MicrobeHunter.com Microscopy Forum

There is one thing that confuses me. If there was just one version of the top element for the Universal Condenser, how come I have three different tops in each of my thee Universal Condensers? A=1.3 np A=1.3 P A=0.9 P Could it be then, that the latter two tops are from another Reichert condenser, a...

For those interested, that are a number of interation of the Polyvar. the link below is a summary photo list. Some are extremely rare, like the Infrapol. A Polarized light microscope that is only presenting near IR light to the sample. No need for a bino tube. Only a IR sensitive camera. Pretty cool...

I rescanned the order guide with the missing pages 4-5. Here is a link https://drive.google.com/file/d/1HhM2HyAzNMdE8AE4c2A4quvVBtE7T9v4/view?usp=drive_link This link will take you to the Polyvar 2 4 page brochure, it is the only version I have https://drive.google.com/file/d/1hFPQUz4G04OTbKi9GY4d-n...

Attached is a copy of the Polyvar order guide from 1985. I have one from the 1990 as well, but it is for the Polyvar 2 and si twice the size. I learn by trying to post it here, it it too large for this forum to attach. I have two scans. 100DPI in BW and 200DPI gray scaled. the latter is over 6Mb in ...

The Polyvar offered three condenser for BF TL applications, The Universal condenser for DIC, Phase, BF and DF inserted. The standard condenser element for this condenser was a 5 element Aplanatic/Achromatic corrected with 1.30na or 0.90na when used dry. There was also a immersion DF condenser availa...

I jsut measured the barrel diameter of a 20x/160 Leitz objective and it is 24.5mm in diameter. Those threads appear to be slightly larger so it could be a M25 or M28 adapter. Not sure what it would have been placed on though.

Certainly appears to be a thread adapter, although I don't think the difference is M25/RMS. M25 isn't a big a difference as that appears to be. Could be an M32 or something other. It appears a spanner wrench could be used to remove the adapter, leaving you with an RMS thread, Of course the Labovert ...

Two Points, If you have a confocal system, Don't you already have fluorecent capability? Yes, the higher you stack the system there is likelyhood, you will lose FN. Both Visually and to the camera port. I don't know enough about the Olympus design, but if the they have a WF tube for the 26.5 FN, you...

It appears you have the required componnents for DIC The Polarizer, Analyser and the objective prism for the 25X L on the slider. It appears the condenser has a matching prism for the 25x (S25) as well? If so, it is complete for DIC on that one objective.

Thanks all for the input. The catalog number is 506135. I've had a hard time finding info on older Leica parts, and even the current Leica website is sparse with details on their current offerings. As a reference point. The 506135 is a LMC objective. possessing the following specs. N Plan L 40x/0.5...

do you have the catalog number for the objective. As pointed out, likely a Modulation contrast objective. also called LMC. Leica Modulcation Contrast. ala Hoffman.

All comments listed are valid, One point of caution would be not to leave the lamp in the scope or lamp house durng transport. Also do not touch the envelop of the lamp. It is not glass, and the oils from your fingers will weaken the envelop during the periods of the lamp in operation. That could le...

Interesting writeup Pete. I know you touched on some of these points in your brands PDF—which by the way was a huge help. It's still odd to me that there isn't even one reference to the DMLB model anywhere on the official Leica website. I agree with you about the move to electronic automation being...

This scope has a Dual view attachment below the viewing tube, so inserting an analyser is possible, but may require stopping down the eyepeice FN to 20mm if it is at 22 now. Depending on the viewing tube. The condenser pictured is the 11501183 Aplanatic/Achromatic 0.9na. the P indicates Strain free ...

As Pete M pointed out, the Condenser Prism and Objective Prism in this scope are for the 40x and 100x objective. I too am curious what objectives are on this scope, They don't appear to be Leica objectives. I don't recall there ever being black barrels on any Leica objectives except for the very low...

The Gold Standard for specimens that demonstrate resolving capabilities is the Pluerosigma diatom. It is very easy to see the impact of reducing the NA of the objective by closing the condenser.

These were the images I wanted to share in my earlier post. I think they can help visualize the objective NA/Condenser NA relationship. Unfortunately it does not show what happens to working distance as the NA increases. But you can see the change in tne Angular Aperture Angle increasing as the NA i...

I still have not figured out how to post images into these threads, b/c if have some that will graphically show what I discuss below. It seems there is some room to simplify the discussion. Although the subject question of Averaging the condenser and objective NA to predict resolution is being queri...

Frequently a 1x compound objective will need a contrast cap, especially for reflected light applications. A polarizer is placed in the light path, and the contrast cap is mounted over the front of the objective. It is essentially a 1/4 wave plate that can be rotated for the best contrast as you obse...

This may be QWERTY vs. Dvorak keyboards. Having gotten used to field numbers, FN certainly eems simple enough. Use a 10x/22 eyepiece and I expect to see a 22mm wide field with a 1x stereo objective and a 2.2mm field with a 10x compound objective. Put a ruler or micrometer slide down and take a look...

This may be QWERTY vs. Dvorak keyboards. Having gotten used to field numbers, FN certainly eems simple enough. Use a 10x/22 eyepiece and I expect to see a 22mm wide field with a 1x stereo objective and a 2.2mm field with a 10x compound objective. Put a ruler or micrometer slide down and take a look...

In my forty years of working in microscopy, I have never heard of or had defined what an “image circle” is relative to the image size. And frankly the description in this thread makes it even less certain. Apochromat, I can’t continue with this thread, you are conflating and confusing recognized mic...

F.N. is an arbitrary system. It is a code system stamped on some eyepieces, only some. There seems to be a begrudging compliance on the part of manufactuers. Maybe there is a reason? In existence, and you can look them up on ebay right now and find at least some of these, so they sre pretty common ...

I am now beginning to see how ts has been confusing. Scarodacyl’s is a fair description of what happens in a microscope system. If one were to take the eyepiece out of the system, with an infinity objective, with a bright enough light source the image would be projected to the ceiling. The magnifica...

I have two of these objectives. It is difficult to image wihout glare, but i can tell you it says on the side. Plan 10x/0.25 bekow that printing is the infinity sign,/- np on one and a P on the other. Yours has the IK, all three labels were used to indicate strain free status. On the opposite side, ...

Phil, i need to do some of my own testing. i am not sure what you mean by Base 10. Our math system when using metric is base 10. I don't know the scale of the micrometer you are picturing, but it all of the images you took are with the 10x objective. The objective will see the same distance, that is...

I am trying to do the math and it doesn't work for me. IF you used a 1x objective with a 16mm FN , 15x eyepiece and put a stage micrometer in place, you would observe a 16mm FOV at 15x. If you put used the same objecive with a 10x/20mm FN, you would see a 20mm FOV at 10x. The object would appear to ...

I have a 580 with photo adapter, and 1x mount 10x eyepieces. Also OEM style focusing post stand I also have a ring light or double branch guide fiber optic illuminator? I may have some other illuminator pieces, but need to sort that out, like vertical illuminator mirror housing. Works with 369 illum...

When I reference the FN, I am referring to the field stop diameter of the eyepiece. If you had a 1x objective, with a FN eyepiece of 20mm, you would be able to observe a stage micrometer field of 20mm, if the objective was a 2x, the sample are viewed would be 10mm. It is just as important that the r...

All very good information, Wayne. You certainly fill in a lot of gaps. After you help out Pete, with his D.I.C. quest, could you comment on this. One thing I don't see factored in, is the objective distance to the telan lens. The reference focal length,probably the same referred to in patents as th...