Search found 1547 matches
- Sat Apr 27, 2024 12:11 am
- Forum: Illumination Techniques
- Topic: A modern take on Koehler
- Replies: 4
- Views: 602
Re: A modern take on Koehler
Oh yeah I been using those thorlabs aspheric lenses with my LEDs for awhile now they're great and not too pricey.
- Sat Apr 13, 2024 4:02 pm
- Forum: Specimens, samples and slides
- Topic: Dry Mounted Insect
- Replies: 8
- Views: 3084
Re: Dry Mounted Insect
Dry mounts are good for low power observation. The optical reason you might want a resin or wet mount might be to maintain more homogenous optical medium when using immersion oil. Also, especially for in vertebrate skeletons, chemical processing can allow for flattening, and scopes love a flat subje...
- Sat Apr 13, 2024 3:52 pm
- Forum: Camera systems and imaging
- Topic: What accessory do I need to par focal microscope camera in trinocular tube of stereo microscope ?
- Replies: 13
- Views: 1047
Re: What accessory do I need to par focal microscope camera in trinocular tube of stereo microscope ?
The reduction sizes are also important to match the image projected and the size of your sensor. If you have a very small sensor and use the 1x the image will be blurry. If you have a full size sensor and use too much reduction you will get a small circle of image but the rest will be black. You can...
- Wed Apr 10, 2024 10:26 pm
- Forum: Microscopes and optics
- Topic: Brand of this old condenser?
- Replies: 16
- Views: 2174
Re: Brand of this old condenser?
Well this lister thinks this is a dark field condenser and says it also might be a Leica? So maybe they don't have.it labeled oll korrect.
- Tue Apr 09, 2024 3:01 am
- Forum: Beginner's corner
- Topic: Looking for help on buying a microscope.
- Replies: 15
- Views: 4241
Re: Looking for help on buying a microscope.
Well nematodes are pretty transparent so phase can help you find them or look at stuff like their stylet bulb a little better but morphological if of nematodes is p hard but phase is good for lots of stuff in soil like bacteria shapes even but you are able to peer into transparent things and see the...
- Sat Apr 06, 2024 3:43 pm
- Forum: Microscopes and optics
- Topic: Lomo lamp & green filter
- Replies: 3
- Views: 1146
Re: Lomo lamp & green filter
There is also a reasonably flat portion of the focal length/ frequency function of the humble achromat lens around the green wavelength. Predominantly green light can make black and white photos of subjects through aachromat lenses very sharp.
- Tue Apr 02, 2024 4:31 am
- Forum: Microscopes and optics
- Topic: Refurbishing B&L Stereozoom 7
- Replies: 14
- Views: 2986
Re: Refurbishing B&L Stereozoom 7
Might have a copy of that one around here somewhere. I can look this weekend.
- Mon Apr 01, 2024 1:04 am
- Forum: Microscopy accessories
- Topic: B&L Stereozoom SZ7 accessiories
- Replies: 7
- Views: 1463
Re: B&L Stereozoom SZ7 accessiories
I have tried to see if the .5 lens from the regular line would work on the sz7 and it was unsuccessful. It may be that the different attachment points were introduced to prevent mixing the lenses.
The 2x sz7 is decently common, and you could probably find one for around $50 with a bit of patience
The 2x sz7 is decently common, and you could probably find one for around $50 with a bit of patience
- Sun Mar 31, 2024 4:16 am
- Forum: Microscopy accessories
- Topic: B&L B Stand
- Replies: 8
- Views: 941
Re: B&L B Stand
You could try white paper. It's not extremely reflective and really designed to have the Nicholas shining right one it.
- Tue Mar 26, 2024 12:18 am
- Forum: Microscopes and optics
- Topic: Substituting LED bulb for incandescent bulb
- Replies: 7
- Views: 2490
Re: Substituting LED bulb for incandescent bulb
It's never too bright
- Mon Mar 25, 2024 6:43 pm
- Forum: Microscopes and optics
- Topic: Substituting LED bulb for incandescent bulb
- Replies: 7
- Views: 2490
Re: Substituting LED bulb for incandescent bulb
The wiring'll be fine, you already noted that it will pull half the wattage. There are sometimes issues when filament position is critical, but I don't think it's too sensitive with the optilume.
- Mon Mar 18, 2024 5:49 am
- Forum: Microscopy accessories
- Topic: Anti-Static for lenses.
- Replies: 26
- Views: 3253
Re: Anti-Static for lenses.
I seem to recall one of the diatomists here using that anti static gun to help when positioning things.
- Tue Dec 05, 2023 12:06 am
- Forum: Beginner's corner
- Topic: Good Stereo Microscope?
- Replies: 36
- Views: 23466
Re: Good Stereo Microscope?
I like the b&l sz7 but you might want to stay away if you aren't a hands in kind of person as almost all of them need a little work. An sz4 is cheaper and they made a million of them. Sometimes can be had for very little. They're not the most superb imaging available but they are madly convenient. T...
- Thu Oct 12, 2023 2:36 pm
- Forum: Microscopes and optics
- Topic: Different Nikon 20/0.75 160 Plan Apos
- Replies: 14
- Views: 4094
Re: Different Nikon 20/0.75 160 Plan Apos
I don't think Nikon ever made a cheap plan apo.
