Search found 630 matches

by microb
Wed Aug 04, 2021 9:01 am
Forum: Microscopes and optics
Topic: Phase contrast insert for objective?
Replies: 4
Views: 138

Re: Phase contrast insert for objective?

Came across this listing for a Leitz EF 10x objective with a "phase contrast insert": https://www.ebay.com/itm/384310270761?epid=1725023138&hash=item597aad5b29:g:0CEAAOSw995hCXFu I don't see how that insert could possibly work for phase contrast. Does anyone know what it really is? For it to be a L...
by microb
Tue Aug 03, 2021 2:18 pm
Forum: Illumination Techniques
Topic: Optics and Polarization Light Source Question
Replies: 2
Views: 91

Optics and Polarization Light Source Question

Will polarized light run through and properly cross-polarize given a path of lenses like those in an epi that might introduce barrel distortion, and who knows, maybe Olympus distresses the glass when they make their lamp to epi lenses. A BX61 RLAA has a heavily curved lens aimed towards the lamp at ...
by microb
Mon Aug 02, 2021 4:10 pm
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 127

Re: RMS and SM1 Threads on Fusion 360

The angle was wrong in the file, so I created a separate file for RMS with 55 degrees. <?xml version="1.0" encoding="UTF-8"?> <ThreadType> <Name>Imperial Microscopy threads</Name> <CustomName>RMS Imperial Microscopy Threads</CustomName> <Unit>in</Unit> <Angle>55</Angle> <SortOrder>5</SortOrder> <Ext...
by microb
Mon Aug 02, 2021 10:21 am
Forum: Camera systems and imaging
Topic: Video Editing Software
Replies: 17
Views: 6933

Re: Video Editing Software

Ben Krasnow for his Applied Science channel says he uses a free license of Resolve: https://youtu.be/ihbYtxaEDSk?t=752
by microb
Sun Aug 01, 2021 10:55 pm
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 127

Re: RMS and SM1 Threads on Fusion 360

Olympus U-P4RE brass adjustable centering mount for an RMS objective, with adjusting pins and rubber plug when a fixed disk is used with no pins.
by microb
Sun Aug 01, 2021 1:09 am
Forum: Home-made microscope adaptations
Topic: RMS and SM1 Threads on Fusion 360
Replies: 2
Views: 127

RMS and SM1 Threads on Fusion 360

If you need to do threads in Fusion, this posting gives an xml file that is a must and so far appears to be working. https://forums.autodesk.com/t5/fusion-360-design-validate/how-can-i-make-an-internal-rms-royal-microscopy-society-0-8-quot/m-p/8888486/highlight/false https://knowledge.autodesk.com/s...
by microb
Sat Jul 24, 2021 7:21 pm
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 311

Re: Wired vs Geared Stages

For the Olympus BX 'scopes the wired stages I think have no overhang when moved to the limit in the X-axis. This 'overhang' of the rack has caught me out a few times, and I'd really like a wire-operated stage which doesn't have this problem - but it's finding one for my BX50 and of course affording...
by microb
Sat Jul 24, 2021 7:13 pm
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 311

Re: Wired vs Geared Stages

MichaelG. wrote:
Sat Jul 24, 2021 5:06 am
This is a tentative response, Ted … because there is nothing currently pictured in your post

A wire system can be built [at reasonable cost, and without excessive friction] to be completely free of backlash.

MichaelG.
Fixed it. I changed the image format so the website likes it.
by microb
Sat Jul 24, 2021 12:12 am
Forum: Microscopy accessories
Topic: Wired vs Geared Stages
Replies: 6
Views: 311

Wired vs Geared Stages

What is the advantage of a wired stage like the one pictured here. Apparently some people abhor the older style rack pinion design and only want a stage with a wire mechanism. If the gearing is done right, I don't set that the wire and pullies would be that much different in feel. Thanks, Ted [So th...
by microb
Fri Jul 23, 2021 10:45 am
Forum: Pictures and Videos
Topic: Diatoms in epi DIC
Replies: 18
Views: 462

