Search found 192 matches

by dtsh
Fri Mar 05, 2021 1:59 am
Forum: My microscope
Topic: My very first compound microscope!
Replies: 22
Views: 326

Re: My very first compound microscope!

Your best bet is to use the 40X with 15X eyepieces. The correct ones , cat. # 182 will give you an excellent well corrected image with the 40X and lower objectives. Am I looking for AO or Reichert branded eyepieces, or both? Technically the 182 eyepieces, which are the 15x eyepiece engineered for i...
by dtsh
Thu Mar 04, 2021 7:21 pm
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

Re: AO 20x comparison

I opened the images in tabs to compare while flipping back and forth but they are the same ones as in the first post -- files got mixed up? Fail again, yes they are the first ones. One more time, that's a second datapoint for anyone else counting. :P In my defense I was in a hurry to get out hte do...
by dtsh
Thu Mar 04, 2021 3:39 am
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

Re: AO 20x comparison

That would seem as it should , I would think, relating cover to no cover objectives. The fog starts to form at about .40 and by .50 it's pretty evident. .... There seems to be a bit of pincushion distortion with them all and it is skewed a little...... Camera lens? Camera being a cellphone on an ad...
by dtsh
Thu Mar 04, 2021 3:35 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB X-drive from Zeiss rotating stage 4227544
Replies: 7
Views: 104

Re: WTB X-drive from Zeiss rotating stage 4227544

If you can't find a replacement, it might be worth reaching out to the folks at hobby-machinst.com and other machining forums as I am pretty sure that can be salvaged. Probably someone no too far from you who might be able to assist in repair or fabrication.
by dtsh
Wed Mar 03, 2021 11:53 pm
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

Re: AO 20x comparison

Now that I've embarassed myself, let me see if I can at least pull it out of the fire and provide some slightly more accurate information. For starters, working distance is as reported; facts being what they are, there's one more datapoint in support of me being a fool. The same objectives, this tim...
by dtsh
Wed Mar 03, 2021 3:22 pm
Forum: Beginner's corner
Topic: Compound Microscope for Gross Anatomy Observation?
Replies: 32
Views: 491

Re: Compound Microscope for Gross Anatomy Observation?

That's good to know. I wasn't sure how easily it would be to pick up additional objectives given that they are infinity plans and I don't really understand how they all relate to each other. I do not see "neo" on the objectives currently on the scope? Or do I need an Neoplan to work with this scope...
by dtsh
Wed Mar 03, 2021 2:01 pm
Forum: Digital processing
Topic: rotating cylinder to surface
Replies: 11
Views: 153

Re: rotating cylinder to surface

MichaelG. wrote:
Tue Mar 02, 2021 3:39 pm
I suspect that the combination of a line-scan camera and a stepper motor would be a very good place to start.
I don't know about the OP, but thank you for leading me to that rabbithole.
by dtsh
Wed Mar 03, 2021 1:43 pm
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

Re: AO 20x comparison

The 1077 and 2556 were nearly equal, I could rotate the turret between the two and the focus was nearly identical... This is establishing that the parfocal distance (nominally 34 mm) is the same. Could try measuring working distance directly using the scale on the fine focus knob while carefully lo...
by dtsh
Wed Mar 03, 2021 4:21 am
Forum: My microscope
Topic: My very first compound microscope!
Replies: 22
Views: 326

Re: My very first compound microscope!

Glad to see you have it. The best test of the system is to use it. Check to make sure all the moving parts move smoothly without grinding, catching, or slipping and that it produces a clean crisp image when in focus. I presume you have a slide and a coverslip? If so, do the usual and find something,...
by dtsh
Wed Mar 03, 2021 4:11 am
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

Re: AO 20x comparison

Might be worth trying with cover glass to see how much difference it makes for the 1022 and 1077. There is very little difference at 10X 0.25 but the difference is obvious at 40X 0.66. Not sure about 20X 0.5. Do you have any immersion oil? I usually stick a piece of cover glass to the calibration s...
by dtsh
Tue Mar 02, 2021 11:12 pm
Forum: Digital processing
Topic: rotating cylinder to surface
Replies: 11
Views: 153

Re: rotating cylinder to surface

Look into image stitching would be my guess. What about "no depth of field" with microscope? When i observe cylinder, i have sharp screen only in focused area (belt). So i have to stich tens of slices... If you're lucky only 10's; that's the cost of fidelity in this regard. The higher the magnifica...
by dtsh
Tue Mar 02, 2021 8:18 pm
Forum: Beginner's corner
Topic: Compound Microscope for Gross Anatomy Observation?
Replies: 32
Views: 491

Re: Compound Microscope for Gross Anatomy Observation?

