?
Replies: 5
Views: 325

Re: Protozoa Cell division?

This doesn't look like a regular division. Very interesting.
by Wes
Fri Apr 02, 2021 3:04 pm
Forum: Pictures and Videos
Topic: Protozoa Cell division?
Replies: 5
Views: 325

Re: Protozoa Cell division?

Video is set to private.
by Wes
Mon Mar 29, 2021 6:33 pm
Forum: Beginner's corner
Topic: A trinocular phase contrast Zeiss standard on eBay
Replies: 8
Views: 384

Re: A trinocular phase contrast Zeiss standard on eBay

It does look 'crusty' but, you are getting a Standard 18 with 5 objective turret, a trinocular head, phase condenser and lighting with power source. Something a person could work with. Sure if the parts you mention are in a good condition its a bargain. But if they are all riddled with fungus, rust...
by Wes
Sun Mar 28, 2021 8:49 pm
Forum: Specimens, samples and slides
Topic: Where to buy slides and #1.5 coverslips
Replies: 12
Views: 2202

Re: Where to buy slides and #1.5 coverslips

If you are looking at aquatic samples, # 1 coverslips are often more suitable. The recommendation for using #2 covers is based on an ideal but many amateurs prepare aquatic samples too thick. For higher resolution objectives .40 N.A. and over but especially .65 and over using thinner coverslips , ....
by Wes
Sun Mar 28, 2021 8:31 pm
Forum: Beginner's corner
Topic: A trinocular phase contrast Zeiss standard on eBay
Replies: 8
Views: 384

Re: A trinocular phase contrast Zeiss standard on eBay

One thing that I have spotted is a bit of corrosion on some of the screws. The objectives are crusty as well. As things on ebay tend to go bad rather than good and given the shabby appearance of the scope I'd make a guess and say it doesn't look like a particularly good deal Perhaps an unpopular op...
by Wes
Fri Mar 26, 2021 9:30 pm
Forum: Miscellaneous
Topic: E-Bay question when selling
Replies: 3
Views: 235

Re: E-Bay question when selling

Not sure about your ebay predicament but got curious about the object of desire in this dodgy transaction :lol:
by Wes
Fri Mar 26, 2021 9:22 pm
Forum: Specimens, samples and slides
Topic: How to explain it?
Replies: 16
Views: 761

Re: How to explain it?

I like this thread! For many reasons, this type of periodontal clinical microscopy died out in the 1980's. I'm sure there are still many weird organisms living in there, unknown to science, but no one is looking for them any more. But why would people willingly abandon such a research niche? Seems i...
by Wes
Fri Mar 26, 2021 2:27 pm
Forum: Specimens, samples and slides
Topic: So how do you prep a tuna slide?
Replies: 3
Views: 143

Re: So how do you prep a tuna slide?

Thats a cool channel that one can easily slip into binge watching. They use a well slide in this section but I suppose if you want to do this properly i.e. quantitatively I'd suggest you hydrolyse the biological material with hydrochloric acid, then centrifuge to collect the plastic bits and stain w...
by Wes
Fri Mar 26, 2021 12:50 pm
Forum: Camera systems and imaging
Topic: How to attach a modern camera to this Zeiss Universal accessory?
Replies: 7
Views: 372

Re: How to attach a modern camera to this Zeiss Universal accessory?

What you need is a Zeiss West 43.5 mm dovetail to either a male M42 or T2 adapter (see picture below). What worked for me was to add a M42 female to 52 mm male thread adapter but that could be any thread your camera lens accepts. As Bob had advised me at some point I bought a Canon 40 mm pancake len...
by Wes
Wed Mar 24, 2021 9:44 pm
Forum: Illumination Techniques
Topic: Water/Glycerine immersion instead of oil immersion for darkfield
Replies: 33
Views: 1012

Re: Water immersion instead of oil immersion for darkfield

Interesting test, thanks. I am working on plotting glass-water-glass transmission vs. NA (somewhat separate issue from aberrations) as mentioned in the other thread but not finished yet. Might be useful to try to separate effect of watering the condenser vs. objective in this sort of experiment? I ...
by Wes
Wed Mar 24, 2021 9:39 pm
Forum: Beginner's corner
Topic: Anyone else use Air Purifiers?
Replies: 1
Views: 140

Re: Anyone else use Air Purifiers?

Yes and I can't recommend one more. Absolutely fantastic, totally worth the 200 or 300 bucks I paid for it (came with a fancy HEPA filter and whatnot). I've been thinking about getting a de-humidifier as well, seems like a logical extension of my efforts in environmental control (the purifier actual...
by Wes
Wed Mar 24, 2021 8:09 pm
Forum: Microscopes and optics
Topic: Interesting article: Getting the best out of a basic microscope
Replies: 7
Views: 356

Re: Interesting article: Getting the best out of a basic microscope

This is some pretty high end imaging for a microscope without condenser. Thanks for posting.
by Wes
Wed Mar 24, 2021 7:52 pm
Forum: Illumination Techniques
Topic: Water/Glycerine immersion instead of oil immersion for darkfield
Replies: 33
Views: 1012

Re: Water immersion instead of oil immersion for darkfield

Now that is a well controlled comparison. Thanks for posting Hobbyst. Number 11) looks like a top of the line homogenous immersion system, really nice and crispy. I only have the universal condenser D position darkfield option at my disposal and I've used it with water which produced ok results at l...
by Wes
Sun Mar 21, 2021 9:21 am
Forum: Pictures and Videos
Topic: Closterium ehrenbergii
Replies: 6
Views: 252

Re: Closterium ehrenbergii

Great video with really nice detail from the DIC. Thanks for sharing.
by Wes
Fri Mar 19, 2021 5:55 pm
Forum: Miscellaneous
Topic: Diatoms and homicide.
Replies: 7
Views: 547

Re: Diatoms and homicide.

Very morbid, interesting nontheless. I find it cool that you can link a specific crime scene locale to the species identified during a forensic pathology investigation.
by Wes
Fri Mar 19, 2021 8:05 am
Forum: Beginner's corner
Topic: Condensers, how do you properly use them?
Replies: 17
Views: 774

Re: Condensers, how do you properly use them?

I use water instead of oil on the condenser for high NA objectives, its a decent compromise (according to my math it allows a cone of light with NA=1.2) ... Is that number less than 1.33 because it is based on some criteria like 50% transmission rather than critical angle? I never said I was good i...
by Wes
Thu Mar 18, 2021 5:50 pm
Forum: Beginner's corner
Topic: Condensers, how do you properly use them?
Replies: 17
Views: 774

Re: Condensers, how do you properly use them?

- I read Abbe condensers have to be oiled with higher than NA 1.0 objectives; is that so? can all abbe condensers be oiled? - Do you need an apo condenser to use high end apo lenses? like those 60/1.40 Oil ones.. I use water instead of oil on the condenser for high NA objectives, its a decent compr...