Re: Diatoms in epi DIC

What does a Leica z6 with Rottermann look like?
by microb
Fri Jul 23, 2021 2:57 am
Forum: Illumination Techniques
Topic: DIC Question
Replies: 211
Views: 8449

Re: DIC Question

To control the strain applied and not damage the surface, wouldn’t it be better to cut the poly sheet to have supports with more surface area that a rig can make contact with. For example, instead of press point from a rod, what about spike with these blue surfaces to interface with some metal that ...
by microb
Wed Jul 21, 2021 5:56 am
Forum: Illumination Techniques
Topic: DIC Question
Replies: 211
Views: 8449

Re: DIC Question

Link is missing... I know I had it there, so I edited it above. What I thought was interesting is that he molded the lens and where the plastic was pushed in there was more stress. So he built into the plastic a level of stress. I wonder if that could be done but I guess the rig with the screws is ...
by microb
Tue Jul 20, 2021 7:42 pm
Forum: Illumination Techniques
Topic: DIC Question
Replies: 211
Views: 8449

Re: DIC Question

Breaking Taps had a video showing the fringing on a molded acrylic lens, which I think was actually polypropylene:

https://youtu.be/JNXgHbBIRYQ?t=1016

[I know I had added the link, but it disappeared. So added it.]
by microb
Sun Jul 18, 2021 4:07 pm
Forum: Miscellaneous
Topic: How really safe is a laser pointer ? new findings
Replies: 6
Views: 236

Re: How really safe is a laser pointer ? new findings

Cheap green laser will not have an IR filter. Inside they convert from 808nm to 532nm, and a spectrometer will show both peeks. The IR frequency is especially not eye safe. This video shows how to test without a spectrometer: https://www.youtube.com/watch?v=iR1Ku5dnbH8 So if you are cooking your sam...
by microb
Sat Jul 17, 2021 9:46 pm
Forum: For forum members who want to buy and sell equipment
Topic: AX70 frame forsale for parts
Replies: 1
Views: 124

AX70 frame forsale for parts

Hi, I have an AX70 frame that I tested objective and filter cube turrets on, plus motored transmission filter. I haven't test sparking lamps just yet. But anyway, the main thing is that the vertical focus knobs are too loose on the right and disconnected on the left. The right side knob does move th...
by microb
Sat Jul 17, 2021 12:14 am
Forum: Camera systems and imaging
Topic: Olympus BX/AX light path to camera diameter
Replies: 1
Views: 69

Re: Olympus BX/AX light path to camera diameter

<r>So I don't have a DIN retarder plate, but if they are 20mm wide, then the filter has to something down in the 17mm range.<br/> <br/> "Modern first order retardation plates are built in a frame having standardized DIN dimensions (6 × 20 millimeters) that will enable their use in a variety of micro...
by microb
Fri Jul 16, 2021 10:01 pm
Forum: Camera systems and imaging
Topic: Olympus BX/AX light path to camera diameter
Replies: 1
Views: 69

Olympus BX/AX light path to camera diameter

For the filters like the analyzer and the filter cubes in a BX setup, a 25mm filter diameter is used. Now the AN does a 5 degree slant for the polarizer glass to minimize some reflection, so it's light path diameter is actually elliptical, cut down to just under 24mm. So clearly Olympus didn't reall...
by microb
Thu Jul 15, 2021 6:42 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

This book has a lot of interesting stuff in it. The sample is also assumed to rrtard light by about a quarter wave. I haven't yet managed to understand the arguments in Bennett's book for why this is true and the "Molecular Expressions" primer and related manufacturer-specific sites don't really ex...
by microb
Thu Jul 15, 2021 6:41 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

patta wrote:
Thu Jul 15, 2021 8:36 am
Also posts #6 (book link, pag. 87) and #11 plz
Cool. For some reason I did not see those posts till just now. The forum UI was hiding it from me.