Just received the microscope! I've looked it over and to the best of my limited knowledge, it seems to be in working condition! The light works, the blue filter works, the ND works, and the diaphragm works! I didn't realize it, but all of the objectives are infinity plan achros. I have two addition...
by dtsh
Tue Mar 02, 2021 3:02 pm
Forum: Digital processing
Topic: rotating cylinder to surface
Replies: 11
Views: 153

Re: rotating cylinder to surface

Look into image stitching would be my guess. There's plenty of software, both free and commercial, to do various forms of this sort of image manipulation. There may be better methods suggested by someone who's actually done it, but that's where I would start. Edit: If you're really dedicated, perhap...
by dtsh
Tue Mar 02, 2021 1:46 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB AO 10 illuminator mirror
Replies: 6
Views: 131

Re: WTB AO 10 illuminator mirror (resolved)

Hobbyst46 wrote:
Tue Mar 02, 2021 7:29 am
Relevant previous post:
viewtopic.php?f=5&t=5995&p=53980&hilit= ... tic#p53980
Thanks much for the added info. Marking this as resolved.

This place is great, if every community were like this the world would be a much nicer place.
by dtsh
Tue Mar 02, 2021 3:37 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB AO 10 illuminator mirror
Replies: 6
Views: 131

Re: WTB AO 10 illuminator mirror

apochronaut wrote:
Tue Mar 02, 2021 2:58 am
You can buy orthodontic photo mirrors of polished stainless steel on ebay for very little. Very high reflectivity. Not too hard to cut and if you buy the right one there is lots of material to play with.
That would have never crossed my mind, thanks. I'll look into it.
by dtsh
Tue Mar 02, 2021 12:56 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB AO 10 illuminator mirror
Replies: 6
Views: 131

Re: WTB AO 10 illuminator mirror

I doubt you could find a replacement. When I had that problem - mine had lost its silvering, which all first surface mirrors do eventually - I searched for and purchased the thinnest first surface mirror I could find on eBay. It was thicker and far too large, but thickness doesn't matter as long as...
by dtsh
Tue Mar 02, 2021 12:51 am
Forum: Microscopes and optics
Topic: AO 20x comparison
Replies: 11
Views: 254

AO 20x comparison

I happen to have in my possession 3 different 20x objectives (34mm infinity) for the American Optical Series 10/20/100/110/120 Series microscopes. The three objectives are catalog numbers 1022, 1077, and 2556. In previous attempts to look and compare I had noticed that the 1077 and the 2556 seemed t...
by dtsh
Mon Mar 01, 2021 5:28 pm
Forum: For forum members who want to buy and sell equipment
Topic: FOUND - WTB - Compound microscope for viewing pond water microbes
Replies: 6
Views: 212

Re: WTB - Compound microscope for viewing pond water microbes

I have microscopes with and without the trinocular head. If imaging is a huge interest then I think a tinocular head would be a necessity, but an inexpensive cellphone adapter works fine for imaging. No, they aren't as fancy, but to truly take advantage of the trinocular head typically requires sign...
by dtsh
Mon Mar 01, 2021 5:22 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB AO 10 illuminator mirror
Replies: 6
Views: 131

WTB AO 10 illuminator mirror

An instrument I recently purchased arrived with the illuminator mirror having become detached and had shattered. Anyone have a decent one of these to spare or know of a good source for an appropriate fisrt surface mirror to replace it with?
by dtsh
Mon Mar 01, 2021 5:17 pm
Forum: Beginner's corner
Topic: Compound Microscope for Gross Anatomy Observation?
Replies: 32
Views: 491

Re: Compound Microscope for Gross Anatomy Observation?

Assuming all is in working order I think you will be very pleased. I also have a Microstar IV and have been nothing but pleased with it, the optics are very nice. Parts availability is pretty good for the common parts, but some of th more unusual configurations seem to be hard to find. I know trinoc...
by dtsh
Mon Mar 01, 2021 6:39 am
Forum: Beginner's corner
Topic: Compound Microscope for Gross Anatomy Observation?
Replies: 32
Views: 491

Re: Compound Microscope for Gross Anatomy Observation?