- Wed Oct 11, 2023 12:25 am
- Forum: Beginner's corner
- Topic: Equipment for the microscopy hobby
- Replies: 11
- Views: 3911
Re: Equipment for the microscopy hobby
Good forceps
- Thu May 25, 2023 4:58 am
- Forum: Microscopes and optics
- Topic: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
- Replies: 16
- Views: 3218
Re: Buying decision: Amscope T670Q, Nikon Eclipse Si, Motic BA310E
Yeah you oughta talk to Apochronaut about buying direct if you're contemplating laying down six or seven thou on a microscope. A little more legwork maybe but might be worth your time.
- Thu May 25, 2023 2:50 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2884
Re: Types of Phase Contrast and when to use which type
Yeah the black knobbly base. Some are Bausch and Lomb, some are Leica.
- Thu May 25, 2023 2:18 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2884
Re: Types of Phase Contrast and when to use which type
Hmm well they're marked 160. In any case, they're pretty good but not worth going out of your way to accommodate.
- Thu May 25, 2023 1:42 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2884
Re: Types of Phase Contrast and when to use which type
Ah right, I meant the kyowa ones. I have seen that I miller has some of the Olympus lenses but I have never handled one, nor have I even seen a photo of the Galen Olympus turret.
- Thu May 25, 2023 12:13 am
- Forum: Illumination Techniques
- Topic: Types of Phase Contrast and when to use which type
- Replies: 12
- Views: 2884
Re: Types of Phase Contrast and when to use which type
The Galen phase lenses are not compatible with either the regular B&L phase or the flat field. The regular and flat field phase lenses/ diaphragms are close, except the 10x. The Galen lenses are pretty good looking plan dark medium phase achromat lenses! It is uncommon to see the proper turret, part...
- Wed May 24, 2023 7:40 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 9410
Re: How many microscopes are enough? :<)
The 1942 B&L
- Wed May 24, 2023 4:47 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 9410
Re: How many microscopes are enough? :<)
The difference in hoarding and collecting is organization and storage
- Thu May 11, 2023 9:46 pm
- Forum: Microscopes and optics
- Topic: Inverted vs Stereoscope or Compound for Pond water organisms
- Replies: 51
- Views: 31237
Re: Inverted vs Stereoscope or Compound for Pond water organisms
AN inverted scope is best used to look at things that sink to the bottom so the can press up against the glass, or things you need to access while looking at them. You can get LWD condensers with NA of .5 readily-- they are pretty big and heavy and don't always fit. I use a .4 Nikon condenser when u...
- Sat May 06, 2023 2:38 pm
- Forum: Microscopy accessories
- Topic: Amateur microscopy and professional micrometer
- Replies: 13
- Views: 3439
Re: Amateur microscopy and professional micrometer
Yeah, using a big competent microtome the 10 μm setting is for course shaping of the block, then you set it to about half of that to cut the ribbon. Thinner than 5 μm is what you might call "ultramicrotomy," but in any case even your regular microtome should do about an order of magnitude thinner (a...
- Fri May 05, 2023 12:48 pm
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 8029
Re: Epi-Fluorescence Microscopy and Deconvolution
I guess I am confused, does Photoshop have a deconvolution algorithm? I assumed that was what was being discussed as gnu image manipulation program has a genuine one and it is merely a free clone of whatever version Photoshop was at a few years ago.
- Fri May 05, 2023 12:43 pm
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 8029
Re: Epi-Fluorescence Microscopy and Deconvolution
I am not immediately familiar with Photoshop, but I assume when Berdan said he was using Photoshop deconvolution he was using some implementation of a deconvolution algorithm in Photoshop, not one of photoshops non-deconvolution algorithms. I would argue that a method that estimates a psf just from ...
- Thu May 04, 2023 1:57 pm
- Forum: Microscopes and optics
- Topic: Zeiss GFL compatible objectives?
- Replies: 17
- Views: 2648
Re: Zeiss GFL compatible objectives?
The epiplan hd objectives require a special set up, larger threads and different lighting. No problem, the others work well. If I'm not mistaken, all Zeiss objectives from that era were designed to work with compensating eyepieces, so they should be interchangeable. The neofluars are famous for.bein...
- Wed May 03, 2023 12:16 am
- Forum: Pictures and Videos
- Topic: Epi-Fluorescence Microscopy and Deconvolution
- Replies: 19
- Views: 8029
Re: Epi-Fluorescence Microscopy and Deconvolution
Call me a numbskull, but I don't understand how you'd deconvolve an image without having a solid grip on how the image was convoluted in the first place. I guess that why you'd (have to) figure out the point spread function of your optical system and then feed that into a specifically designed de-c...
- Wed Apr 12, 2023 11:45 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 9410
Re: How many microscopes are enough? :<)
Gonna want a stain scope for doing your stains it gets messy and chemically so good to have a separate scope
- Fri Mar 31, 2023 5:07 am
- Forum: Microscopes and optics
- Topic: Poor man’s "Kohler" illumination
- Replies: 41
- Views: 6544
Re: Poor man’s Kohler illumination
Also, if you get a chance to read the paper I think you will find that a "poor man's Kohler" is exactly Kohler, as he had limited equipment but a good understanding of the principles and a knack for ingenious improvisation.