Thanks,
Ted
by microb
Wed Jul 14, 2021 4:42 am
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 43
Views: 1245

Re: Questions about metallurgy on Olympus

I wouldn't retrofit this setup. The halogen bulbs are cheap and easy to source and give off good light. My second choice is retrofitting a fiber optic light guide from a 150w source. Retrodiode is good but I'm not sure about the color temperature, or if I'd want to use a pwm-dimmed LED for photogra...
by microb
Tue Jul 13, 2021 12:40 am
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

For gravitational waves, phase plates can be made out of galaxies. Well I'm still searching for little bits of galaxy to sputter or cut from a dielectric that give me a visible spectrum Zernike phase plate. I was hoping some of the papers would have citations back to something but I didn't find any...
by microb
Mon Jul 12, 2021 2:31 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

This article uses Zernike zone plates, but gives materials, but this probably just for x-rays: "The substrate for each of the zone plates was a 100 nm thick silicon nitride membrane with a plating base consisting of 5 nm chromium and 12 nm gold. H" https://www.osapublishing.org/DirectPDFAccess/07663...
by microb
Mon Jul 12, 2021 2:23 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

So I think I found something to read through tonight: https://arxiv.org/ftp/arxiv/papers/1708/1708.05151.pdf "Previous Zernike phase-plates, for transmission-electron-microscopy (TEM) have always been made out of carbon. A critical constraint on those phase-plates is their fast degradation during us...
by microb
Mon Jul 12, 2021 1:41 pm
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

If one puts two orthogonal quarter waveplates together So putting two quarter waveplates together matching fast to slow axis by adding a 90 degree rotation between them is called a compound waveplate. Zero-order compound quarter waveplates are made to control for frequency and temperature. Nothing ...
by microb
Mon Jul 12, 2021 5:39 am
Forum: Illumination Techniques
Topic: 50W LEDs
Replies: 10
Views: 369

Re: 50W LEDs

MichaelG. wrote:
Sun Jul 11, 2021 8:11 pm
Thanks for that Saul … but I have a plan :geek:

MichaelG.
Beam reducer, run it through a metre of fiber to mix, and then shoot the results into a lens to create a collimated light source.

Or do something else I guess....
by microb
Sun Jul 11, 2021 5:04 pm
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 43
Views: 1245

Re: Questions about metallurgy on Olympus

I see. Where can I buy these prism mount screws? Do you have a link to where I may get some? They normally come with the prism. This e-bay item for example. I don't know the seller. I just wanted a reference image: https://www.ebay.com/itm/Olympus-Microscope-Nomarski-DIC-Prism-50X-ULWD-/173262708023
by microb
Sat Jul 10, 2021 4:42 pm
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 43
Views: 1245

Re: Questions about metallurgy on Olympus

I still don't see how I'm to power this. On ebay I see lots and lots of BH2-UMA's for sale, but none of them come with power supply nor can I find the bh2 tsg transformer that's needed to power it. Surely people have a solution to this? Here are the typical lamps and power supplies for the UMA. I d...
by microb
Thu Jul 08, 2021 8:02 pm
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 43
Views: 1245

Re: Questions about metallurgy on Olympus

Here some links that give part numbers and function for BH2 metallurgical/reflectance: http://www.alanwood.net/downloads/olympus-bh2-uma-instructions.pdf http://www.alanwood.net/downloads/olympus-bhm-bh-2-brochure.pdf http://www.alanwood.net/downloads/olympus-bh2-parts-diagrams-intermediate-tubes.pd...
by microb
Thu Jul 08, 2021 4:05 am
Forum: Illumination Techniques
Topic: Positive Phase Contrast Objective Phase Plates
Replies: 47
Views: 1061

Re: Positive Phase Contrast Objective Phase Plates

2,516,905 - Microscope with variable polarizing and birefringent means for producing contrast in optical images 2,700,918 - Microscope with variable means for increasing the visibility of optical images These are cool. So instead of using masks, they used rings of different polarizations and a comp...