Thanks everyone! Does anyone have any thoughts on this listing? I'd prefer to buy from someone here, but there's nothing going on at the moment. https://www.ebay.com/itm/Motic-BA300-Phase-contrast-Binocular-Compound-Microscope-with-4-Objectives-/392714315029 I don't know Motic well, but that looks ...
by dtsh
Fri Feb 26, 2021 11:15 pm
Forum: Home-made microscope adaptations
Topic: AO Cycloptic Illuminator
Replies: 3
Views: 118

Re: AO Cycloptic Illuminator

I was pretty pleased with the results.

Here's a shot of one of my tabanids at 25x as an example.
25x_tabanid.jpg
25x_tabanid.jpg (123.81 KiB) Viewed 76 times
by dtsh
Fri Feb 26, 2021 10:05 pm
Forum: Introduce yourself
Topic: Hello! Help me with my new AO Microscope?
Replies: 4
Views: 101

Re: Hello! Help me with my new AO Microscope?

A lot of times phase contrast stuff is expensive in bits and pieces and cheaper as a whole package, so keep an eye out for another series 4 with phase.
I feel sad every time I strip a stand for parts, but sometimes it's the most effective way to get what you want.
by dtsh
Fri Feb 26, 2021 8:31 pm
Forum: Pictures and Videos
Topic: Can you see RBC'S with a stereo?... Barely
Replies: 5
Views: 120

Re: Can you see RBC'S with a stereo?... Barely

What scope and relative magnifications is that?
by dtsh
Fri Feb 26, 2021 2:54 pm
Forum: Pictures and Videos
Topic: Can you see RBC'S with a stereo?... Barely
Replies: 5
Views: 120

Re: Can you see RBC'S with a stereo?... Barely

I can make out RBCs in my Cycloptic even at relatively low magnifications, though they appear as little more than specs until around 40x; at 80x I can start to discern a little detail. I can't comment on anything higher as 80x is the limit for the Cycloptic with a 2x barlow
by dtsh
Fri Feb 26, 2021 3:45 am
Forum: Home-made microscope adaptations
Topic: AO Cycloptic Illuminator
Replies: 3
Views: 118

AO Cycloptic Illuminator

I have an illuminator I use with my Cycloptic,one of the gooseneck types. It works fine, but the goosenecks are forever in my way. When I recently acquired a second Cycloptic, I decided to do something different, partly because this one is destined to spend it's time elsewhere and I don't want to bu...
by dtsh
Thu Feb 25, 2021 1:55 pm
Forum: Microscopes and optics
Topic: AO Industrial Series
Replies: 6
Views: 130

Re: AO Industrial Series

There is this attributed to Gregg Kleinberg on the "AO History" page of P.S. Neeley's site: The AO 3000, which was a modular "industrial body" with reflected light, was based on the Series 10 architecture. The only main difference was that the higher power objectives were not cover slip corrected. ...
by dtsh
Thu Feb 25, 2021 1:18 am
Forum: Microscopes and optics
Topic: AO Industrial Series
Replies: 6
Views: 130

AO Industrial Series

I've became aware of the "Industrial Series" in my quest for a 20x for my brightfield scope, an AO 10 Series which I started looking at on the recommendation of members. I'm used to the universe providing me with "just missed it" opportunities, so I was surprised to find myself in posession of a 102...
by dtsh
Wed Feb 24, 2021 7:55 pm
Forum: Specimens, samples and slides
Topic: LockMailer jars
Replies: 0
Views: 72

LockMailer jars

I stumbled across these a little while back while looking at Coplin jars (https://www.simport.com/en/products/133-m950.html) so I decided to try them out. Some of the aspects I liked about them were the size, much of my staining is one or two slides at a time and it often leads to a fair bit of wast...
by dtsh
Wed Feb 24, 2021 7:15 pm
Forum: Pictures and Videos
Topic: Hi sweetie, I've been looking for you!
Replies: 5
Views: 135

Re: Hi sweetie, I've been looking for you!

My old nemesis in a new perspective. Nice image and glad it ended up where it belongs, dead on a slide for our amusement.