Search found 265 matches

by dtsh
Thu Apr 15, 2021 3:54 pm
Forum: Microscopy accessories
Topic: A quick review of a 3V-12V 5A powersupply
Replies: 10
Views: 600

Re: A quick review of a 3V-12V 5A powersupply

DrPhoxinus, Thanks for the info! Have been purchasing the 50 year old transformers for the AO microscopes and it is hit or miss if they work. One of power supplies you listed will serve about 7 different power ranges on my microscopes! Have also been trying to switch out the high wattage lamps to t...
by dtsh
Thu Apr 15, 2021 3:44 pm
Forum: Microscopes and optics
Topic: Afocal adaptor
Replies: 4
Views: 210

Re: Afocal adaptor

I designed and 3D printed a piece that clamps onto the eyepiece and the camera. The intent was to machine one when I'm certain it is how I want it, but it's been working fine thus far so little motivation to change it. I went this route as I wasn't sure at first what sort of spacing would be require...
by dtsh
Tue Apr 13, 2021 11:42 pm
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 5
Views: 304

Re: Newbie learns to take images, dtsh edition

A slightly better result from stacking.
Dermacentor_hypostome.jpg
Dermacentor_hypostome.jpg (95.44 KiB) Viewed 65 times
by dtsh
Tue Apr 13, 2021 7:39 pm
Forum: Beginner's corner
Topic: Could someone help with a slide specimen?
Replies: 2
Views: 95

Re: Could someone help with a slide specimen?

Looks like epithelial cells to me, bacteria are typically much, much smaller than animal or plant cells and unless using specialized tools, usually require staining to help differentiate them from the background. To say something is "normal" necessitates knowing details of what it is, how it was pre...
by dtsh
Tue Apr 13, 2021 3:08 pm
Forum: Beginner's corner
Topic: Very dirty Nikon microscope, what to do?
Replies: 10
Views: 298

Re: Very dirty Nikon microscope, what to do?

I enjoy taking things apart, but I'm worried about ruining something in THIS thing. I know that taking a tablesaw apart, I can use Sim ple Green and whatever, and the only problem is remembering to install all the parts where they go, and to dry, oil, and lubricate it. A microscope (especially of t...
by dtsh
Tue Apr 13, 2021 1:05 pm
Forum: Beginner's corner
Topic: Very dirty Nikon microscope, what to do?
Replies: 10
Views: 298

Re: Very dirty Nikon microscope, what to do?

If I work on it, I'll spend another $50 on cleaning stuff like lens paper and 70% ethanol, and specially designed swabs and whatnot, and gain the experience and better understanding, and I'd I ruin it, I still have all the accessories like the condenser, stage, etc, for a LOT less money. A jug of d...
by dtsh
Tue Apr 13, 2021 12:51 pm
Forum: Introduce yourself
Topic: Newbie
Replies: 15
Views: 722

Re: Newbie

Some photos of the microscope. Is there a better way to post photos than the attachments thing? I had to go into a photoshop program and shrink these to the SMALLEST jpg size I could find. Some use imgur.com, photobucket, or whatever, I just resize them for here as I hate it when remote images vani...
by dtsh
Tue Apr 13, 2021 3:51 am
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 5
Views: 304

Re: Newbie learns to take images, dtsh edition

Today's image is another single image, this time it's the first ick of the season. For this one I was focusing on he hypostome (the central part with the hooks) A quick look makes me think it might be an Amblyomma species, but there are a few things that don't seem quite right. I'll look into it mor...
by dtsh
Mon Apr 12, 2021 7:58 pm
Forum: My microscope
Topic: Received my stereo microscope today.
Replies: 6
Views: 178

Re: Received my stereo microscope today.

Nice to hear. I tend to use the stereo a lot more than the compound. So many small items I tend to inspect and it's so much nicer than a hand loop. It's also nice to be able to explore things that aren't flat or forced to be flat. It's a wholly different view from the compound; they do the same thin...
by dtsh
Sun Apr 11, 2021 3:29 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 526

Re: can Phase contrast kit work WITHOUT Köhler ???

