MicrobeHunter.com Microscopy Forum

Don.
I hope to be back soon reading your nice poems.
Get well and strengthen your being fully.

Hi Sabatini, what you have there are Leitz (160 or 170mm)and Zeiss Jena 160mm microcopes. For the Leitz microscope you need Leitz Periplan eyepieces as they contain a certain degree of colour correction. With Zeiss Jena it is a bit more difficult. Your objectives will be on one of the last 160mm se...

Ok... now I understand.
Thank you very much for your help

Phill Brown wrote: ↑

Mon Apr 18, 2022 6:55 pm

They look like 30mm which might not be ok.
23.2mm is more likely what you have?

They're 22mm. In this case you think is a good choice.?

Cordial greetings. Here I come again looking help of those here always willing to expand our knowledge. These zeiss eyepieces which I show below are from Zeiss, I think primostar infinite system. The thing is that my microscopes are all 160 and 170 mm.... Both Zeiss and laitz. The question I have is...

apochronaut wrote: ↑

Tue Jan 25, 2022 2:51 am

Use a measured slide as a coverslip. There are also thick coverslips used for haemocytometers , usually .4 but I think there are thicker ones as well.

Thanks for the help.

Yes indeed... thank you.

Great images

DonSchaeffer wrote: ↑

Sat Feb 26, 2022 11:07 pm

neat images.

Thanks You Don. I Like very much your poems.

By recommendation of Hobbyst46...sr, I couldn't see them with epifluorescence...so I will still be able to show them in Bright field.
.

The I2/3 is for blue light excitation in a band of 450 - 490 nm. Very good for specific fluorescence stains but somewhat limited on chlorophyl auto-fluorescence. A blue LED in the 460 - 470 nm range would be best for this filter cube. Buy some acridine orange. It is the perfect stain for this filte...

The 2.5/0.08 is a central part of your problem. This objective is totally useless for fluorescence. Use your Fluotars. They are much better suited to the job. What filter cubes do you have and which one did you use? btw: Nice microscope! This thing will give you a lot of pleasure and reward. Thank ...

Incidentally : Do the posted photos show that the light from the 100W LED comes from below the light port below the condenser ? if so, the setup is not epi-fluorescence but trans-illumination. If so, the chance of seeing any FL in the shown setup is zero, whatever the objective in use, whatever the...

Kind regards to all. As we all know in this beautiful world of microscopes, our mind is always searching for better optical quality and this keeps us in the search and expectation of finding and obtaining better optics. Now in this search, I managed to buy two nice microscopes a laborlux S and a lab...

Nice

Great images!

I appreciate your sincerity and willingness to keep the integrity of the objective intact.
I will adhere to your recommendation.
Thank you.

Thank you gentlemen for your excellent contributions and different solid points of view. I will not use any solvent for the moment I will try to remove the leftovers with a scalpel and possibly put them in some solvent to see what the results are. The detective work to find out by prints the possibl...

The front element is sealed into it's mount or bezel, often by furling an interior section of it over the extreme edge of the front element's rear hemisphere. The amount of metal is very thin and undue pressure on the front lens can push it through the furled ring. Cementing the lens with epoxy is ...

BramHuntingNematodes wrote: ↑

Mon Jan 31, 2022 5:41 pm

this also happens when the careless microtomist crashes a lens into a newly made slide with uncured mountant

It can certainly be a good possibility
Thank you!

Cordial greetings . I just purchased this leitz 25 x EF, it's giving a clear and contrasted images, I was cleaning it and noticed that it has some leftover glue like possibly Canada balsam by the color on the front element. But I wonder if it is possibly it has been recementing the front element or ...

Excellent solution, very interesting...Of course I will also be putting it into practice.
Thank you!

Use a measured slide as a coverslip. There are also thick coverslips used for haemocytometers , usually .4 but I think there are thicker ones as well. Ah okay ,now I understand ,I have to make a sandwich with two slides , one below and one above, measured from 0.6 onwards. Great solution and very s...

Cordial greetings.
Does anyone have a solution for using this objective on a non-inverted microscope?

Hello.
Try this...https://en.m.wikipedia.org/wiki/Cerium(IV)_oxide

Fantastic!!
Thank you and welcome

Great work

Cordial greetings to all and a new year full of good projects. Although I have read about the possible solution to this problem from the experience of several here on the forum always ready to collaborate. well I would still like to try a solution , which among other things I know probably lead me t...

Great...!!!