Photos taken with 10x eyepieces (afocal with cellphone)
by dtsh
Sun Apr 11, 2021 2:35 am
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 5
Views: 304

Re: Newbie learns to take images, dtsh edition

Today's attempt is a single image of pollen from a Stellaria spp using the 40x objective, mounted in G-PVA.
Stellaria_pollen2.jpg
Stellaria_pollen2.jpg (119.87 KiB) Viewed 194 times
by dtsh
Sat Apr 10, 2021 7:29 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 526

Re: can Phase contrast kit work WITHOUT Köhler ???

Hello , I have a big question for the one who own a phase contrast kit : Could try to see if it still work when your microscope isnt set in Köhler illumination setting ? I cant understand why I could get my x40 Ph LOMO works perfetly with no problm and not do the same with the LOMO Ph x10 ??? (of c...
by dtsh
Sat Apr 10, 2021 4:52 pm
Forum: Introduce yourself
Topic: Newbie
Replies: 15
Views: 722

Re: Newbie

I just got it in the mail! WOW, it weighs like 30 pounds! I have only used the terrible old microscopes that had the mirror, one focus knob, and not even the sun could produce enough EM radiation to resolve an image in it lol. This one is VERY dirty. It has been badly neglected, but I'm looking aro...
by dtsh
Fri Apr 09, 2021 9:01 pm
Forum: Beginner's corner
Topic: Amscope T340B-DK vs. Amscope T490B-DK
Replies: 19
Views: 431

Re: Amscope T340B-DK vs. Amscope T490B-DK

InstantMochiii, I was thinking the same thing, just wondering if the amount of light will be ok with 3W led vs. 30W halogen. 3W should be sufficient for regular brightfield, but may be insufficient when using filters that block a lot of light or when using light intense methods such as darkfield. A...
by dtsh
Fri Apr 09, 2021 8:51 pm
Forum: Pictures and Videos
Topic: Newbie learns to take images, dtsh edition
Replies: 5
Views: 304

Newbie learns to take images, dtsh edition

I am very much the newie when it comes to imaging, so please don't expect too much. My intent is to have a place to put what I've learned and to have a spot where I can look back on what I have accomplished (or not!). I have a few different microscopes, but I will try to mention exactly which one is...
by dtsh
Fri Apr 09, 2021 4:34 pm
Forum: Beginner's corner
Topic: Amscope T340B-DK vs. Amscope T490B-DK
Replies: 19
Views: 431

Re: Amscope T340B-DK vs. Amscope T490B-DK

InstantMochiii, I was thinking the same thing, just wondering if the amount of light will be ok with 3W led vs. 30W halogen. 3W should be sufficient for regular brightfield, but may be insufficient when using filters that block a lot of light or when using light intense methods such as darkfield.
by dtsh
Fri Apr 09, 2021 12:47 pm
Forum: Beginner's corner
Topic: Amscope T340B-DK vs. Amscope T490B-DK
Replies: 19
Views: 431

Re: Amscope T340B-DK vs. Amscope T490B-DK

Thank you all for your input. I think I have decided! The Amscope T490A. As Red Green said, it would save money and patch stops are faster to change. I like that it has 15X eyepieces and I plan to buy a 20X and 60X objective. Perhaps drop the 4X from the turret. It is HARD to choose a microscope! I...
by dtsh
Wed Apr 07, 2021 9:59 pm
Forum: My microscope
Topic: Taking pictures with a stereo scope
Replies: 2
Views: 365

Re: Taking pictures with a stereo scope

I just bought a stereo microscope to be used as a dissection microscope mostly. How do you take pictures with a stereo microscope? Any special techniques, just in case. In my very limited experience, exactly as with a trinocular compound; my experience is so slim one could probably fairly assess it...
by dtsh
Wed Apr 07, 2021 9:53 pm
Forum: Introduce yourself
Topic: Newbie
Replies: 15
Views: 722

Re: Newbie

How long have you been into photography? I've been at it since 2008, but only got into DSLRs in 2012, and that was just normal photography, not micrographs and stuff A few months at best and even that half-heartedly. I have mostly used a cellphone at the eyepiece, which has worked great for me, but...
by dtsh
Wed Apr 07, 2021 8:25 pm
Forum: Beginner's corner
Topic: what is a 1x objective ?
Replies: 5
Views: 469

Re: what is a 1x objective ?

I believe hans is correct. For example on my Cycloptic, the CMO (Common Main Objective) is 1x, next up the path is the magni-changer where I can select 7x, 10x, 15x, 20x, or 25x, then on up to the prisms and eyepieces. If I select 10x as the magnification, the magnichanger is just air with no elemen...
by dtsh
Wed Apr 07, 2021 7:24 pm
Forum: Introduce yourself
Topic: Newbie
Replies: 15
Views: 722

Re: Newbie

Hello and welcome!

From what I have seen the Nikon is likely to be a nice instrument. I'm just begining to explore photography myself, so I look forward to learning along with you.
by dtsh
Wed Apr 07, 2021 7:02 pm
Forum: My microscope
Topic: I just bought a new stereo microscope
Replies: 4
Views: 444

Re: I just bought a new stereo microscope

DonSchaeffer wrote:
Wed Apr 07, 2021 6:17 pm
I am going to use it for wet slide preparation mostly. I'm using a simple eyeglass magnifier now--not enough magnification and working distance.
I don't know that model, but I think you will enjoy it. I look forward to seeing images from it, too.
by dtsh
Wed Apr 07, 2021 6:49 pm
Forum: Beginner's corner
Topic: Pls help me spend my money!
Replies: 7
Views: 456

Re: Pls help me spend my money!

I would consider some 15x eyepieces over the 60x dry myself. Assuming you have the usual load of 4x, 10x, 40x, 100x it would add 6x (meh), 15x, and 60x. The rule of thumb of 1000 x NA as max usable magnification gives you more utility with 15x than it does with 20x and gives you more magnification r...
by dtsh
Wed Apr 07, 2021 12:52 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 24
Views: 1144

Re: Slide Making with 61°C Wax

I began to wonder if it was worth the effort of posting to be honest, so little interest outside of equipment and pond-water at the moment. Understandable though as the live-mounts are so accessible. I certainly enjoy them and find them very infromative. I don't frequently comment unless I think I ...
by dtsh
Tue Apr 06, 2021 4:57 am
Forum: Illumination Techniques
Topic: What am i doing wrong?
Replies: 11
Views: 450

Re: What am i doing wrong?

What brand and model of darkfield condenser are you using?
Does the manual for it (assuming there is one) have instructions for setup?
by dtsh
Mon Apr 05, 2021 10:59 pm
Forum: Illumination Techniques
Topic: What am i doing wrong?
Replies: 11
Views: 450

Re: What am i doing wrong?

What little I know of darkfield ondensers is limited to the one I have. With it I need to oil (I tend to use a drop of water) between the condenser and the bottom of the slide. It is very particular about condenser height and the condenser being centered. I have been using it with a 20w halogen, but...
by dtsh
Mon Apr 05, 2021 7:02 pm
Forum: Specimens, samples and slides
Topic: Using aluminium tape to make spacers for water sample specimens
Replies: 11
Views: 533

Re: Using aluminium tape to make spacers for water sample specimens

There are also other slides, McMaster and heamacytometer, usually used for counting, but can work for other purposes. The depth of the McMaster is probably too much for all but the largest of critters. The problem with well slides, McMaster, etc is that there's still quite a bit of depth to them and...
by dtsh
Mon Apr 05, 2021 6:32 pm
Forum: Illumination Techniques
Topic: What magnification is most popular.
Replies: 4
Views: 317

Re: What magnification is most popular.

I admit it. I'd rather have a turret mounted phase contrast system. I also admit that I can't afford it right now so suppose I get the cheaper mount where you have to change the condenser to match magnification. What would be the most used magnification?. Under those circumstances I would pretty mu...
by dtsh
Sun Apr 04, 2021 3:11 pm
Forum: Specimens, samples and slides
Topic: Counting chambers and Bogorov chambers
Replies: 2
Views: 178

Re: Counting chambers and Bogorov chambers

I have used a haemacytometer for counting and observing other cells and have used the typical 0.17 coverglass with it. It may well help with the issue. The typical 22mm x 22mm coverglasses don't cover both chambers, but they work fine with just one. There's nothing wrong with the 0.4mm ones intended...
by dtsh
Fri Apr 02, 2021 10:44 pm
Forum: Beginner's corner
Topic: Advice on buying Stereo Microscope
Replies: 44
Views: 3137

Re: Advice on buying Stereo Microscope

Hi A boom mounted head would be an ideal choice for me. Trying to identify a suitable microscope head that I can't inspect or return if faulty is a gamble, especially from e-bay. The cost of shipping will be the same regardless of the value of the microscope. Shipping cost is dead money. As time go...